EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.60 | expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.60 | ligand-free, N-acetylmannosamine-bound and substrate analog GlcNAc-bound enzyme, sitting drop vapor diffusion method, using 0.2 M sodium malonate dibasic monohydrate and 20% (w/v) PEG 3350 | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.60 | Y160A | the mutant has a higher enzyme specificity (kcat/Km) with GlcNAc than for N-acetylmannosamine | Staphylococcus aureus |
2.7.1.60 | Y160F | the mutant has a higher enzyme specificity (kcat/Km) with GlcNAc than for N-acetylmannosamine | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.60 | 0.049 | - |
N-acetyl-D-glucosamine | mutant enzyme Y160F, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 0.057 | - |
N-acetyl-D-glucosamine | mutant enzyme Y160A, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 0.26 | - |
N-acetyl-D-glucosamine | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 0.3 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 0.73 | - |
ATP | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 1.27 | - |
N-acetyl-D-mannosamine | mutant enzyme Y160F, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 1.34 | - |
N-acetyl-D-mannosamine | mutant enzyme Y160A, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 15 | - |
D-glucose | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.60 | 62000 | - |
sedimentation velocity analysis | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.60 | ATP + N-acetyl-D-mannosamine | Staphylococcus aureus | - |
ADP + N-acetyl-D-mannosamine 6-phosphate | - |
r | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | Staphylococcus aureus PS 47 | - |
ADP + N-acetyl-D-mannosamine 6-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.60 | Staphylococcus aureus | Q2G159 | - |
- |
2.7.1.60 | Staphylococcus aureus PS 47 | Q2G159 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.60 | Q-Sepharose column chromatography, phenyl-Sepharose column chromatography and Superdex S200 gel filtration | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.60 | ATP + D-glucose | - |
Staphylococcus aureus | ADP + D-glucose 6-phosphate | - |
r | |
2.7.1.60 | ATP + D-glucose | - |
Staphylococcus aureus PS 47 | ADP + D-glucose 6-phosphate | - |
r | |
2.7.1.60 | ATP + N-acetyl-D-glucosamine | - |
Staphylococcus aureus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
r | |
2.7.1.60 | ATP + N-acetyl-D-glucosamine | - |
Staphylococcus aureus PS 47 | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
r | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Staphylococcus aureus | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
r | |
2.7.1.60 | ATP + N-acetyl-D-mannosamine | - |
Staphylococcus aureus PS 47 | ADP + N-acetyl-D-mannosamine 6-phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.60 | homodimer | small-angle X-ray scattering | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.60 | N-acetylmannosamine kinase | - |
Staphylococcus aureus |
2.7.1.60 | N-acetylmannosamine-6-phosphate 2-epimerase | - |
Staphylococcus aureus |
2.7.1.60 | nanK | - |
Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.60 | 17.8 | - |
N-acetyl-D-glucosamine | mutant enzyme Y160A, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 19.1 | - |
N-acetyl-D-glucosamine | mutant enzyme Y160F, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 23.4 | - |
N-acetyl-D-glucosamine | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 60 | - |
D-glucose | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 216 | - |
N-acetyl-D-mannosamine | mutant enzyme Y160F, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 223 | - |
N-acetyl-D-mannosamine | mutant enzyme Y160A, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 262.6 | - |
ATP | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 262.6 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.60 | 4 | - |
D-glucose | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 90 | - |
N-acetyl-D-glucosamine | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 167 | - |
N-acetyl-D-mannosamine | mutant enzyme Y160A, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 171 | - |
N-acetyl-D-mannosamine | mutant enzyme Y160F, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 310 | - |
N-acetyl-D-glucosamine | mutant enzyme Y160A, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 390 | - |
N-acetyl-D-glucosamine | mutant enzyme Y160F, at pH 8.5 and 25°C | Staphylococcus aureus | |
2.7.1.60 | 871 | - |
N-acetyl-D-mannosamine | wild type enzyme, at pH 8.5 and 25°C | Staphylococcus aureus |