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Literature summary extracted from

  • Ben Ouirane, K.; Boulard, Y.; Bressanelli, S.
    The hepatitis C virus RNA-dependent RNA polymerase directs incoming nucleotides to its active site through magnesium-dependent dynamics within its F motif (2019), J. Biol. Chem., 294, 7573-7587 .
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mg2+ the enzyme directs incoming nucleotides to its active site through Mg2+-dependent dynamics within its F motif. Mg2+-bound nucleotide first binds next to the tunnel entry of the RNA-dependent RNA polymerase(RdRp)-specific entry tunnel. Interactions with the triphosphate moiety orient the nucleotide such that its base moiety enters first. Dynamics of RdRp motifs F1 + F3 then allow the nucleotide to interrogate the RNA template base prior to nucleotide insertion into the active site. These dynamics are finely regulated by a second magnesium dication, thus coordinating the entry of a magnesium-bound nucleotide with shuttling of the second magnesium necessary for the two-metal ion catalysis Hepacivirus C

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Hepacivirus C
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Hepacivirus C
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 RNA-dependent RNA polymerase
-
 Hepacivirus C

General Information

EC Number General Information Comment Organism
2.7.7.48 drug target since virally-encoded RNA-dependent RNA polymerases of RNA viruses synthesizes new genomes in the infected host they are prime targets for antiviral therapy Hepacivirus C
2.7.7.48 physiological function virally-encoded RNA-dependent RNA polymerases of RNA viruses synthesizes new genomes in the infected host Hepacivirus C