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Literature summary extracted from

  • Steffen, W.; Ko, F.C.; Patel, J.; Lyamichev, V.; Albert, T.J.; Benz, J.; Rudolph, M.G.; Bergmann, F.; Streidl, T.; Kratzsch, P.; Boenitz-Dulat, M.; Oelschlaegel, T.; Schraeml, M.
    Discovery of a microbial transglutaminase enabling highly site-specific labeling of proteins (2017), J. Biol. Chem., 292, 15622-15635 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.2.13 synthesis the Kutzneria albida microbial transglutaminase enabling highly site-specific labeling of proteins. Its site specificity, favorable kinetics, ease of use, and cost-effective production render KalbTG an attractive tool for a broad range of applications, including production of therapeutic antibody-drug conjugates. The high activity and low molecular mass of KalbTG signifies a key advantage for mass production and enzymatic labeling purposes Kutzneria albida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 functional recombinant expression of N-terminally His-tagged soluble MTG in Escherichia coli strain Bl21 Tuner Kutzneria albida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.13 purified recombinant soluble KalbTG is crystallized at 22°C using the sitting drop vapor diffusion method by 1:1 mixing of 200 nl of 8 mg/ml protein in PBS with an unbuffered reservoir consisting of 0.2 M ammonium tartrate, and 20% PEG 3350, X-ray diffraction structure determination and analysis at 1.9 A resolution, structure modeling, the first 19 N-terminal amino acids (MGGGSTTAQAAAVAAPTPR) and the C-terminal artificial GGGSHis8 tag are disordered in the structure Kutzneria albida

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 additional information by first reacting a bifunctionalized peptide with the more specific KalbTG and in a second step with the less specific MTG from Streptomyces mobaraensis (UniProt ID P81453), a successful bio-orthogonal labeling system is demonstrated. Fusing the KalbTG recognition motif to an antibody allows for site-specific and ratio-controlled labeling using low label excess Kutzneria albida

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 ammonium a natural inhibitor of the enzyme, prevents cross-linking activity Kutzneria albida
2.3.2.13 N-(biotinyl)cadaverine
-
 Kutzneria albida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 protein glutamine + alkylamine Kutzneria albida
-
 protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine Kutzneria albida DSM 43870
-
 protein N5-alkylglutamine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Kutzneria albida W5WHY8
-
-
2.3.2.13 Kutzneria albida DSM 43870 W5WHY8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 additional information microbial transglutaminase, MTG, from Kutzneria albida (KalbTG) exhibits no cross-reactivity with known MTG substrates or commonly used target proteins, such as antibodies. The motifs YRYRQ and RYESK are the best Gln and Lys substrates of KalbTG, respectively. Substrate specificity analysis, overview Kutzneria albida ?
-
-
2.3.2.13 additional information microbial transglutaminase, MTG, from Kutzneria albida (KalbTG) exhibits no cross-reactivity with known MTG substrates or commonly used target proteins, such as antibodies. The motifs YRYRQ and RYESK are the best Gln and Lys substrates of KalbTG, respectively. Substrate specificity analysis, overview Kutzneria albida DSM 43870 ?
-
-
2.3.2.13 protein glutamine + alkylamine
-
 Kutzneria albida protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine
-
 Kutzneria albida DSM 43870 protein N5-alkylglutamine + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 KalbTG
-
 Kutzneria albida
2.3.2.13 KALB_7456
-
 Kutzneria albida
2.3.2.13 microbial transglutaminases
-
 Kutzneria albida
2.3.2.13 MTG
-
 Kutzneria albida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 37 42 assay at Kutzneria albida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 8
-
 assay at Kutzneria albida