Literature summary extracted from

Behal, R.H.; DeBuysere, M.S.; Demeler, B.; Hansen, J.C.; Olson, M.S.
Pyruvate dehydrogenase multienzyme complex. Characterization of assembly intermediates by sedimentation velocity analysis (1994), J. Biol. Chem., 269, 31372-31377 .

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.2.1.104	Guanidinium chloride	treatment of the E2-X subcomplex with 4 M guanidinium chloride causes a complete loss of enzymatic activity and the dissociation of the subcomplex into monomeric 1.5-3 S species. Removal of the chaotrope by dialysis for 18 h results in complete restoration of E2 enzymatic activity and reassembly of a 32 S subcomplex. The reassembled E2-X subcomplex demonstrates the presence of an 8 S assembly intermediate. The 8 S species associates non-cooperatively to yield additional assembly intermediates exhibiting sedimentation coefficients of 10-32 S	Bos taurus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.2.1.104	Bos taurus	-	-	-

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
1.2.1.104	treatment of the E2-X subcomplex with 4 M guanidinium chloride causes a complete loss of enzymatic activity and the dissociation of the subcomplex into monomeric 1.5-3 S species. Removal of the chaotrope by dialysis for 18 h results in complete restoration of E2 enzymatic activity and reassembly of a 32 S subcomplex	Bos taurus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.2.1.104	heart	-	Bos taurus	-

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Release 2023.1 (January 2023)