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Literature summary extracted from

  • Walden Jr., T.; Howes, N.; Farkas, W.
    Purification and properties of guanine, queuine-tRNA transglycosylase from wheat germ (1982), J. Biol. Chem., 257, 13218-13222 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.64 7-(aminomethyl)-7-deazaguanine 0.01 mM, 28% inhibition Triticum aestivum
2.4.2.64 Cd2+ 10 mM Triticum aestivum
2.4.2.64 Co2+ 10 mM Triticum aestivum
2.4.2.64 Cu2+ 10 mM Triticum aestivum
2.4.2.64 Mn2+ 10 mM Triticum aestivum
2.4.2.64 Ni2+ 10 mM Triticum aestivum
2.4.2.64 Pb2+ 10 mM Triticum aestivum
2.4.2.64 queuine
-
 Triticum aestivum
2.4.2.64 Zn2+ 10 mM Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.64 0.00006
-
 guanine pH and temperature not specified in the publication Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.64 Ba2+ 1 mM, less effective than Mg2+ in meeting the divalent ion requirement Triticum aestivum
2.4.2.64 Ca2+ 1 mM, less effective than Mg2+ in meeting the divalent ion requirement Triticum aestivum
2.4.2.64 Cs+ less effective than Na+ in meeting the monovalent ion requirement Triticum aestivum
2.4.2.64 K+ less effective than Na+ in meeting the monovalent ion requirement Triticum aestivum
2.4.2.64 Li+ less effective than Na+ in meeting the monovalent ion requirement Triticum aestivum
2.4.2.64 Mg2+ the enzyme requires a monovalent or a divalent cation. Mg2+ is most effective in meeting the divalent cation requirement. Optimal Mg2+ concentration is 5.0 mM Triticum aestivum
2.4.2.64 Na+ the enzyme requires a monovalent or a divalent cation. Na+ is most effective in meeting the monovalent requirement. Optimal concentration is 114 mM Triticum aestivum
2.4.2.64 NaCl optimum concentration is 114 mM Triticum aestivum
2.4.2.64 Rb+ less effective than Na+ in meeting the monovalent ion requirement Triticum aestivum
2.4.2.64 Sr2+ 1 mM, less effective than Mg2+ in meeting the divalent ion requirement Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.64 140000
-
 gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.64 guanine34 in tRNA + queuine Triticum aestivum
-
 queuine34 in tRNA + guanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.64 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.64
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.64 plant embryo
-
 Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.64 guanine34 in tRNA + queuine
-
 Triticum aestivum queuine34 in tRNA + guanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.64 dimer 2 * 68000, SDS-PAGE Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
2.4.2.64 guanine,queuine-tRNA transglycosylase
-
 Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.64 7.6
-
-
 Triticum aestivum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.64 0.000095
-
 queuine pH and temperature not specified in the publication Triticum aestivum