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Literature summary extracted from

  • Xu, H.T.; Hassounah, S.A.; Colby-Germinario, S.P.; Oliveira, M.; Fogarty, C.; Quan, Y.; Han, Y.; Golubkov, O.; Ibanescu, I.; Brenner, B.; Stranix, B.R.; Wainberg, M.A.
    Purification of Zika virus RNA-dependent RNA polymerase and its use to identify small-molecule Zika inhibitors (2017), J. Antimicrob. Chemother., 72, 727-734 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.48 S604T the mutation confers resistance to nucleotide inhibitors, also conferred resistance to sofosbuvir triphosphate but not to 5-azidomethyl-3-hydroxy-4-hydroxymethyl-pyridine-2-carboxylic acid hydroxyamide Zika virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 5-azidomethyl-3-hydroxy-4-hydroxymethyl-pyridine-2-carboxylic acid hydroxyamide i.e. DMB213. Competitive with natural nucleoside triphosphate substrates Zika virus
2.7.7.48 sofosbuvir triphosphate
-
 Zika virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Zika virus
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Zika virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
 Zika virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Zika virus diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 NS5 polymerase
-
 Zika virus
2.7.7.48 RDRP
-
 Zika virus
2.7.7.48 RNA-dependent RNA polymerase
-
 Zika virus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.48 0.0052
-
 30°C, pH 6.8 Zika virus 5-azidomethyl-3-hydroxy-4-hydroxymethyl-pyridine-2-carboxylic acid hydroxyamide
2.7.7.48 0.0073
-
 30°C, pH 6.8 Zika virus sofosbuvir triphosphate