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Literature summary extracted from

  • Makris, T.; Vu, V.; Meier, K.; Komor, A.; Rivard, B.; Muenck, E.; Que, L.; Lipscomb, J.
    An unusual peroxo intermediate of the arylamine oxygenase of the chloramphenicol biosynthetic pathway (2015), J. Am. Chem. Soc., 137, 1608-1617 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.67 expression in Escherichia coli Streptomyces venezuelae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.67 Iron enzyme contains a nonheme dinuclear iron cluster, with 2.1 iron atoms per monomer. Addition of O2 to the diferrous state of the cluster results in an exceptionally long-lived intermediate (t1/2 is 3 h at 4°C) that is assigned as a peroxodiferric species. CmlI-peroxo does not have a my-eta1:eta1-peroxo ligand, a my-eta1:eta2-peroxo ligand is proposed Streptomyces venezuelae

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.67 Streptomyces venezuelae F2RB83
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1.14.99.67 Streptomyces venezuelae DSM 40230 F2RB83
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2
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 Streptomyces venezuelae chloramphenicol + phenazine methosulfate + 2 H2O
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 ?
1.14.99.67 (1R,2R)-(-)-2-(N-dichloroacetyl)-amino-1-(4-aminophenyl)-1,3-propanediol + reduced phenazine methosulfate + 2 O2
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 Streptomyces venezuelae DSM 40230 chloramphenicol + phenazine methosulfate + 2 H2O
-
 ?
1.14.99.67 4-aminobenzoate + reduced phenazine methosulfate + 2 O2
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 Streptomyces venezuelae 4-nitrobenzoate + phenazine methosulfate + 2 H2O
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 ?
1.14.99.67 4-aminobenzoate + reduced phenazine methosulfate + 2 O2
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 Streptomyces venezuelae DSM 40230 4-nitrobenzoate + phenazine methosulfate + 2 H2O
-
 ?
1.14.99.67 4-aminophenol + reduced phenazine methosulfate + 2 O2
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 Streptomyces venezuelae 4-nitrophenol + phenazine methosulfate + 2 H2O
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 ?
1.14.99.67 4-aminophenol + reduced phenazine methosulfate + 2 O2
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 Streptomyces venezuelae DSM 40230 4-nitrophenol + phenazine methosulfate + 2 H2O
-
 ?
1.14.99.67 L-4-aminophenylalanine + reduced phenazine methosulfate + 2 O2
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 Streptomyces venezuelae L-4-nitrophenylalanine + phenazine methosulfate + 2 H2O
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 ?
1.14.99.67 L-4-aminophenylalanine + reduced phenazine methosulfate + 2 O2
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 Streptomyces venezuelae DSM 40230 L-4-nitrophenylalanine + phenazine methosulfate + 2 H2O
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 ?
1.14.99.67 additional information CmlI peroxo reacts with a range of arylamine substrates by an apparent second-order process. Chloramphenicol is efficiently produced from the free arylamine precursor. No substrate: L-4-aminophenylalanine Streptomyces venezuelae ?
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1.14.99.67 additional information CmlI peroxo reacts with a range of arylamine substrates by an apparent second-order process. Chloramphenicol is efficiently produced from the free arylamine precursor. No substrate: L-4-aminophenylalanine Streptomyces venezuelae DSM 40230 ?
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