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Literature summary extracted from

  • Dangerfield, T.L.; Huang, N.Z.; Johnson, K.A.
    Remdesivir is effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase (2020), iScience, 23, 101849 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 co-expression of SARS-CoV-2 RNA-dependent RNA polymerase subunits with chaperones gives soluble expression in Escherichia coli Severe acute respiratory syndrome coronavirus 2

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 remdesivir triphosphate effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase Severe acute respiratory syndrome coronavirus 2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.48 0.053
-
remdesivir triphosphate pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
2.7.7.48 0.13
-
UTP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
2.7.7.48 0.32
-
ATP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Severe acute respiratory syndrome coronavirus 2
-
diphosphate + RNAn+1
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Severe acute respiratory syndrome coronavirus 2 P0DTD1 replicase polyprotein 1ab; SARS-CoV-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48
-
Severe acute respiratory syndrome coronavirus 2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 ATP + RNAn
-
Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
?
2.7.7.48 nucleoside triphosphate + RNAn
-
Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
?
2.7.7.48 remdesivir triphosphate + RNAn remdesivir triphosphate is effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
?
2.7.7.48 UTP + RNAn
-
Severe acute respiratory syndrome coronavirus 2 diphosphate + RNAn+1
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 RDRP
-
Severe acute respiratory syndrome coronavirus 2
2.7.7.48 RNA-dependent RNA polymerase
-
Severe acute respiratory syndrome coronavirus 2

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.48 240
-
ATP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
2.7.7.48 308
-
UTP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
2.7.7.48 680
-
remdesivir triphosphate pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.48 0.00074
-
ATP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
2.7.7.48 0.00129
-
remdesivir triphosphate pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2
2.7.7.48 0.0023
-
UTP pH 7.0, 37°C Severe acute respiratory syndrome coronavirus 2