EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.48 | co-expression of SARS-CoV-2 RNA-dependent RNA polymerase subunits with chaperones gives soluble expression in Escherichia coli | Severe acute respiratory syndrome coronavirus 2 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.48 | remdesivir triphosphate | effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase | Severe acute respiratory syndrome coronavirus 2 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.48 | 0.053 | - |
remdesivir triphosphate | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 | |
2.7.7.48 | 0.13 | - |
UTP | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 | |
2.7.7.48 | 0.32 | - |
ATP | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | nucleoside triphosphate + RNAn | Severe acute respiratory syndrome coronavirus 2 | - |
diphosphate + RNAn+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.48 | Severe acute respiratory syndrome coronavirus 2 | P0DTD1 | replicase polyprotein 1ab; SARS-CoV-2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.48 | - |
Severe acute respiratory syndrome coronavirus 2 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | ATP + RNAn | - |
Severe acute respiratory syndrome coronavirus 2 | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | - |
Severe acute respiratory syndrome coronavirus 2 | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | remdesivir triphosphate + RNAn | remdesivir triphosphate is effective in combating COVID-19 because it is a better substrate than ATP for the viral RNA-dependent RNA polymerase | Severe acute respiratory syndrome coronavirus 2 | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | UTP + RNAn | - |
Severe acute respiratory syndrome coronavirus 2 | diphosphate + RNAn+1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.48 | RDRP | - |
Severe acute respiratory syndrome coronavirus 2 |
2.7.7.48 | RNA-dependent RNA polymerase | - |
Severe acute respiratory syndrome coronavirus 2 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.48 | 240 | - |
ATP | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 | |
2.7.7.48 | 308 | - |
UTP | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 | |
2.7.7.48 | 680 | - |
remdesivir triphosphate | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.48 | 0.00074 | - |
ATP | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 | |
2.7.7.48 | 0.00129 | - |
remdesivir triphosphate | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 | |
2.7.7.48 | 0.0023 | - |
UTP | pH 7.0, 37°C | Severe acute respiratory syndrome coronavirus 2 |