EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.33 | drug development | since Entamoeba histolytica PanK (EhPanK) is physiologically important and sufficiently divergent from its human orthologs, this enzyme represents an attractive target for the development of novel anti-amebic chemotherapies | Entamoeba histolytica |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.33 | gene EHI_183060, phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant expression of Myc-tagged enzyme in Entamoeba histolytica strain HM-1:IMSS cl6, recombinant expression of His-tagged enzyme from pCOLD1-EhPanK in Escherichia coli strain BL21(DE3) cells | Entamoeba histolytica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.33 | additional information | epigenetic gene silencing of PanK resulting in a significant reduction of PanK activity, intracellular CoA concentrations, and growth retardation in vitro, reinforcing the importance of this gene in Entamoeba histolytica. In Pank-silenced cells, the genes encoding other enzymes involved in CoA biosynthesis are upregulated 1.2, 2.1, 3.0, and 5.2 fold for PPCS-PPCDC, PPAT, DPCK1, and DPCK2, respectively. Growth kinetic analysis | Entamoeba histolytica |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.33 | enzyme EhPanK is robust | Entamoeba histolytica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.33 | acetyl-CoA | mixed-type inhibition versus pantothenate and ATP | Entamoeba histolytica | |
2.7.1.33 | cephaloridine | - |
Entamoeba histolytica | |
2.7.1.33 | CoA | competitive versus ATP, uncompetitive versus pantothenate | Entamoeba histolytica | |
2.7.1.33 | echinomycin | - |
Entamoeba histolytica | |
2.7.1.33 | erythromycin A | - |
Entamoeba histolytica | |
2.7.1.33 | fluorescamine | - |
Entamoeba histolytica | |
2.7.1.33 | gardimycin | - |
Entamoeba histolytica | |
2.7.1.33 | hygromycin A | - |
Entamoeba histolytica | |
2.7.1.33 | kasugamycin | - |
Entamoeba histolytica | |
2.7.1.33 | malonyl CoA | competitive versus ATP, uncompetitive versus pantothenate | Entamoeba histolytica | |
2.7.1.33 | additional information | inhibitor library screening and evaluation of cytotoxicity against human fibroblast MRC-5 cells. IC50 values for cytotoxicity against Entamoeba trophozoites and human MRC-5 cells, overview | Entamoeba histolytica | |
2.7.1.33 | neomycin | - |
Entamoeba histolytica | |
2.7.1.33 | O-methylnanaomycin A | - |
Entamoeba histolytica | |
2.7.1.33 | rosamycin | - |
Entamoeba histolytica | |
2.7.1.33 | streptomycin | - |
Entamoeba histolytica | |
2.7.1.33 | teicoplanin | - |
Entamoeba histolytica | |
2.7.1.33 | tirandamycin A | - |
Entamoeba histolytica | |
2.7.1.33 | trichostatin A | - |
Entamoeba histolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.33 | additional information | - |
additional information | Michaelis-Menten kinetics. EhPanK exhibits hyperbolic saturation kinetics when assayed over the substrate range of 0.004-0.256 mM for pantothenate in the presence of 0.025-0.10 mM ATP and 0.001-0.10 mM ATP in the presence of 0.008-0.128 mM pantothenate | Entamoeba histolytica | |
2.7.1.33 | 0.0414 | - |
ATP | pH 6.0, 37°C, recombinant enzyme | Entamoeba histolytica | |
2.7.1.33 | 0.0532 | - |
(R)-pantothenate | pH 6.0, 37°C, recombinant enzyme | Entamoeba histolytica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.33 | cytosol | - |
Entamoeba histolytica | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.33 | Ca2+ | results in 17.0% activity compard to Mg2+ | Entamoeba histolytica | |
2.7.1.33 | Co2+ | results in 74.2% activity compard to Mg2+ | Entamoeba histolytica | |
2.7.1.33 | Cu2+ | results in 84.3% activity compard to Mg2+ | Entamoeba histolytica | |
2.7.1.33 | Fe2+ | results in 72.8% activity compard to Mg2+ | Entamoeba histolytica | |
2.7.1.33 | Li+ | results in 34.5% activity compard to Mg2+ | Entamoeba histolytica | |
2.7.1.33 | Mg2+ | reuqired | Entamoeba histolytica | |
2.7.1.33 | Mg2+ | best cation | Entamoeba histolytica | |
2.7.1.33 | Mn2+ | results in 83.2% activity compard to Mg2+ | Entamoeba histolytica | |
2.7.1.33 | additional information | EhPanK shows an absolute requirement for a free bivalent metal cofactor, with Mg2+ as the preferred cation. No activity with Ni2+, Na+, and K+ | Entamoeba histolytica | |
2.7.1.33 | Zn2+ | results in 3.9% activity compard to Mg2+ | Entamoeba histolytica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.33 | ATP + (R)-pantothenate | Entamoeba histolytica | - |
ADP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | CTP + (R)-pantothenate | Entamoeba histolytica | - |
CDP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | dATP + (R)-pantothenate | Entamoeba histolytica | - |
dADP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | GTP + (R)-pantothenate | Entamoeba histolytica | - |
GDP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | polyphosphate(n) + (R)-pantothenate | Entamoeba histolytica | - |
polyphosphate(n-1) + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | UTP + (R)-pantothenate | Entamoeba histolytica | - |
UDP + (R)-4'-phosphopantothenate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.33 | Entamoeba histolytica | B1N2P3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.33 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) cells by nickel affinity chromatography | Entamoeba histolytica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.33 | trophozoite | - |
Entamoeba histolytica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.33 | ATP + (R)-pantothenate | - |
Entamoeba histolytica | ADP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | CTP + (R)-pantothenate | - |
Entamoeba histolytica | CDP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | dATP + (R)-pantothenate | - |
Entamoeba histolytica | dADP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | GTP + (R)-pantothenate | - |
Entamoeba histolytica | GDP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | additional information | EhPanK utilizes various nucleoside triphosphates, ATP, CTP, GTP, UTP, dATP, and polyphosphates, as well as deoxynucleotides as phosphoryl donors, with ATP being the best phosphate donor | Entamoeba histolytica | ? | - |
- |
|
2.7.1.33 | polyphosphate(n) + (R)-pantothenate | - |
Entamoeba histolytica | polyphosphate(n-1) + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | UTP + (R)-pantothenate | - |
Entamoeba histolytica | UDP + (R)-4'-phosphopantothenate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.33 | EhPAnK | - |
Entamoeba histolytica |
2.7.1.33 | PanK | - |
Entamoeba histolytica |
2.7.1.33 | pantothenate kinase 1 | - |
Entamoeba histolytica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.33 | 37 | - |
- |
Entamoeba histolytica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.33 | 6 | - |
enzyme EhPanK is catalytically active in broad pH range | Entamoeba histolytica |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.1.33 | 0.0263 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | teicoplanin | |
2.7.1.33 | 0.0492 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | gardimycin | |
2.7.1.33 | 0.0778 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | echinomycin | |
2.7.1.33 | 0.1313 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | erythromycin A | |
2.7.1.33 | 0.1472 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | neomycin | |
2.7.1.33 | 0.1554 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | streptomycin | |
2.7.1.33 | 0.1657 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | rosamycin | |
2.7.1.33 | 0.1937 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | hygromycin A | |
2.7.1.33 | 0.2288 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | tirandamycin A | |
2.7.1.33 | 0.2299 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | cephaloridine | |
2.7.1.33 | 0.2541 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | kasugamycin | |
2.7.1.33 | 0.2838 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | O-methylnanaomycin A | |
2.7.1.33 | 0.3099 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | trichostatin A | |
2.7.1.33 | 0.3464 | - |
recombinant enzyme, pH 6.0, 37°C | Entamoeba histolytica | fluorescamine |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.33 | malfunction | epigenetic gene silencing of PanK resulting in a significant reduction of PanK activity, intracellular CoA concentrations, and growth retardation in vitro, reinforcing the importance of this gene in Entamoeba histolytica | Entamoeba histolytica |
2.7.1.33 | metabolism | four key enzymes are involved in the CoA pathway: pantothenate kinase (PanK, EC 2.7.1.33), bifunctional phosphopantothenate-cysteine ligase/decarboxylase (PPCS-PPCDC), phosphopantetheine adenylyltransferase (PPAT) and dephospho-CoA kinase (DPCK) | Entamoeba histolytica |