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Literature summary extracted from

  • Osman, W.H.W.; Mikami, B.; Saka, N.; Kondo, K.; Lin, M.I.; Nagata, T.; Katahira, M.
    Identification of key residues for activities of atypical glutathione S-transferase of Ceriporiopsis subvermispora, a selective degrader of lignin in woody biomass, by crystallography and functional mutagenesis (2019), Int. J. Biol. Macromol., 132, 222-229 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.18 enzyme in GSH-free and -bound forms, using 20% (w/v) PEG8000, 100 mM HEPES-HCl (pH 7.5), 200 mM ammonium sulfate, and 20% (v/v) 2-propanol at 4°C Gelatoporia subvermispora

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 N22A the mutant shows reduced activity towards 1-chloro-2,4-dinitrobenzene and increased activity towards 5-chloromethylfluorescein diacetate compared to the wild type enzyme Gelatoporia subvermispora
2.5.1.18 N24A the mutant shows no activity towards 1-chloro-2,4-dinitrobenzene and alpha-O-(beta-methylumbelliferyl)acetovanillone and increased activity towards 5-chloromethylfluorescein diacetate and cumene hydroperoxide compared to the wild type enzyme Gelatoporia subvermispora
2.5.1.18 Y46A the mutant shows no activity towards 1-chloro-2,4-dinitrobenzene and increased activity towards 5-chloromethylfluorescein diacetate compared to the wild type enzyme Gelatoporia subvermispora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.00003
-
 4-methylumbelliferyl acetate mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.00022
-
 5-chloromethylfluorescein diacetate mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.00033
-
 5-chloromethylfluorescein diacetate mutant enzyme N22A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.00049
-
 5-chloromethylfluorescein diacetate mutant enzyme Y46A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.00094
-
 4-methylumbelliferyl acetate wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.01162
-
 5-chloromethylfluorescein diacetate wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.01461
-
 alpha-O-(beta-methylumbelliferyl)acetovanillone wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.11
-
 cumene hydroperoxide wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.412
-
 1-chloro-2,4-dinitrobenzene mutant enzyme N22A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.602
-
 1-chloro-2,4-dinitrobenzene wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 5.3 6 cumene hydroperoxide mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Gelatoporia subvermispora M2QJS8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 anion exchange chromatography and Superdex S200 gel filtration Gelatoporia subvermispora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Gelatoporia subvermispora chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 glutathione + 4-methylumbelliferyl acetate
-
 Gelatoporia subvermispora ?
-
 ?
2.5.1.18 glutathione + 5-chloromethylfluorescein diacetate
-
 Gelatoporia subvermispora ?
-
 ?
2.5.1.18 glutathione + alpha-O-(beta-methylumbelliferyl)acetovanillone
-
 Gelatoporia subvermispora ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Gelatoporia subvermispora ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 homodimer 2 * 31000, SDS-PAGE Gelatoporia subvermispora
2.5.1.18 homodimer 2 * 29830, calculated from amino acid sequence Gelatoporia subvermispora

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Gelatoporia subvermispora
2.5.1.18 GST83044
-
 Gelatoporia subvermispora

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.000000451
-
 alpha-O-(beta-methylumbelliferyl)acetovanillone wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.00000163
-
 4-methylumbelliferyl acetate mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.000146
-
 5-chloromethylfluorescein diacetate mutant enzyme Y46A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.000321
-
 5-chloromethylfluorescein diacetate mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.0007
-
 cumene hydroperoxide mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.000714
-
 5-chloromethylfluorescein diacetate wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.000906
-
 5-chloromethylfluorescein diacetate mutant enzyme N22A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.00333
-
 4-methylumbelliferyl acetate wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.0678
-
 1-chloro-2,4-dinitrobenzene mutant enzyme N22A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.18
-
 1-chloro-2,4-dinitrobenzene wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.21
-
 cumene hydroperoxide wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.0000309
-
 alpha-O-(beta-methylumbelliferyl)acetovanillone wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.05433
-
 4-methylumbelliferyl acetate mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.06145
-
 5-chloromethylfluorescein diacetate wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.131
-
 cumene hydroperoxide mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.165
-
 1-chloro-2,4-dinitrobenzene mutant enzyme N22A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.298
-
 5-chloromethylfluorescein diacetate mutant enzyme Y46A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 0.299
-
 1-chloro-2,4-dinitrobenzene wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 1.46
-
 5-chloromethylfluorescein diacetate mutant enzyme N24A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 1.91
-
 cumene hydroperoxide wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 2.75
-
 5-chloromethylfluorescein diacetate mutant enzyme N22A, pH and temperature not specified in the publication Gelatoporia subvermispora
2.5.1.18 3.54
-
 4-methylumbelliferyl acetate wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora