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Literature summary extracted from

  • Zhang, J.; Zhou, Y.; Liu, K.; Chu, J.; Zhang, Y.; He, B.
    Ca2+-induced stabilization of the nucleoside 2'-deoxyribosyltransferase from Lactobacillus hilgardii ZJS01 Characteristics and application in nucleosides synthesis (2018), Int. J. Biol. Macromol., 106, 963-968 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.6 synthesis compared with NPTs (EC 2.4.2.5), NDTs present the advantage of catalyzing transglycosylation reactions between purine or pyrimidine bases and nucleosides in one enzyme one-pot mode Lentilactobacillus hilgardii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.6 gene ndt, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Lentilactobacillus hilgardii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.6 EDTA strong inhibition Lentilactobacillus hilgardii
2.4.2.6 Mg2+ strong inhibition Lentilactobacillus hilgardii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.6 Ca2+ stabilizes the enzyme 1t 10-20 mM, Ca2+ markedly improves the thermostability of LhNDT, significantly stimulates the LhNDT activity up to 184% at 10 mM Lentilactobacillus hilgardii
2.4.2.6 Co2+ significantly stimulates the LhNDT activity up to 146% at 10 mM Lentilactobacillus hilgardii
2.4.2.6 Mn2+ significantly stimulates the LhNDT activity up to 127% at 10 mM Lentilactobacillus hilgardii
2.4.2.6 additional information no significant changes are observed with Ba2+ and Cr3+ Lentilactobacillus hilgardii
2.4.2.6 Ni2+ significantly stimulates the LhNDT activity up to 138% at 10 mM Lentilactobacillus hilgardii
2.4.2.6 Zn2+ significantly stimulates the LhNDT activity up to 124% at 10 mM Lentilactobacillus hilgardii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.6 22000
-
 recombinant His-tagged enzyme, gel filtration Lentilactobacillus hilgardii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Lentilactobacillus hilgardii
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Lentilactobacillus hilgardii ZJS01
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.6 Lentilactobacillus hilgardii A0A286R847
-
-
2.4.2.6 Lentilactobacillus hilgardii ZJS01 A0A286R847
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Lentilactobacillus hilgardii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.6 2'-deoxyuridine + 2,6-diaminopurine
-
 Lentilactobacillus hilgardii 2,6-diaminopurine-2'-deoxyriboside + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + 2,6-diaminopurine
-
 Lentilactobacillus hilgardii ZJS01 2,6-diaminopurine-2'-deoxyriboside + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + 5-azacytosine
-
 Lentilactobacillus hilgardii 5-aza-2'-deoxycytidine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + 5-azacytosine
-
 Lentilactobacillus hilgardii ZJS01 5-aza-2'-deoxycytidine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + 5-fluorocytosine
-
 Lentilactobacillus hilgardii 5-fluoro-2'-deoxycytidine + uracil
-
 ?
2.4.2.6 2'-deoxyuridine + 5-fluorocytosine
-
 Lentilactobacillus hilgardii ZJS01 5-fluoro-2'-deoxycytidine + uracil
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Lentilactobacillus hilgardii 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Lentilactobacillus hilgardii ZJS01 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 additional information NDT can directly transfer 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor. LhNDT does not only catalyze the transfer between purines but also the transfer between pyrimidines Lentilactobacillus hilgardii ?
-
-
2.4.2.6 additional information NDT can directly transfer 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor. LhNDT does not only catalyze the transfer between purines but also the transfer between pyrimidines Lentilactobacillus hilgardii ZJS01 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.4.2.6 monomer 1 * 21000, recombinant His-tagged enzyme, SDS-PAGE and gel filtration Lentilactobacillus hilgardii
2.4.2.6 More LhNDT is a monomer as revealed by gel filtration with or without addition of Ca2+ in elution buffer Lentilactobacillus hilgardii

Synonyms

EC Number Synonyms Comment Organism
2.4.2.6 GQR93_14380 locus name Lentilactobacillus hilgardii
2.4.2.6 LhNDT
-
 Lentilactobacillus hilgardii
2.4.2.6 nucleoside 2'-deoxyribosyltransferase
-
 Lentilactobacillus hilgardii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.6 35 40 assay at Lentilactobacillus hilgardii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.6 additional information
-
 LhNDT is thermosensitive. Ca2+ markedly improves the thermostability of LhNDT. The enzyme activity decreases sharply when no Ca2+ is added and is completely lost within 6 h at 35°C. Such inactivity is reversed significantly with the addition of Ca2+ at concentrations ranging from 5 mM to 20 mM, and 80 % of the initial activity could be maintained for at least 12 h at a concentration higher than 10 mM. Stability is most significantly enhanced in the presence of 15 mM Ca2+ (the specific concentration of Ca2+ is 0.106 mmol/mg). Lentilactobacillus hilgardii
2.4.2.6 30 35 with the addition of 15 mM Ca2+, the thermostability of LhNDT is greatly promoted by 68 and 47 times at 30°C and 35°C, respectively. LhNDT retains approx. 40% activity after 1 h at 35°C Lentilactobacillus hilgardii
2.4.2.6 60
-
 half-life of the free LrNDT is 17.9 min Lentilactobacillus hilgardii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.6 5
-
 assay at Lentilactobacillus hilgardii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.6 5 7.4 purified recombinant LhNDT retains more than 90% of its original activity between after 1 h Lentilactobacillus hilgardii

General Information

EC Number General Information Comment Organism
2.4.2.6 evolution NDTs are classified into two classes depending on their substrate specificity: NDT type I (PDT), specific for purine exchange, and NDT type II (NDT), which catalyzes the transfer between purines and/or pyrimidines. LhNDT is classified as a NDT type II Lentilactobacillus hilgardii