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Literature summary extracted from

  • Planchestainer, M.; Contente, M.; Cassidy, J.; Molinari, F.; Tamborini, L.; Paradisi, F.
    Continuous flow biocatalysis Production and in-line purification of amines by immobilised transaminase from Halomonas elongata (2017), Green Chem., 19, 372-375 .
No PubMed abstract available

Application

EC Number Application Comment Organism
2.6.1.B16 synthesis the covalently immobilized enzyme is used for amine bioproduction in batch and flow reaction systems, method evaluation, overview. Produced vanillylamine is a valuable building block for the synthesis of natural products, such as capsaicinoids Halomonas elongata

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.B16 additional information development of an efficient bioproduction of amines by exploiting the immobilised (S)-selective amine transaminase from Halomonas elongata (HEWT), which shows a very broad substrate scope, tolerating a range of temperatures, pH values, salts, and co-solvents, in a continuous flow-reactor. The immobilised HEWT (imm-HEWT) is applied to continuous-flow reactions maintaining the biocatalyst in a packed-bed reactor to decrease reaction times and increase the productivity. The addition of an in-line purification step allows for the recovery of the pure products without any additional work-up procedure. Method optimization and evaluation, overview Halomonas elongata

General Stability

EC Number General Stability Organism
2.6.1.B16 the HEWT quaternary structure is quite unstable Halomonas elongata

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.B16 Halomonas elongata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.B16 (S)-(-)-1-phenyl-ethylamine + 4-nitrobenzaldehyde complete conversion at pH 8.0 and 37°C Halomonas elongata 4-nitrobenzylamine + L-alanine
-
 ?
2.6.1.B16 (S)-(-)-1-phenyl-ethylamine + cinnamaldehyde
-
 Halomonas elongata cinnamylamine + L-alanine
-
 ?
2.6.1.B16 (S)-(-)-1-phenyl-ethylamine + vanillin
-
 Halomonas elongata vanillylamine + L-alanine
-
 ?
2.6.1.B16 (S)-1-phenylethylamine + pyruvate
-
 Halomonas elongata L-alanine + acetophenone
-
 r
2.6.1.B16 additional information vanillylamine is a valuable building block for the synthesis of natural products, such as capsaicinoids Halomonas elongata ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.6.1.B16 (S)-selective amine transaminase
-
 Halomonas elongata
2.6.1.B16 HEWT
-
 Halomonas elongata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.B16 37
-
 assay at Halomonas elongata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.B16 8
-
 assay at Halomonas elongata

General Information

EC Number General Information Comment Organism
2.6.1.B16 physiological function transaminases play a central role in the biocatalytic preparation of enantiopure amines and amino acids, challenging reactions to achieve by conventional synthesis Halomonas elongata