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Literature summary extracted from

  • Ramirez, A.S.; Boilevin, J.; Lin, C.W.; Ha Gan, B.; Janser, D.; Aebi, M.; Darbre, T.; Reymond, J.L.; Locher, K.P.
    Chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins (2017), Glycobiology, 27, 726-733 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.131 gene ALG11, recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) Saccharomyces cerevisiae
2.4.1.142 gene ALG1, recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) Saccharomyces cerevisiae
2.4.1.257 gene ALG2, transient recombinant expression of N-terminally His10-YFP tagged enzyme in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.131 additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1 (from yeast, EC 2.4.1.142), Alg2 (from human, EC 2.4.1.257), and Alg11 proteins, chemo-enzymatic synthesis strategy to extend the glycan moiety of synthetic LLO analogues to Dol-PP-GlcNAc2Man5, method, overview Saccharomyces cerevisiae
2.4.1.142 additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 (from human, EC 2.4.1.257), and Alg11 (from yeast, EC 2.4.1.131) proteins, chemo-enzymatic synthesis strategy to extend the glycan moiety of synthetic LLO analogues to Dol-PP-GlcNAc2Man5, method, overview Saccharomyces cerevisiae
2.4.1.257 additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1 (from yeast, EC 2.4.1.142), Alg2, and Alg11 (from yeast, EC 2.4.1.131) proteins, chemo-enzymatic synthesis strategy to extend the glycan moiety of synthetic LLO analogues to Dol-PP-GlcNAc2Man5, method, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.257 endoplasmic reticulum
-
 Homo sapiens 5783
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.131 2 GDP-alpha-D-mannose + alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol Saccharomyces cerevisiae
-
 2 GDP + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 ?
2.4.1.131 2 GDP-alpha-D-mannose + alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol Saccharomyces cerevisiae ATCC 204508
-
 2 GDP + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 ?
2.4.1.142 GDP-alpha-D-mannose + N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphodolichol Saccharomyces cerevisiae
-
 GDP + beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?
2.4.1.142 GDP-alpha-D-mannose + N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphodolichol Saccharomyces cerevisiae ATCC 204508
-
 GDP + beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?
2.4.1.257 GDP-alpha-D-mannose + alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol Homo sapiens
-
 GDP + alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.131 Saccharomyces cerevisiae P53954
-
-
2.4.1.131 Saccharomyces cerevisiae ATCC 204508 P53954
-
-
2.4.1.142 Saccharomyces cerevisiae P16661
-
-
2.4.1.142 Saccharomyces cerevisiae ATCC 204508 P16661
-
-
2.4.1.257 Homo sapiens Q9H553
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.131 recombinant N-terminally His10-tagged enzyme ALG11 from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration Saccharomyces cerevisiae
2.4.1.142 recombinant N-terminally His10-tagged enzyme ALG1 from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration Saccharomyces cerevisiae
2.4.1.257 recombinant N-terminally His10-YFP tagged enzyme from HEK-293 cells by solubilization from membranes with detergent, nickel affinity chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.131 2 GDP-alpha-D-mannose + alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 Saccharomyces cerevisiae 2 GDP + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 ?
2.4.1.131 2 GDP-alpha-D-mannose + alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 Saccharomyces cerevisiae ATCC 204508 2 GDP + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 ?
2.4.1.131 additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins. Comparison to the reaction of dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3A (EC 2.4.99.18) Saccharomyces cerevisiae ?
-
-
2.4.1.131 additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins. Comparison to the reaction of dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3A (EC 2.4.99.18) Saccharomyces cerevisiae ATCC 204508 ?
-
-
2.4.1.142 GDP-alpha-D-mannose + N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 Saccharomyces cerevisiae GDP + beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?
2.4.1.142 GDP-alpha-D-mannose + N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 Saccharomyces cerevisiae ATCC 204508 GDP + beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?
2.4.1.142 additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins. Comparison to the reaction of dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3A (EC 2.4.99.18) Saccharomyces cerevisiae ?
-
-
2.4.1.142 additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins. Comparison to the reaction of dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3A (EC 2.4.99.18) Saccharomyces cerevisiae ATCC 204508 ?
-
-
2.4.1.257 GDP-alpha-D-mannose + alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 Homo sapiens GDP + alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
 ?
2.4.1.257 additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins. Comparison to the reaction of dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3A (EC 2.4.99.18) Homo sapiens ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.131 Alg11
-
 Saccharomyces cerevisiae
2.4.1.142 ALG1
-
 Saccharomyces cerevisiae
2.4.1.257 Alg2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.131 25
-
 assay at Saccharomyces cerevisiae
2.4.1.142 37
-
 assay at Saccharomyces cerevisiae
2.4.1.257 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.131 7.5
-
 assay at Saccharomyces cerevisiae
2.4.1.142 7.5
-
 assay at Saccharomyces cerevisiae
2.4.1.257 7.5
-
 assay at Homo sapiens