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Literature summary extracted from

  • Yang, X.; Wei, J.; Wu, Z.; Gao, J.
    Effects of substrate-binding site residues on the biochemical properties of a tau class glutathione S-transferase from Oryza sativa (2019), Genes (Basel), 11, 25 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 E68A the mutant retains activity toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole, 1-chloro-2,4-dinitrobenzene and 4-nitrobenzyl chloride, but its activities drop significantly compared to the wild type enzyme Oryza sativa
2.5.1.18 F116A the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme Oryza sativa
2.5.1.18 F162A the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme Oryza sativa
2.5.1.18 K42A the mutant shows 10% of wild type activity Oryza sativa
2.5.1.18 L112A the mutant shows reduced catalytic efficiency toward glutathione and increased catalytic efficiency toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme Oryza sativa
2.5.1.18 M17A the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme Oryza sativa
2.5.1.18 N109A the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme Oryza sativa
2.5.1.18 S15A inactive Oryza sativa
2.5.1.18 S69A the mutant loses activity to 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole while it retains 31% of the activity toward 1-chloro-2,4-dinitrobenzene and 7-8% of the activity toward 4-nitrobenzyl chloride, respectively, compared with the wild type enzyme Oryza sativa
2.5.1.18 V56A the mutant loses activity to 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole while it retains 21% of the activity toward 1-chloro-2,4-dinitrobenzene and 7-8% of the activity toward 4-nitrobenzyl chloride, respectively, compared with the wild type enzyme Oryza sativa
2.5.1.18 W161A the mutant shows reduced catalytic efficiency toward glutathione and increased catalytic efficiency toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme Oryza sativa
2.5.1.18 Y113A the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.058
-
 glutathione wild type enzyme, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.12
-
 glutathione mutant enzyme Y113A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.166
-
 glutathione mutant enzyme F116A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.192
-
 glutathione mutant enzyme N109A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.221
-
 glutathione mutant enzyme W161A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.307
-
 glutathione mutant enzyme L112A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.324
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole wild type enzyme, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.36
-
 glutathione mutant enzyme F162A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.47 1 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme Y113A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.743
-
 glutathione mutant enzyme M17A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.781
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme N109A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.882
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme F116A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.117
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme W161A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.487
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme L112A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 3.661
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme M17A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 3.866
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme F162A, at 25°C, pH not specified in the publication Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Oryza sativa Q93WY5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 Ni-Sepharose column chromatography Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Oryza sativa chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 glutathione + 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole
-
 Oryza sativa ?
-
 ?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
 Oryza sativa chloride + 4-nitrobenzyl-glutathione
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Oryza sativa ?
-
 ?
2.5.1.18 additional information no activity toward 1,2-dichloro-4-nitrobenzene, 4-nitrophenyl acetate and ethacrynic acid Oryza sativa ?
-
-

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 25000, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST
-
 Oryza sativa
2.5.1.18 GSTU17 isoform Oryza sativa
2.5.1.18 tau class glutathione S-transferase
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 40
-
-
 Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 25 55 the enzyme retains about 85, 99, 95 and 65% activity after 15 min at 25, 35, 45 and 55°C, respectively. The enzyme is inactivated at 65°C Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.196
-
 glutathione mutant enzyme N109A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.22
-
 glutathione mutant enzyme Y113A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.257
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole wild type enzyme, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.264
-
 glutathione wild type enzyme, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.266
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme N109A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.277
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme Y113A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.322
-
 glutathione mutant enzyme F116A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.387
-
 glutathione mutant enzyme M17A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.421
-
 glutathione mutant enzyme F162A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.512
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme F116A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.573
-
 glutathione mutant enzyme W161A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.809
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme M17A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.946
-
 glutathione mutant enzyme L112A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.999
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme W161A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.573
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme F162A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.75
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme L112A, at 25°C, pH not specified in the publication Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.221
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme M17A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.341
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme N109A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.407
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme F162A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.521
-
 glutathione mutant enzyme M17A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.581
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme F116A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.589
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme Y113A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.793
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole wild type enzyme, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 0.894
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme W161A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.019
-
 glutathione mutant enzyme N109A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.17
-
 glutathione mutant enzyme F162A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.177
-
 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole mutant enzyme L112A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.579
-
 glutathione mutant enzyme L112A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.842
-
 glutathione mutant enzyme Y113A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 1.942
-
 glutathione mutant enzyme F116A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 2.588
-
 glutathione mutant enzyme W161A, at 25°C, pH not specified in the publication Oryza sativa
2.5.1.18 4.552
-
 glutathione wild type enzyme, at 25°C, pH not specified in the publication Oryza sativa