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Literature summary extracted from

  • Zhuge, X.L.; Xu, H.; Xiu, Z.J.; Yang, H.L.
    Biochemical functions of glutathione S-transferase family of Salix babylonica (2020), Front. Plant Sci., 11, 364 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli BL21 (DE3) cells Salix babylonica

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 I55T the mutant of isoform GSTU6 shows strongly decreased activity compared to the wild type enzyme Salix babylonica
2.5.1.18 T53I the mutant of isoform GSTU7 shows strongly increased activity compared to the wild type enzyme Salix babylonica
2.5.1.18 T53V the mutant of isoform GSTU7 shows strongly increased activity compared to the wild type enzyme Salix babylonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.14
-
 glutathione isoform GSTU3, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.141
-
 glutathione isoform GSTU6, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.146
-
 glutathione isoform GSTU4, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.156
-
 1-chloro-2,4-dinitrobenzene isoform GSTU10, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.201
-
 1-chloro-2,4-dinitrobenzene isoform GSTU1, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.208
-
 glutathione isoform GSTU9, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.24
-
 glutathione isoform GSTU5, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.262
-
 glutathione isoform GSTU13, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.284
-
 glutathione isoform GSTU10, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.333
-
 glutathione isoform GSTU12, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.473
-
 glutathione isoform GSTU1, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.491
-
 glutathione isoform GSTU14, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.732
-
 glutathione isoform GSTU11, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.893
-
 glutathione isoform GSTU8, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.996
-
 glutathione isoform GSTU2, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.003
-
 1-chloro-2,4-dinitrobenzene isoform GSTU3, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.183
-
 glutathione isoform GSTU7, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.273
-
 1-chloro-2,4-dinitrobenzene isoform GSTU13, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.074
-
 1-chloro-2,4-dinitrobenzene isoform GSTU7, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.144
-
 1-chloro-2,4-dinitrobenzene isoform GSTU12, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.266
-
 1-chloro-2,4-dinitrobenzene isoform GSTU6, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.364
-
 1-chloro-2,4-dinitrobenzene isoform GSTU5, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.628
-
 1-chloro-2,4-dinitrobenzene isoform GSTU8, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.785
-
 1-chloro-2,4-dinitrobenzene isoform GSTU9, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 3.372
-
 1-chloro-2,4-dinitrobenzene isoform GSTU11, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 4.269
-
 1-chloro-2,4-dinitrobenzene isoform GSTU14, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 4.819
-
 1-chloro-2,4-dinitrobenzene isoform GSTU2, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 5.121
-
 1-chloro-2,4-dinitrobenzene isoform GSTU4, at pH 6.5 and 25°C Salix babylonica

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Salix babylonica A0A4Y5QZW9
-
-
2.5.1.18 Salix babylonica A0A4Y5QZX0
-
-
2.5.1.18 Salix babylonica A0A4Y5QZX2
-
-
2.5.1.18 Salix babylonica A0A4Y5QZX3
-
-
2.5.1.18 Salix babylonica A0A4Y5QZX9
-
-
2.5.1.18 Salix babylonica A0A4Y5QZZ8
-
-
2.5.1.18 Salix babylonica A0A4Y5R032
-
-
2.5.1.18 Salix babylonica A0A4Y5R087
-
-
2.5.1.18 Salix babylonica A0A4Y5R0B2
-
-
2.5.1.18 Salix babylonica A0A4Y5R0C1
-
-
2.5.1.18 Salix babylonica A0A4Y5R0K8
-
-
2.5.1.18 Salix babylonica A0A4Y5R0L5
-
-
2.5.1.18 Salix babylonica A0A4Y5R2M7
-
-
2.5.1.18 Salix babylonica A0A4Y5R2N2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 nickel-Sepharose column chromatography Salix babylonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 bud
-
 Salix babylonica
-
2.5.1.18 flower
-
 Salix babylonica
-
2.5.1.18 flower highest expression Salix babylonica
-
2.5.1.18 flower high expression Salix babylonica
-
2.5.1.18 leaf
-
 Salix babylonica
-
2.5.1.18 leaf highest expression Salix babylonica
-
2.5.1.18 phloem
-
 Salix babylonica
-
2.5.1.18 phloem high expression Salix babylonica
-
2.5.1.18 root
-
 Salix babylonica
-
2.5.1.18 root highest expression Salix babylonica
-
2.5.1.18 root high expression Salix babylonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dichloro-4-nitrobenzene
-
 Salix babylonica ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Salix babylonica chloride + 2,4-dinitrophenyl-glutathione
-
 ?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
 Salix babylonica chloride + 4-nitrobenzyl-glutathione
-
 ?
2.5.1.18 glutathione + 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole
-
 Salix babylonica ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Salix babylonica ?
-
 ?
2.5.1.18 glutathione + fluorodifen
-
 Salix babylonica ?
-
 ?
2.5.1.18 additional information no activity with 1,2-dichloro-4-nitrobenzene, dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide Salix babylonica ?
-
-
2.5.1.18 additional information no activity with dehydroascorbic acid and cumene hydroperoxide Salix babylonica ?
-
-
2.5.1.18 additional information no activity with dehydroascorbic acid, 1,2-dichloro-4-nitrobenzene and cumene hydroperoxide Salix babylonica ?
-
-
2.5.1.18 additional information no activity with dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide Salix babylonica ?
-
-
2.5.1.18 additional information no activity with dehydroascorbic acid, 4-nitrobenzyl chloride, 1,2-dichloro-4-nitrobenzene and cumene hydroperoxide Salix babylonica ?
-
-
2.5.1.18 additional information no activity with fluorodifen, 1,2-dichloro-4-nitrobenzene, dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide Salix babylonica ?
-
-

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 26100, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 24756, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 24893, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25014, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25172, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25262, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25300, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25340, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25401, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25493, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25536, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25567, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 25680, calculated from amino acid sequence Salix babylonica
2.5.1.18 ? x * 26434, calculated from amino acid sequence Salix babylonica

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Salix babylonica
2.5.1.18 GST
-
 Salix babylonica
2.5.1.18 GSTU1 isoform Salix babylonica
2.5.1.18 GSTU10 isoform Salix babylonica
2.5.1.18 GSTU11 isoform Salix babylonica
2.5.1.18 GSTU12 isoform Salix babylonica
2.5.1.18 GSTU13 isoform Salix babylonica
2.5.1.18 GSTU14 isoform Salix babylonica
2.5.1.18 GSTU2 isoform Salix babylonica
2.5.1.18 GSTU3 isoform Salix babylonica
2.5.1.18 GSTU4 isoform Salix babylonica
2.5.1.18 GSTU5 isoform Salix babylonica
2.5.1.18 GSTU6 isoform Salix babylonica
2.5.1.18 GSTU7 isoform Salix babylonica
2.5.1.18 GSTU8 isoform Salix babylonica
2.5.1.18 GSTU9 isoform Salix babylonica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.804
-
 glutathione isoform GSTU7, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.197
-
 glutathione isoform GSTU8, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.314
-
 glutathione isoform GSTU12, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.415
-
 1-chloro-2,4-dinitrobenzene isoform GSTU7, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.934
-
 1-chloro-2,4-dinitrobenzene isoform GSTU12, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.061
-
 1-chloro-2,4-dinitrobenzene isoform GSTU8, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.496
-
 glutathione isoform GSTU2, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.963
-
 glutathione isoform GSTU4, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 3.376
-
 glutathione isoform GSTU5, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 6.056
-
 1-chloro-2,4-dinitrobenzene isoform GSTU1, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 6.283
-
 glutathione isoform GSTU1, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 7.391
-
 glutathione isoform GSTU10, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 7.564
-
 glutathione isoform GSTU9, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 7.782
-
 1-chloro-2,4-dinitrobenzene isoform GSTU5, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 11.4
-
 glutathione isoform GSTU13, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 12.281
-
 glutathione isoform GSTU14, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 14.948
-
 1-chloro-2,4-dinitrobenzene isoform GSTU13, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 16.183
-
 1-chloro-2,4-dinitrobenzene isoform GSTU10, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 16.302
-
 glutathione isoform GSTU11, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 16.483
-
 glutathione isoform GSTU6, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 16.714
-
 glutathione isoform GSTU3, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 21.06
-
 1-chloro-2,4-dinitrobenzene isoform GSTU9, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 22.472
-
 1-chloro-2,4-dinitrobenzene isoform GSTU11, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 24.482
-
 1-chloro-2,4-dinitrobenzene isoform GSTU4, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 28.819
-
 1-chloro-2,4-dinitrobenzene isoform GSTU3, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 41.823
-
 1-chloro-2,4-dinitrobenzene isoform GSTU14, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 112.987
-
 1-chloro-2,4-dinitrobenzene isoform GSTU6, at pH 6.5 and 25°C Salix babylonica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 5.1
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 5.2
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 5.3
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 5.5
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 5.8
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 5.9
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 6.2
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 6.4
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 6.6
2.5.1.18 Salix babylonica calculated from amino acid sequence
-
 6.9

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.679
-
 glutathione isoform GSTU7, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.682
-
 1-chloro-2,4-dinitrobenzene isoform GSTU7, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.784
-
 1-chloro-2,4-dinitrobenzene isoform GSTU8, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 0.902
-
 1-chloro-2,4-dinitrobenzene isoform GSTU12, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.325
-
 1-chloro-2,4-dinitrobenzene isoform GSTU2, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 1.34
-
 glutathione isoform GSTU8, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 2.506
-
 glutathione isoform GSTU2, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 3.292
-
 1-chloro-2,4-dinitrobenzene isoform GSTU5, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 3.947
-
 glutathione isoform GSTU12, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 4.781
-
 1-chloro-2,4-dinitrobenzene isoform GSTU4, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 6.664
-
 1-chloro-2,4-dinitrobenzene isoform GSTU11, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 7.562
-
 1-chloro-2,4-dinitrobenzene isoform GSTU9, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 9.797
-
 1-chloro-2,4-dinitrobenzene isoform GSTU14, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 11.472
-
 1-chloro-2,4-dinitrobenzene isoform GSTU13, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 13.277
-
 glutathione isoform GSTU1, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 14.073
-
 glutathione isoform GSTU5, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 20.296
-
 glutathione isoform GSTU4, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 22.285
-
 glutathione isoform GSTU11, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 25.022
-
 glutathione isoform GSTU14, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 26.053
-
 glutathione isoform GSTU10, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 28.733
-
 1-chloro-2,4-dinitrobenzene isoform GSTU3, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 30.102
-
 1-chloro-2,4-dinitrobenzene isoform GSTU1, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 36.365
-
 glutathione isoform GSTU9, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 43.529
-
 glutathione isoform GSTU13, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 49.862
-
 1-chloro-2,4-dinitrobenzene isoform GSTU6, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 116.572
-
 glutathione isoform GSTU6, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 119.3
-
 glutathione isoform GSTU3, at pH 6.5 and 25°C Salix babylonica
2.5.1.18 7104
-
 1-chloro-2,4-dinitrobenzene isoform GSTU10, at pH 6.5 and 25°C Salix babylonica