EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.2.5 | synthesis | the enzyme is a thermophilic and halotolerant biocatalyst that is successfully employed in the synthesis of different purine ribonucleoside analogues, potential of AvNRT as an industrial biocatalyst for the synthesis of nucleoside analogues | Archaeoglobus veneficus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.5 | gene nrt, sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme AvNRT in Escherichia coli strain BL21(DE3) | Archaeoglobus veneficus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.5 | acetone | 50% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | acetonitril | 30% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | chloroform | 20% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | dimethylformamide | complete inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | DMSO | 80% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | ethanol | 35% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | ethyl acetate | 20% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | ethylene glycol | 80% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | glycerol | 35% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | Isopropanol | 20% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | methanol | 45% inhibition at 20% v/v | Archaeoglobus veneficus | |
2.4.2.5 | Propylene glycol | 78% inhibition at 20% v/v | Archaeoglobus veneficus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.5 | NaCl | the activity of AvNRT increases up to 2fold in 4 M NaCl aqueous solution | Archaeoglobus veneficus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.5 | D-ribosyl-base1 + base2 | Archaeoglobus veneficus | - |
D-ribosyl-base2 + base1 | - |
? | |
2.4.2.5 | D-ribosyl-base1 + base2 | Archaeoglobus veneficus DSM 11195 | - |
D-ribosyl-base2 + base1 | - |
? | |
2.4.2.5 | D-ribosyl-base1 + base2 | Archaeoglobus veneficus SNP6 | - |
D-ribosyl-base2 + base1 | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.4.2.5 | additional information | the enzyme activity is retained in the presence of several water-miscible organic solvents | Archaeoglobus veneficus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.5 | Archaeoglobus veneficus | F2KPY9 | a chemolithoautotrophic hyperthermophilic sulfitereducing archaeal coccus | - |
2.4.2.5 | Archaeoglobus veneficus DSM 11195 | F2KPY9 | a chemolithoautotrophic hyperthermophilic sulfitereducing archaeal coccus | - |
2.4.2.5 | Archaeoglobus veneficus SNP6 | F2KPY9 | a chemolithoautotrophic hyperthermophilic sulfitereducing archaeal coccus | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.5 | recombinant N-terminally His6-tagged enzyme AvNRT from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel filtration | Archaeoglobus veneficus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.5 | D-ribosyl-base1 + base2 | - |
Archaeoglobus veneficus | D-ribosyl-base2 + base1 | - |
? | |
2.4.2.5 | D-ribosyl-base1 + base2 | - |
Archaeoglobus veneficus DSM 11195 | D-ribosyl-base2 + base1 | - |
? | |
2.4.2.5 | D-ribosyl-base1 + base2 | - |
Archaeoglobus veneficus SNP6 | D-ribosyl-base2 + base1 | - |
? | |
2.4.2.5 | guanosine + 2,6-diaminopurine | - |
Archaeoglobus veneficus | guanine + 2,6-diaminopurine ribonucleoside | - |
? | |
2.4.2.5 | guanosine + 2,6-diaminopurine | - |
Archaeoglobus veneficus DSM 11195 | guanine + 2,6-diaminopurine ribonucleoside | - |
? | |
2.4.2.5 | guanosine + 2,6-diaminopurine | - |
Archaeoglobus veneficus SNP6 | guanine + 2,6-diaminopurine ribonucleoside | - |
? | |
2.4.2.5 | guanosine + 2-chloroadenine | - |
Archaeoglobus veneficus | guanine + 2-chloroadenosine | - |
? | |
2.4.2.5 | guanosine + 2-chloroadenine | - |
Archaeoglobus veneficus DSM 11195 | guanine + 2-chloroadenosine | - |
? | |
2.4.2.5 | guanosine + 2-chloroadenine | - |
Archaeoglobus veneficus SNP6 | guanine + 2-chloroadenosine | - |
? | |
2.4.2.5 | guanosine + 2-fluoroadenine | - |
Archaeoglobus veneficus | guanine + 2-fluoroadenosine | - |
? | |
2.4.2.5 | guanosine + 2-fluoroadenine | - |
Archaeoglobus veneficus DSM 11195 | guanine + 2-fluoroadenosine | - |
? | |
2.4.2.5 | guanosine + 2-fluoroadenine | - |
Archaeoglobus veneficus SNP6 | guanine + 2-fluoroadenosine | - |
? | |
2.4.2.5 | guanosine + 6-methoxyguanine | - |
Archaeoglobus veneficus | guanine + 6-methoxyguanosine | - |
? | |
2.4.2.5 | guanosine + adenine | - |
Archaeoglobus veneficus | guanine + adenosine | - |
? | |
2.4.2.5 | guanosine + hypoxanthine | - |
Archaeoglobus veneficus | guanine + inosine | - |
? | |
2.4.2.5 | additional information | substrate specificity of recombinant enzyme, overview. Binding structure of guanosine to the active site of AvNRT | Archaeoglobus veneficus | ? | - |
- |
|
2.4.2.5 | additional information | substrate specificity of recombinant enzyme, overview. Binding structure of guanosine to the active site of AvNRT | Archaeoglobus veneficus DSM 11195 | ? | - |
- |
|
2.4.2.5 | additional information | substrate specificity of recombinant enzyme, overview. Binding structure of guanosine to the active site of AvNRT | Archaeoglobus veneficus SNP6 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.5 | homotetramer | 4 * 18000, recombinant enzyme, SDS-PAGE, 4* 19245, recombinant enzyme, analytical ultracentrifugation | Archaeoglobus veneficus |
2.4.2.5 | More | three-dimensional modeling of AvNRT, overview | Archaeoglobus veneficus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.5 | AvNRT | - |
Archaeoglobus veneficus |
2.4.2.5 | N-ribosyltransferase | - |
Archaeoglobus veneficus |
2.4.2.5 | NRT | - |
Archaeoglobus veneficus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.5 | 80 | - |
- |
Archaeoglobus veneficus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.5 | 40 | 100 | activity range, profile overview | Archaeoglobus veneficus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.5 | 60 | 80 | the enzyme shows high stability at high temperatures | Archaeoglobus veneficus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.5 | 6 | - |
- |
Archaeoglobus veneficus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.5 | 4 | 8 | activity range, profile overview | Archaeoglobus veneficus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.2.5 | additional information | enzyme homology structure modeling and molecular dynamics simulations, overview. Nucleophilic attack on C1' by the carboxylate of the catalytic residue Glu85. Pro11 and Pro42 play a prominent role in making up the active site cavity where one of the Asp79 carboxylate oxygens and the carboxamide nitrogen of Asn113 recognize the O5' of the sugar moiety of the substrate while the backbone NH of Ala10 hydrogen bonds to O3'. The heteroaromatic ring of the nucleobase thus gets sandwiched between the phenyl rings of Phe12 and Phe13, on one side and the hydrophobic sidechains of Phe56 and Leu119 on the opposite side. The Met120 side chain sulfur appears close to the H1' atom, which suggests that it likely plays a role, together with the carboxylate of Asp62, in stabilization of the oxocarbenium reaction intermediate | Archaeoglobus veneficus |