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Literature summary extracted from

  • Galman, J.L.; Gahloth, D.; Parmeggiani, F.; Slabu, I.; Leys, D.; Turner, N.J.
    Characterization of a putrescine transaminase from Pseudomonas putida and its application to the synthesis of benzylamine derivatives (2018), Front. Bioeng. Biotechnol., 6, 205 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.6.1.82 synthesis putrescine transaminase Pp-SpuC application in the transamination of a comprehensive range of ketones/aldehydes for the production of a variety of benzylamine derivatives. Applications of enzyme Pp-SpuC as a biocatalyst with broad substrate tolerance Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.82 gene spuC, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.82 purified recombinant enzyme in apoform, sitting drop vapour diffusion method, best from 0.2 M MgCl2, 0.1 M Bis-Tris, pH 6.5, and 25% PEG 3350, at 20°C, X-ray diffraction structure determination analysis at 2.1 A resolution, molecular replacement method, structure modelling using the structure of aspartate aminotransferase from Pseudomonas sp. (PDB ID 5TI8) as the search model Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.82 putrescine + 2-oxoglutarate Pseudomonas putida
-
 1-pyrroline + L-glutamate + H2O
-
 ?
2.6.1.82 putrescine + 2-oxoglutarate Pseudomonas putida NBRC 14161
-
 1-pyrroline + L-glutamate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.82 Pseudomonas putida A0A4P8GNL5 i.e. Arthrobacter siderocapsulatus
-
2.6.1.82 Pseudomonas putida NBRC 14161 A0A4P8GNL5 i.e. Arthrobacter siderocapsulatus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.82 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.82 1H-indole-2-carbaldehyde + cadaverine
-
 Pseudomonas putida 1-(1H-indol-2-yl)methanamine + piperideine + H2O
-
 ?
2.6.1.82 1H-indole-2-carbaldehyde + cadaverine
-
 Pseudomonas putida NBRC 14161 1-(1H-indol-2-yl)methanamine + piperideine + H2O
-
 ?
2.6.1.82 1H-indole-2-carbaldehyde + isopropylamine
-
 Pseudomonas putida 1-(1H-indol-2-yl)methanamine + acetone + H2O
-
 ?
2.6.1.82 2,3-dimethoxybenzaldehyde + cadaverine
-
 Pseudomonas putida 3,5-dimethoxybenzylamine + piperideine + H2O
-
 ?
2.6.1.82 2,3-dimethoxybenzaldehyde + isopropylamine
-
 Pseudomonas putida 2,3-dimethoxybenzylamine + acetone + H2O
-
 ?
2.6.1.82 2,4-dimethoxybenzaldehyde + cadaverine
-
 Pseudomonas putida 2,4-dimethoxybenzylamine + piperideine + H2O
-
 ?
2.6.1.82 2,4-dimethoxybenzaldehyde + isopropylamine
-
 Pseudomonas putida 2,4-dimethoxybenzylamine + acetone + H2O
-
 ?
2.6.1.82 2-(trifluoromethyl)benzaldehyde + cadaverine
-
 Pseudomonas putida 2-(trifluoromethyl)benzylamine + piperideine + H2O
-
 ?
2.6.1.82 2-(trifluoromethyl)benzaldehyde + isopropylamine
-
 Pseudomonas putida 2-(trifluoromethyl)benzylamine + acetone + H2O
-
 ?
2.6.1.82 2-formylbenzoic acid + cadaverine
-
 Pseudomonas putida 2-(aminomethyl)benzoic acid + piperideine + H2O
-
 ?
2.6.1.82 2-formylbenzoic acid + isopropylamine
-
 Pseudomonas putida 2-(aminomethyl)benzoic acid + acetone + H2O
-
 ?
2.6.1.82 2-formylbenzonitrile + cadaverine
-
 Pseudomonas putida 2-(aminomethyl)benzonitrile + piperideine + H2O
-
 ?
2.6.1.82 2-formylbenzonitrile + isopropylamine
-
 Pseudomonas putida 2-(aminomethyl)benzonitrile + acetone + H2O
-
 ?
2.6.1.82 2-methoxybenzaldehyde + cadaverine
-
 Pseudomonas putida 2-methoxybenzylamine + piperideine + H2O
-
 ?
2.6.1.82 2-methoxybenzaldehyde + isopropylamine
-
 Pseudomonas putida 2-methoxybenzylamine + acetone + H2O
-
 ?
2.6.1.82 2-methoxypyridine-3-carbaldehyde + cadaverine
-
 Pseudomonas putida 1-(2-methoxypyridin-3-yl)methanamine + piperideine + H2O
-
 ?
2.6.1.82 2-methoxypyridine-3-carbaldehyde + isopropylamine
-
 Pseudomonas putida 1-(2-methoxypyridin-3-yl)methanamine + acetone + H2O
-
 ?
2.6.1.82 2H-1,3-benzodioxole-4-carbaldehyde + cadaverine
-
 Pseudomonas putida 1-(2H-1,3-benzodioxol-4-yl)methanamine + piperideine + H2O
-
 ?
2.6.1.82 2H-1,3-benzodioxole-4-carbaldehyde + isopropylamine
-
 Pseudomonas putida 1-(2H-1,3-benzodioxol-4-yl)methanamine + acetone + H2O
-
 ?
2.6.1.82 3,4-difluorobenzaldehyde + cadaverine
-
 Pseudomonas putida 3,4-difluorobenzylamine + piperideine + H2O
-
 ?
2.6.1.82 3,4-difluorobenzaldehyde + isopropylamine
-
 Pseudomonas putida 3,4-difluorobenzylamine + acetone + H2O
-
 ?
2.6.1.82 3,5-dibromobenzaldehyde + cadaverine
-
 Pseudomonas putida 3,5-dibromobenzylamine + piperideine + H2O
-
 ?
2.6.1.82 3,5-dibromobenzaldehyde + isopropylamine
-
 Pseudomonas putida 3,5-dibromobenzylamine + acetone + H2O
-
 ?
2.6.1.82 3,5-dimethoxybenzaldehyde + cadaverine
-
 Pseudomonas putida 3,5-dimethoxybenzylamine + piperideine + H2O
-
 ?
2.6.1.82 3,5-dimethoxybenzaldehyde + cadaverine
-
 Pseudomonas putida NBRC 14161 3,5-dimethoxybenzylamine + piperideine + H2O
-
 ?
2.6.1.82 3,5-dimethoxybenzaldehyde + isopropylamine
-
 Pseudomonas putida 3,5-dimethoxybenzylamine + acetone + H2O
-
 ?
2.6.1.82 3-acetylbenzoic acid + cadaverine
-
 Pseudomonas putida 3-(1-aminoethyl)benzoic acid + piperideine + H2O
-
 ?
2.6.1.82 3-acetylbenzoic acid + isopropylamine
-
 Pseudomonas putida 3-(1-aminoethyl)benzoic acid + acetone + H2O
-
 ?
2.6.1.82 3-chlorobenzaldehyde + cadaverine
-
 Pseudomonas putida 3-chlorobenzylamine + piperideine + H2O
-
 ?
2.6.1.82 3-chlorobenzaldehyde + isopropylamine
-
 Pseudomonas putida 3-chlorobenzylamine + acetone + H2O
-
 ?
2.6.1.82 3-fluorobenzaldehyde + cadaverine
-
 Pseudomonas putida 3-fluorobenzylamine + piperideine + H2O
-
 ?
2.6.1.82 3-fluorobenzaldehyde + isopropylamine
-
 Pseudomonas putida 3-fluorobenzylamine + acetone + H2O
-
 ?
2.6.1.82 3-formylbenzonitrile + cadaverine
-
 Pseudomonas putida 3-(aminomethyl)benzonitrile + piperideine + H2O
-
 ?
2.6.1.82 3-formylbenzonitrile + isopropylamine
-
 Pseudomonas putida 3-(aminomethyl)benzonitrile + acetone + H2O
-
 ?
2.6.1.82 3-methoxybenzaldehyde + cadaverine
-
 Pseudomonas putida 3-methoxybenzylamine + piperideine + H2O
-
 ?
2.6.1.82 3-methoxybenzaldehyde + isopropylamine
-
 Pseudomonas putida 3-methoxybenzylamine + acetone + H2O
-
 ?
2.6.1.82 3-nitrobenzaldehyde + cadaverine
-
 Pseudomonas putida 3-nitrobenzylamine + piperideine + H2O
-
 ?
2.6.1.82 3-nitrobenzaldehyde + isopropylamine
-
 Pseudomonas putida 3-nitrobenzylamine + acetone + H2O
-
 ?
2.6.1.82 4-acetylbenzoic acid + cadaverine
-
 Pseudomonas putida 4-(1-aminoethyl)benzoic acid + piperideine + H2O
-
 ?
2.6.1.82 4-acetylbenzoic acid + isopropylamine
-
 Pseudomonas putida 4-(1-aminoethyl)benzoic acid + acetone + H2O
-
 ?
2.6.1.82 4-chlorobenzaldehyde + cadaverine
-
 Pseudomonas putida 4-chlorobenzylamine + piperideine + H2O
-
 ?
2.6.1.82 4-chlorobenzaldehyde + isopropylamine
-
 Pseudomonas putida 4-chlorobenzylamine + acetone + H2O
-
 ?
2.6.1.82 4-fluorobenzaldehyde + cadaverine
-
 Pseudomonas putida 4-fluorobenzylamine + piperideine + H2O
-
 ?
2.6.1.82 4-fluorobenzaldehyde + isopropylamine
-
 Pseudomonas putida 4-fluorobenzylamine + acetone + H2O
-
 ?
2.6.1.82 4-formylbenzoic acid + cadaverine
-
 Pseudomonas putida 4-(aminomethyl)benzoic acid + piperideine + H2O
-
 ?
2.6.1.82 4-formylbenzoic acid + isopropylamine
-
 Pseudomonas putida 4-(aminomethyl)benzoic acid + acetone + H2O
-
 ?
2.6.1.82 4-formylbenzonitrile + cadaverine
-
 Pseudomonas putida 4-(aminomethyl)benzonitrile + piperideine + H2O
-
 ?
2.6.1.82 4-formylbenzonitrile + isopropylamine
-
 Pseudomonas putida 4-(aminomethyl)benzonitrile + acetone + H2O
-
 ?
2.6.1.82 4-nitrobenzaldehyde + cadaverine
-
 Pseudomonas putida 4-nitrobenzylamine + piperideine + H2O
-
 ?
2.6.1.82 4-nitrobenzaldehyde + isopropylamine
-
 Pseudomonas putida 4-nitrobenzylamine + acetone + H2O
-
 ?
2.6.1.82 5-chlorothiophene-2-carbaldehyde + cadaverine
-
 Pseudomonas putida 1-(5-chlorothiophen-2-yl)methanamine + piperideine + H2O
-
 ?
2.6.1.82 5-chlorothiophene-2-carbaldehyde + isopropylamine
-
 Pseudomonas putida 1-(5-chlorothiophen-2-yl)methanamine + acetone + H2O
-
 ?
2.6.1.82 5-formylthiophene-2-carboxylic acid + cadaverine
-
 Pseudomonas putida 5-(aminomethyl)thiophene-2-carboxylic acid + piperideine + H2O
-
 ?
2.6.1.82 5-formylthiophene-2-carboxylic acid + isopropylamine
-
 Pseudomonas putida 5-(aminomethyl)thiophene-2-carboxylic acid + acetone + H2O
-
 ?
2.6.1.82 5-methylfuran-2-carbaldehyde + cadaverine
-
 Pseudomonas putida 1-(5-methylfuran-2-yl)methanamine + piperideine + H2O
-
 ?
2.6.1.82 5-methylfuran-2-carbaldehyde + isopropylamine
-
 Pseudomonas putida 1-(5-methylfuran-2-yl)methanamine + acetone + H2O
-
 ?
2.6.1.82 6-acetylpyridine-2-carboxylic acid + cadaverine
-
 Pseudomonas putida 6-(1-aminoethyl)pyridine-2-carboxylic acid + piperideine + H2O
-
 ?
2.6.1.82 6-acetylpyridine-2-carboxylic acid + isopropylamine
-
 Pseudomonas putida 6-(1-aminoethyl)pyridine-2-carboxylic acid + acetone + H2O
-
 ?
2.6.1.82 6-methoxypyridine-3-carbaldehyde + cadaverine
-
 Pseudomonas putida 1-(6-methoxypyridin-3-yl)methanamine + piperideine + H2O
-
 ?
2.6.1.82 6-methoxypyridine-3-carbaldehyde + isopropylamine
-
 Pseudomonas putida 1-(6-methoxypyridin-3-yl)methanamine + acetone + H2O
-
 ?
2.6.1.82 cadaverine + 2-oxoglutarate
-
 Pseudomonas putida piperideine + L-glutamate + H2O
-
 ?
2.6.1.82 isopropylamine + 2-oxoglutarate
-
 Pseudomonas putida acetone + L-glutamate + H2O
-
 ?
2.6.1.82 additional information the enzyme shows dual substrate recognition for biogenic diamines (i.e. cadaverine) and readily available monoamines (i.e. isopropylamine) for the synthesis of benzylamine derivatives with excellent product conversions and extremely broad substrate tolerance. Biocatalytic synthesis of a range of substituted benzylamines mediated by Pp-SpuC in a coupled assay approach with cadaverine or isopropylamine as co-substrate at 50 mM Tris-HCl, pH 9.0, 30°C, 1 mM PLP, and 1% v/v DMSO, overview Pseudomonas putida ?
-
-
2.6.1.82 additional information the enzyme shows dual substrate recognition for biogenic diamines (i.e. cadaverine) and readily available monoamines (i.e. isopropylamine) for the synthesis of benzylamine derivatives with excellent product conversions and extremely broad substrate tolerance. Biocatalytic synthesis of a range of substituted benzylamines mediated by Pp-SpuC in a coupled assay approach with cadaverine or isopropylamine as co-substrate at 50 mM Tris-HCl, pH 9.0, 30°C, 1 mM PLP, and 1% v/v DMSO, overview Pseudomonas putida NBRC 14161 ?
-
-
2.6.1.82 putrescine + 2-oxoglutarate
-
 Pseudomonas putida 1-pyrroline + L-glutamate + H2O
-
 ?
2.6.1.82 putrescine + 2-oxoglutarate overall reaction Pseudomonas putida 1-pyrroline + L-glutamate + H2O
-
 ?
2.6.1.82 putrescine + 2-oxoglutarate
-
 Pseudomonas putida NBRC 14161 1-pyrroline + L-glutamate + H2O
-
 ?
2.6.1.82 putrescine + 2-oxoglutarate overall reaction Pseudomonas putida NBRC 14161 1-pyrroline + L-glutamate + H2O
-
 ?
2.6.1.82 quinoline-3-carbaldehyde + cadaverine
-
 Pseudomonas putida 1-(quinolin-3-yl)methanamine + piperideine + H2O
-
 ?
2.6.1.82 quinoline-3-carbaldehyde + isopropylamine
-
 Pseudomonas putida 1-(quinolin-3-yl)methanamine + acetone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.82 dimer the enzyme forms a dimer that adopts a class I transaminase fold comparable to other class III transaminases Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
2.6.1.82 Pp-SpuC
-
 Pseudomonas putida
2.6.1.82 putrescine transaminase
-
 Pseudomonas putida
2.6.1.82 SpuC
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.82 60
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.82 20 90 activity range, profile overview. The activity of the enzyme significantly improves as the temperature increased to its suboptimum activity at 50°C, and reaches 100% optimum relative activity at 60°C. At higher temperature, it shows gradual denaturation but maintaining significant residual activity (77%) up to 70°C. Over 50% of maximal activity at 45-75°C Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.82 50
-
 purified recombinant enzyme in Tris-HCl, pH 8.0, half-life is 7.7 h Pseudomonas putida
2.6.1.82 60
-
 purified recombinant enzyme in Tris-HCl, pH 8.0, half-life is 37 min Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.82 8
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.82 6.2 10.2 activity range, profile overview Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.82 pyridoxal 5'-phosphate PLP Pseudomonas putida

General Information

EC Number General Information Comment Organism
2.6.1.82 evolution the enzyme forms a dimer that adopts a class I transaminase fold comparable to other class III transaminases Pseudomonas putida
2.6.1.82 metabolism promiscuous role of Pseudomonas putida Pp-SpuC in metabolism Pseudomonas putida
2.6.1.82 additional information schematic representation of the amino acid residues forming the small and large binding pockets of Pp-SpuC around the external aldimine intermediate (PLP-substrate adduct). The model is based on the multiple sequence alignment analysis and on the active site structure of Vf-omega transaminase (PDB ID 4E3Q) Pseudomonas putida