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Literature summary extracted from

  • Sirikharin, R.; Soederhaell, I.; Soederhaell, K.
    Characterization of a cold-active transglutaminase from a crayfish, Pacifastacus leniusculus (2018), Fish Shellfish Immunol., 80, 546-549 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 EDTA complete inhibition at 10 mM Cavia porcellus
2.3.2.13 EDTA complete inhibition at 10 mM Pacifastacus leniusculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 additional information
-
 additional information kinetics, comparison to the kinetics of commercial enzyme from guinea pig liver Pacifastacus leniusculus
2.3.2.13 additional information
-
 additional information kinetics, comparison to the kinetics of commercial enzyme from guinea pig liver Cavia porcellus
2.3.2.13 0.00288
-
 pentylamine pH 8.5, 4°C Pacifastacus leniusculus
2.3.2.13 0.0029
-
 pentylamine pH 8.5, 22°C Pacifastacus leniusculus
2.3.2.13 0.0203
-
 pentylamine pH 8.5, 22°C Cavia porcellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ dependent on Pacifastacus leniusculus
2.3.2.13 Ca2+ dependent on Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.13 protein glutamine + alkylamine Pacifastacus leniusculus
-
 protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine Cavia porcellus
-
 protein N5-alkylglutamine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Cavia porcellus A0A286XL02
-
-
2.3.2.13 Pacifastacus leniusculus Q964D3 from from Lake Erken, Schweden
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 commercial preparation
-
 Cavia porcellus
-
2.3.2.13 hematopoietic cell
-
 Pacifastacus leniusculus
-
2.3.2.13 liver
-
 Cavia porcellus
-
2.3.2.13 additional information signal crayfish is a 60 long-lived species and its natural habitat, fresh waters usually have a wide range of temperatures from below 7°C during winter and up to more than 20°C in summer Pacifastacus leniusculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 casein glutamine + pentylamine the enzymatic activity of TGase is determined by measuring the fluorescence of MDC cross-linking to N,N'-dimethyl casein upon exposure Pacifastacus leniusculus casein N5-pentylglutamine + NH3
-
 ?
2.3.2.13 casein glutamine + pentylamine the enzymatic activity of TGase is determined by measuring the fluorescence of MDC cross-linking to N,N'-dimethyl casein upon exposure Cavia porcellus casein N5-pentylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine
-
 Pacifastacus leniusculus protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine
-
 Cavia porcellus protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine transglutaminases (TGases) catalyze a Ca2+-dependent acyl transfer reaction between the epsilon-amino group of lysine (acyl acceptors) and gamma-carboxamide groups of glutamine residues (acyl donors) and forms cross-links by catalyzing the isopeptide bond formation between Lys and Gln residues to form epsilon-(gamma-glutamyl)lysine bonds between appropriate substrates Pacifastacus leniusculus protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 protein glutamine + alkylamine transglutaminases (TGases) catalyze a Ca2+-dependent acyl transfer reaction between the epsilon-amino group of lysine (acyl acceptors) and gamma-carboxamide groups of glutamine residues (acyl donors) and forms cross-links by catalyzing the isopeptide bond formation between Lys and Gln residues to form epsilon-(gamma-glutamyl)lysine bonds between appropriate substrates Cavia porcellus protein N5-alkylglutamine + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 cold-active transglutaminase
-
 Pacifastacus leniusculus
2.3.2.13 TGase
-
 Pacifastacus leniusculus
2.3.2.13 TGase 1
-
 Cavia porcellus
2.3.2.13 transglutaminase
-
 Pacifastacus leniusculus
2.3.2.13 transglutaminase 1
-
 Cavia porcellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 22
-
 assay at room temperature Pacifastacus leniusculus
2.3.2.13 22
-
 assay at room temperature Cavia porcellus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.2.13 4 37 the guinea pig liver enzyme shows very low activity at 4°C and high activity at 37°C Cavia porcellus
2.3.2.13 4 22 at 4°C, crayfish TGase shows high activity due to a decreasing Km value for pentylamine as a substrate, the kcat value is not affected at 22°C Pacifastacus leniusculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.13 0.0065
-
 pentylamine pH 8.5, 4°C Pacifastacus leniusculus
2.3.2.13 0.0096
-
 pentylamine pH 8.5, 22°C Pacifastacus leniusculus
2.3.2.13 0.064
-
 pentylamine pH 8.5, 22°C Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 8.5
-
-
 Pacifastacus leniusculus
2.3.2.13 8.5
-
-
 Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.13 2 10 activity range, low activity at pH 2.0, high activity at pH 7.4-8.5, maximal activity at pH 8.5, 60% of maximal activity at pH 10.0, profile overview Pacifastacus leniusculus
2.3.2.13 2 10 activity range, low activity at pH 2.0, high activity at pH 7.4-8.5, maximal activity at pH 8.5, 60% of maximal activity at pH 10.0, profile overview Cavia porcellus

General Information

EC Number General Information Comment Organism
2.3.2.13 evolution crayfish TGase is adapted to have significant activity at low temperatures since crayfish are living in quite cold waters Pacifastacus leniusculus
2.3.2.13 additional information comparison of the crayfish enzyme to the commercial enzyme from guinea pig liver, which is highly active at 37°C Pacifastacus leniusculus
2.3.2.13 additional information comparison of the crayfish enzyme, which is highly active at 4°C, to the commercial enzyme from guinea pig liver Cavia porcellus
2.3.2.13 physiological function transglutaminases (TGases) catalyze a Ca2+-dependent acyl transfer reaction between the epsilon-amino group of lysine (acyl acceptors) and gamma-carboxamide groups of glutamine residues (acyl donors) and forms cross-links by catalyzing the isopeptide bond formation between Lys and Gln residues to form epsilon-(gamma-glutamyl) lysine bonds between appropriate substrates. This reaction is essential for physiological cell functions, such as in blood coagulation, during cell differentiation and survival, skin formation, and signal transduction Pacifastacus leniusculus
2.3.2.13 physiological function transglutaminases (TGases) catalyze a Ca2+-dependent acyl transfer reaction between the epsilon-amino group of lysine (acyl acceptors) and gamma-carboxamide groups of glutamine residues (acyl donors) and forms cross-links by catalyzing the isopeptide bond formation between Lys and Gln residues to form epsilon-(gamma-glutamyl) lysine bonds between appropriate substrates. This reaction is essential for physiological cell functions, such as in blood coagulation, during cell differentiation and survival, skin formation, and signal transduction Cavia porcellus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.13 2.26
-
 pentylamine pH 8.5, 4°C Pacifastacus leniusculus
2.3.2.13 3.15
-
 pentylamine pH 8.5, 22°C Cavia porcellus
2.3.2.13 3.31
-
 pentylamine pH 8.5, 22°C Pacifastacus leniusculus