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Literature summary extracted from

  • Rubat, S.; Varas, I.; Sepulveda, R.; Almonacid, D.; Gonzalez-Nilo, F.; Agosin, E.
    Increasing the intracellular isoprenoid pool in Saccharomyces cerevisiae by structural fine-tuning of a bifunctional farnesyl diphosphate synthase (2017), FEMS Yeast Res., 17, fox032 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.1 A99H the strain carrying the variant shows significant differences on geranyl diphosphate concentrations and specific growth rate Saccharomyces cerevisiae
2.5.1.1 A99K the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae
2.5.1.1 A99Q the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae
2.5.1.1 A99R the strain carrying the variant shows significant differences on geranyl diphosphate concentrations and specific growth rate Saccharomyces cerevisiae
2.5.1.1 A99T the strain carrying the variant produces unquantifiable amounts of farnesyl diphosphate and no effect on geranyl diphosphate production is observed Saccharomyces cerevisiae
2.5.1.1 A99Y the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae
2.5.1.10 A99G mutant enzyme shows a significant increase in the total concentration of isoprene: 2.4times compared to the wild-type strain. No detection of geranyl diphosphate Saccharomyces cerevisiae
2.5.1.10 A99H the strain carrying the variant shows significant differences on geranyl diphosphate concentrations and specific growth rate Saccharomyces cerevisiae
2.5.1.10 A99H variant shows significant difference on geranyl diphosphate concentrations and specific growth rate Saccharomyces cerevisiae
2.5.1.10 A99K no detection of geranyl diphosphate Saccharomyces cerevisiae
2.5.1.10 A99K the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae
2.5.1.10 A99P mutant enzyme shows a significant increase in the total concentration of isoprene: 6.0times compared to the wild-type strain. The mutant enzyme shows a significant accumulation of isoprene phosphate particularly of isopentenyl diphosphate and/or dimethylallyl diphosphate, which are not detected in the wild-type strain. 1.5fold increase in geranyl diphosphate concentration compared to wild-type enzyme Saccharomyces cerevisiae
2.5.1.10 A99Q mutant enzyme shows a significant increase in the total concentration of isoprene: 8.0times compared to the wild-type strain. The mutant enzyme shows a significant accumulation of isoprene phosphate particularly of isopentenyl diphosphate and/or dimethylallyl diphosphate, which are not detected in the wild-type strain. No detection of geranyl diphosphate Saccharomyces cerevisiae
2.5.1.10 A99Q the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae
2.5.1.10 A99R the strain carrying the variant shows significant differences on geranyl diphosphate concentrations and specific growth rate Saccharomyces cerevisiae
2.5.1.10 A99R variant shows significant difference on geranyl diphosphate concentrations and specific growth rate Saccharomyces cerevisiae
2.5.1.10 A99T mutant enzyme behaves similarly to the wild-type strain with respect to the geranyl diphosphate content Saccharomyces cerevisiae
2.5.1.10 A99T the mutant produces unquantifiable amounts of farnesyl diphosphate and no effect on geranyl diphosphate production is observed Saccharomyces cerevisiae
2.5.1.10 A99T the strain carrying the variant produces unquantifiable amounts of farnesyl diphosphate and no effect on geranyl diphosphate production is observed Saccharomyces cerevisiae
2.5.1.10 A99V mutant enzyme behaves similarly to the wild-type strain with respect to the geranyl diphosphate content Saccharomyces cerevisiae
2.5.1.10 A99W mutant enzyme shows a significant increase in the total concentration of isoprene: 2.1times compared to the wild-type strain. 3fold increase in geranyl diphosphate concentration compared to wild-type enzyme Saccharomyces cerevisiae
2.5.1.10 A99Y mutant enzyme shows a significant accumulation of isoprene phosphate particularly of isopentenyl diphosphate and/or dimethylallyl diphosphate, which are not detected in the wild-type strain Saccharomyces cerevisiae
2.5.1.10 A99Y mutant enzyme shows a significant increase in the total concentration of isoprene: 3.1times compared to the wild-type strain. The mutant enzyme shows a significant accumulation of isoprene phosphate particularly of isopentenyl diphosphate and/or dimethylallyl diphosphate, which are not detected in the wild-type strain. No detection of geranyl diphosphate Saccharomyces cerevisiae
2.5.1.10 A99Y the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.1 dimethylallyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate diphosphate + geranyl diphosphate
-
 ?
2.5.1.1 geranyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae reaction of EC 2.5.1.10. The enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae BY4741 reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae BY4741 the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae BY4741 the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Saccharomyces cerevisiae BY4741 the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.1 Saccharomyces cerevisiae
-
-
-
2.5.1.10 Saccharomyces cerevisiae P08524 S288c-derivative laboratory strain
-
2.5.1.10 Saccharomyces cerevisiae BY4741 P08524 S288c-derivative laboratory strain
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.1 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + geranyl diphosphate
-
 ?
2.5.1.1 geranyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.10. The enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae BY4741 diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae BY4741 diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate Saccharomyces cerevisiae BY4741 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate Saccharomyces cerevisiae BY4741 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.1 ERG20
-
 Saccharomyces cerevisiae
2.5.1.10 ERG20
-
 Saccharomyces cerevisiae
2.5.1.10 FPPS
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.5.1.1 metabolism farnesyl diphosphate synthase is a key enzyme responsible for the supply of isoprenoid precursors for several essential metabolites, including sterols, dolichols and ubiquinone Saccharomyces cerevisiae
2.5.1.10 metabolism farnesyl diphosphate synthase is a key enzyme responsible for the supply of isoprenoid precursors for several essential metabolites, including sterols, dolichols and ubiquinone Saccharomyces cerevisiae
2.5.1.10 physiological function key enzyme responsible for the supply of isoprenoid precursors for several essential metabolites, including sterols, dolichols and ubiquinone Saccharomyces cerevisiae