Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Taestensen, J.B.; Johnsen, U.; Reinhardt, A.; Ortjohann, M.; Schoenheit, P.
    D-galactose catabolism in archaea operation of the DeLey-Doudoroff pathway in Haloferax volcanii (2020), FEMS Microbiol. Lett., 367, fnaa029 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.376 overexpression in Haloferax volcanii H1209 Haloferax volcanii
2.7.1.58 gene kdgK1, recombinant overexpression in Haloferax volcanii strain H1209 Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.376 0.32
-
 NADP+ pH 8.0, temperature not specified in the publication Haloferax volcanii
1.1.1.376 2 3 D-galactose pH 8.0, temperature not specified in the publication Haloferax volcanii
1.1.1.376 35
-
 L-arabinose pH 8.0, temperature not specified in the publication Haloferax volcanii
2.7.1.58 0.2
-
 2-dehydro-3-deoxy-D-galactonate pH and temperature not specified in the publication Haloferax volcanii
2.7.1.58 0.3
-
 ATP pH and temperature not specified in the publication Haloferax volcanii
2.7.1.58 8.8
-
 2-dehydro-3-deoxy-D-gluconate pH and temperature not specified in the publication Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.376 KCl maximal activities are obtained at KCl concentrations higher than 1 M Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.376 76000
-
-
 Haloferax volcanii
2.7.1.58 194000
-
-
 Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.376 D-galactose + NADP+ Haloferax volcanii the enzyme has an essential function during growth on D-galactose D-galactono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.376 D-galactose + NADP+ Haloferax volcanii ATCC 29605 the enzyme has an essential function during growth on D-galactose D-galactono-1,4-lactone + NADPH + H+
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii NCIMB 2012
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii JCM 8879
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii H26
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii DS2
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii DSM 3757
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii ATCC 29605
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii NBRC 14742
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate Haloferax volcanii VKM B-1768
-
 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.376 Haloferax volcanii D4GR07
-
-
1.1.1.376 Haloferax volcanii ATCC 29605 D4GR07
-
-
2.7.1.58 Haloferax volcanii D4GR05
-
-
2.7.1.58 Haloferax volcanii ATCC 29605 D4GR05
-
-
2.7.1.58 Haloferax volcanii DS2 D4GR05
-
-
2.7.1.58 Haloferax volcanii DSM 3757 D4GR05
-
-
2.7.1.58 Haloferax volcanii H26 D4GR05
-
-
2.7.1.58 Haloferax volcanii JCM 8879 D4GR05
-
-
2.7.1.58 Haloferax volcanii NBRC 14742 D4GR05
-
-
2.7.1.58 Haloferax volcanii NCIMB 2012 D4GR05
-
-
2.7.1.58 Haloferax volcanii VKM B-1768 D4GR05
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.376
-
 Haloferax volcanii
2.7.1.58 recombinant enzyme from Haloferax volcanii strain H1209 Haloferax volcanii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.58 culture condition:D-galactose-grown cell
-
 Haloferax volcanii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.376 323
-
 substrate: D-galactose, pH 8.0, temperature not specified in the publication Haloferax volcanii
1.1.1.376 376
-
 substrate: L-arabinose, pH 8.0, temperature not specified in the publication Haloferax volcanii
2.7.1.58 0.148
-
 purified recombinant enzyme, pH 8.0, 42°C Haloferax volcanii
2.7.1.58 148
-
 substrate: 2-dehydro-3-deoxy-D-galactonate, pH and temperature not specified in the publication Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.376 D-galactose + NAD+ + H2O the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor Haloferax volcanii D-galactonate + NADH + H+
-
 ?
1.1.1.376 D-galactose + NAD+ + H2O the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor Haloferax volcanii ATCC 29605 D-galactonate + NADH + H+
-
 ?
1.1.1.376 D-galactose + NADP+ the enzyme has an essential function during growth on D-galactose Haloferax volcanii D-galactono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.376 D-galactose + NADP+ the enzyme has an essential function during growth on D-galactose Haloferax volcanii ATCC 29605 D-galactono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.376 D-galactose + NADP+ + H2O the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor Haloferax volcanii D-galactonate + NADPH + H+
-
 ?
1.1.1.376 D-galactose + NADP+ + H2O the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor Haloferax volcanii ATCC 29605 D-galactonate + NADPH + H+
-
 ?
1.1.1.376 L-arabinose + NADP+ + H2O
-
 Haloferax volcanii L-arabinonate + NADPH + H+
-
 ?
1.1.1.376 L-arabinose + NADP+ + H2O
-
 Haloferax volcanii ATCC 29605 L-arabinonate + NADPH + H+
-
 ?
1.1.1.376 additional information low activity was observed with D-xylose (5%), D-glucose, D-fructose and D-mannose are not oxidized at significant rates Haloferax volcanii ?
-
-
1.1.1.376 additional information low activity was observed with D-xylose (5%), D-glucose, D-fructose and D-mannose are not oxidized at significant rates Haloferax volcanii ATCC 29605 ?
-
-
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii NCIMB 2012 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii JCM 8879 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii H26 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii DS2 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii DSM 3757 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii ATCC 29605 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii NBRC 14742 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Haloferax volcanii VKM B-1768 ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii NCIMB 2012 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii JCM 8879 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii H26 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii DS2 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii DSM 3757 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii ATCC 29605 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii NBRC 14742 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?
2.7.1.58 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Haloferax volcanii VKM B-1768 ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.376 homodimer 2 * 39000, calculated from sequence Haloferax volcanii
2.7.1.58 hexamer 6 * 34000, calculated from sequence Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.376 HVO_A0330
-
 Haloferax volcanii
2.7.1.58 2-keto-3-deoxygalactonate kinase
-
 Haloferax volcanii
2.7.1.58 HVO A0328
-
 Haloferax volcanii
2.7.1.58 HVO_A0328
-
 Haloferax volcanii
2.7.1.58 KDGal kinase
-
 Haloferax volcanii
2.7.1.58 KDGK2 UniProt Haloferax volcanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.58 42
-
 assay at Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.376 8
-
-
 Haloferax volcanii
2.7.1.58 8
-
 assay at Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.376 NAD+ the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor Haloferax volcanii
1.1.1.376 NADP+ the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor Haloferax volcanii

Expression

EC Number Organism Comment Expression
2.7.1.58 Haloferax volcanii transcriptional regulator GacR is an activator of genes of the DeLey-Doudoroff pathway including the gene that encodes 2-keto-3-deoxygalactonate kinase up

General Information

EC Number General Information Comment Organism
1.1.1.376 malfunction the deletion mutant does not grow on D-galactose Haloferax volcanii
1.1.1.376 metabolism the enzyme is part of the De Ley-Doudoroff pathway Haloferax volcanii
2.7.1.58 malfunction growth studies with knock-out mutants indicate the functional involvement of galactose dehydrogenase, galactonate dehydratase, and KDPGal aldolase in D-galactose degradation Haloferax volcanii
2.7.1.58 malfunction the deletion mutant does not grow on D-galactose Haloferax volcanii
2.7.1.58 metabolism a cluster of genes encoding putative enzymes of the DeLey-Doudoroff pathway for D-galactose degradation including galactose dehydrogenase, galactonate dehydratase, 2-keto-3-deoxygalactonate kinase and 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) aldolase is involved in D-galactose degradation. The transcriptional regulator GacR is an activator of genes of the DeLey-Doudoroff pathway Haloferax volcanii
2.7.1.58 metabolism the enzyme is part of the De Ley-Doudoroff pathway Haloferax volcanii
2.7.1.58 physiological function the enzyme is required for D-galactose degradation via the DeLey-Doudoroff pathway in the domain of archaea Haloferax volcanii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.376 7
-
 L-arabinose pH 8.0, temperature not specified in the publication Haloferax volcanii
1.1.1.376 9.1
-
 D-galactose pH 8.0, temperature not specified in the publication Haloferax volcanii
2.7.1.58 8
-
 2-dehydro-3-deoxy-D-gluconate pH and temperature not specified in the publication Haloferax volcanii
2.7.1.58 432
-
 2-dehydro-3-deoxy-D-galactonate pH and temperature not specified in the publication Haloferax volcanii