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Literature summary extracted from

  • Sasidharan, S.; Saudagar, P.
    Biochemical and structural characterization of tyrosine aminotransferase suggests broad substrate specificity and a two-state folding mechanism in Leishmania donovani (2019), FEBS Open Bio, 9, 1769-1783 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.5 expressed in Escherichia coli BL21 cells Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.5 Urea the enzyme shows a gradual decrease in the activity till 2.5 M urea, after which the complete loss of activity is measured Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.7809
-
 L-tyrosine with alpha-ketomethiobutyrate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
2.6.1.5 0.8103
-
 alpha-ketomethiobutyrate with L-tyrosine as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
2.6.1.5 3.5
-
 L-tyrosine with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
2.6.1.5 3.572
-
 L-phenylalanine with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
2.6.1.5 5.055
-
 L-tryptophan with pyruvate as cosubstrate, at pH 8.0 and 37°C Leishmania donovani
2.6.1.5 42.67
-
 pyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 37°C Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.5 98600
-
 gel filtration Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Leishmania donovani A0A5B8TZA8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.5 Ni-NTA column chromatography Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-phenylalanine + pyruvate
-
 Leishmania donovani phenylpyruvate + L-alanine
-
 r
2.6.1.5 L-tryptophan + pyruvate
-
 Leishmania donovani 3-(1H-indol-3-yl)-2-oxopropanoate + L-alanine
-
 r
2.6.1.5 L-tyrosine + alpha-ketomethiobutyrate
-
 Leishmania donovani ?
-
 r
2.6.1.5 L-tyrosine + pyruvate
-
 Leishmania donovani 4-hydroxyphenylpyruvate + L-alanine
-
 r
2.6.1.5 additional information the enzyme exhibits no activity with L-tyrosine and 2-oxoglutarate Leishmania donovani ?
-
-

Subunits

EC Number Subunits Comment Organism
2.6.1.5 homodimer x * 49300, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
2.6.1.5 L-tyrosine: 2-oxoglutarate aminotransferase
-
 Leishmania donovani
2.6.1.5 TAT
-
 Leishmania donovani
2.6.1.5 TATase
-
 Leishmania donovani
2.6.1.5 tyrosine aminotransferase
-
 Leishmania donovani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.5 50
-
-
 Leishmania donovani

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.5 20 80 more than 90% activity between 20 and 70°C, about 70% activity at 80°C, about 20% activity at 90°C, no activity at 100°C Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 8
-
-
 Leishmania donovani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.5 6 9 more than 70% activity between pH 6.0 and 9.0 Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.5 pyridoxal 5'-phosphate Km value of 0.0235 mM Leishmania donovani