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Literature summary extracted from

  • Perveen, S.; Rashid, N.; Tang, X.F.; Imanaka, T.; Papageorgiou, A.C.
    Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Thermococcus kodakarensis shows maximum activity with zinc and forms a unique dimeric structure (2017), FEBS Open Bio, 7, 1217-1230 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.18 expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.18 free enzyme and in the presence of Zn2+, hanging drop vapor diffusion method, using 0.2 M sodium acetate (pH 7.5), 20% (w/v) PEG 3350 Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.18 anthranilate substrate inhibition. Concentrations above 0.004 mM result in reduced enzymatic activity Thermococcus kodakarensis
2.4.2.18 Co2+
-
 Thermococcus kodakarensis
2.4.2.18 Mg2+ concentrations higher than 0.2 mM Mg2+ inhibit the enzyme activity (about 85% residual activity at 0.25 mM, about 70% residual activity at 0.5 mM, about 60% residual activity at 1.0 mM) Thermococcus kodakarensis
2.4.2.18 Mn2+
-
 Thermococcus kodakarensis
2.4.2.18 Zn2+ concentrations higher than 0.1 mM Zn2+ inhibit the enzyme activity (about 80% residual activity at 0.3 mM, about 65% residual activity at 0.5 mM, about 20% residual activity at 1.0 mM) Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.18 0.0022
-
 anthranilate at pH 8.5 and 55°C Thermococcus kodakarensis
2.4.2.18 0.25
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 55°C Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.18 Ca2+ activates Thermococcus kodakarensis
2.4.2.18 Cu2+ slightly activates Thermococcus kodakarensis
2.4.2.18 Mg2+ activates at 0.2 mM Thermococcus kodakarensis
2.4.2.18 additional information not influenced by Ni2+ Thermococcus kodakarensis
2.4.2.18 Zn2+ the enzyme shows highest activity with 0.1 mM Zn2+. The enzyme contains a total of 6 Zn2+ ions in the dimer Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.18 70000
-
 gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Thermococcus kodakarensis
-
 N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.18 Thermococcus kodakarensis Q9YGB4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.18 anion exchange QFF column chromatography, Resource Q column chromatography and Superdex 75 gel filtration Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.18 1580
-
 at pH 8.5 and 55°C Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermococcus kodakarensis N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.18 homodimer 2 * 36000, SDS-PAGE Thermococcus kodakarensis
2.4.2.18 homodimer 2 * 34347, calculated from amino acid sequence Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.18 TrpD
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.18 55
-
-
 Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.18 8.5 9
-
 Thermococcus kodakarensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.2.18 Thermococcus kodakarensis calculated from amino acid sequence
-
 4.9