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Literature summary extracted from

  • Payer, S.; Schrittwieser, J.; Kroutil, W.
    Vicinal diamines as smart cosubstrates in the transaminase-catalyzed asymmetric amination of ketones (2017), Eur. J. Org. Chem., 2017, 2553-2559 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.B16 isooctane
-
 Chromobacterium violaceum
2.6.1.B16 methyl tert-butyl ether
-
 Chromobacterium violaceum
2.6.1.B16 Toluene
-
 Chromobacterium violaceum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.B16 recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli BL21(DE3) Chromobacterium violaceum

General Stability

EC Number General Stability Organism
2.6.1.B16 the monomer-dimer equilibrium has an effect on enzyme storage stability. After 3-4 days of incubation, the enzyme activity starts to decrease at the same time as monomers start to show up in the BN-PAGE Chromobacterium violaceum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.B16 (S)-1-phenylethylamine + pyruvate Chromobacterium violaceum
-
 acetophenone + L-alanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.B16 Chromobacterium violaceum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.B16 recombinant N-terminally His6-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and desalting gel filtration Chromobacterium violaceum

Storage Stability

EC Number Storage Stability Organism
2.6.1.B16 -20-4°C, purified recombinant enzyme in 50 mM HEPES, pH 8.2, stored for six months in 50% glycerol is still fully active (114%) and the same solution stored at room temperature maintains 77% activity Chromobacterium violaceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.B16 (S)-1-phenylethylamine + pyruvate
-
 Chromobacterium violaceum acetophenone + L-alanine
-
 r
2.6.1.B16 additional information application of the amine transaminase (ATA) for stereoselective amination of prochiral ketones represents an environmentally benign and economically attractive alternative to transition metal catalyzed asymmetric synthesis, overview Chromobacterium violaceum ?
-
-

Subunits

EC Number Subunits Comment Organism
2.6.1.B16 homodimer
-
 Chromobacterium violaceum
2.6.1.B16 More the monomer-dimer equilibrium has an effect on enzyme storage stability. After 3-4 days of incubation, the enzyme activity starts to decrease at the same time as monomers start to show up in the BN-PAGE. Cofactor has a crucial role in dimerstabilization Chromobacterium violaceum

Synonyms

EC Number Synonyms Comment Organism
2.6.1.B16 amine transaminase
-
 Chromobacterium violaceum
2.6.1.B16 ATA
-
 Chromobacterium violaceum
2.6.1.B16 Cv-ATA
-
 Chromobacterium violaceum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.B16 37
-
 assay at Chromobacterium violaceum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.B16 additional information
-
 effects of co-solvents, i.e. DMSO, glycerol, and methanol, as well as of NaCl and sucrose on the Tm value of the enzyme, overview. DMSO, NaCl, and methanol, and also phenylethylamine and L-alanine reduce the Tm value, while pyruvate, glycerol, and sucrose increase it. The addition of amine substrates (L-alanine and (S)-1-PEA) displaying destabilizing effects may result from PMP (the reduced amine form of the cofactor PLP) formation. PMP is not covalently bound to the enzyme's active site and may therefore promote enzyme destabilization or denaturation Chromobacterium violaceum
2.6.1.B16 78
-
 the enzyme displays a high Tm of 78°C in 100 mM HEPES, pH 7.4, and 100 mM NaCl Chromobacterium violaceum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.B16 8.2
-
 assay at Chromobacterium violaceum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.B16 pyridoxal 5'-phosphate PLP has a crucial role in dimerstabilization Chromobacterium violaceum