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Literature summary extracted from

  • Hengeveld, A.F.; Westphal, A.H.; de Kok, A.
    Expression and characterisation of the homodimeric E1 component of the Azotobacter vinelandii pyruvate dehydrogenase complex (1997), Eur. J. Biochem., 250, 260-268 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.104 expression in Escherichia coli Azotobacter vinelandii
1.2.4.1 expression in Escherichia coli Azotobacter vinelandii

General Stability

EC Number General Stability Organism
1.2.1.104 enzyme is stable in high ionic strength buffers Azotobacter vinelandii
1.2.1.104 enzyme is unstable in low ionic strength buffers and in the presence of organic solvents Azotobacter vinelandii
1.2.4.1 enzyme is stable in high ionic strength buffers Azotobacter vinelandii
1.2.4.1 enzyme is unstable in low ionic strength buffers and in the presence of organic solvents Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.104 acetyl-CoA product inhibition, apart from interaction with the active site on subunit E2p, acetyl CoA interacts directly with the E1p component Azotobacter vinelandii
1.2.1.104 CoA allosteric inhibition Azotobacter vinelandii
1.2.4.1 acetyl-CoA product inhibition, acetyl CoA interacts directly with the E1p component Azotobacter vinelandii
1.2.4.1 CoA allosteric inhibition Azotobacter vinelandii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.2.1.104 Ethanol 1-2%, rapid inactivation Azotobacter vinelandii
1.2.4.1 Ethanol 1-2%, rapid inactivation Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Azotobacter vinelandii V9H1F5 subunit E1p, cf. EC 1.2.4.1
-
1.2.4.1 Azotobacter vinelandii V9H1F5
-
-

Storage Stability

EC Number Storage Stability Organism
1.2.1.104 4°C, recombinant enzyme in saturated ammonium sulfate, pH 7.0, stable for 6 months Azotobacter vinelandii
1.2.4.1 4°C, recombinant enzyme in saturated ammonium sulfate, pH 7.0, stabel for 6 months Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.104 additional information non-complex-bound E1p shows the same cooperativity as found for complex-bound E1p. A Hill coefficient of 1.2-1.6 is calculated for non-complex bound E1p Azotobacter vinelandii ?
-
-
1.2.1.104 pyruvate + CoA + ferricyanide
-
 Azotobacter vinelandii acetyl-CoA + CO2 + ferrocyanide
-
 ?
1.2.4.1 additional information non-complex-bound E1p shows the same cooperativity as found for complex-bound E1p in the pyruvate dehydrogenase complex, EC 1.2.1.104. A Hill coefficient of 1.2-1.6 is calculated for non-complex bound E1p Azotobacter vinelandii ?
-
-
1.2.4.1 pyruvate + CoA + ferricyanide
-
 Azotobacter vinelandii acetyl-CoA + CO2 + ferrocyanide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.104 PdhE
-
 Azotobacter vinelandii
1.2.4.1 PdhE
-
 Azotobacter vinelandii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.104 9.5
-
 15 min, 60% residual activity Azotobacter vinelandii
1.2.4.1 9.5
-
 15 min, 60% residual activity Azotobacter vinelandii