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Literature summary extracted from

  • Rojanarata, T.; Laiwattanapaisal, W.; Ngawhirunpat, T.; Opanasopit, P.; Worrawethanakul, K.; Chantadee, T.; Fuangwattana, T.
    Fast, affordable and eco-friendly enzyme kinetic method for the assay of alpha-ketoglutaric acid in medical product and sports supplements (2018), Enzyme Microb. Technol., 116, 72-76 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.72 analysis development of a simple, inexpensive, accurate and precise kinetic method for the quantitation of 2-oxoglutarate (AKG) in cardioplegic solution and athletic supplements. The assay relies on an enzymatic transamination of AKG and D-4-hydroxyphenylglycine to form 4-hydroxybenzoylformate and L-glutamate using D-phenylglycine aminotransferase. The method shows good linearity (r2 = 0.9994) over an AKG concentration range of 0.020-0.160 mM. The limits of detection and quantitation are 0.00409 and 0.01362 mM, respectively. The detection is not interfered with by excipients in the samples Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.72 recombinant expression of His-tagged enzyme in Escherichia coli Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.72 D-4-hydroxyphenylglycine + 2-oxoglutarate Pseudomonas putida
-
 4-hydroxyphenylglyoxylate + L-glutamate
-
 ?
2.6.1.72 D-phenylglycine + 2-oxoglutarate Pseudomonas putida
-
 phenylglyoxylate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.72 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.72 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.72 D-4-hydroxyphenylglycine + 2-oxoglutarate
-
 Pseudomonas putida 4-hydroxyphenylglyoxylate + L-glutamate
-
 ?
2.6.1.72 D-phenylglycine + 2-oxoglutarate
-
 Pseudomonas putida phenylglyoxylate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.72 D-phenylglycine aminotransferase
-
 Pseudomonas putida
2.6.1.72 D-PhgAT
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.72 25
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.72 9.5
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.72 pyridoxal 5'-phosphate
-
 Pseudomonas putida