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Literature summary extracted from

  • Kim, E.M.; Park, J.H.; Kim, B.G.; Seo, J.H.
    Identification of (R)-selective omega-aminotransferases by exploring evolutionary sequence space (2018), Enzyme Microb. Technol., 110, 46-52 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.B21 gene ilvE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Evansella cellulosilytica
2.6.1.B21 gene ilvE1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus thuringiensis
2.6.1.B21 gene pabC, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Saccharopolyspora erythraea
2.6.1.B21 sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Saccharopolyspora erythraea
2.6.1.B21 sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus thuringiensis
2.6.1.B21 sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Evansella cellulosilytica
2.6.1.42 gene ilvE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Evansella cellulosilytica
2.6.1.42 gene ilvE1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate Saccharopolyspora erythraea
-
 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate Bacillus thuringiensis
-
 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica
-
 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica FERM P-1141
-
 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica ATCC 21833
-
 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica DSM 2522
-
 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica JCM 9156
-
 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate Evansella cellulosilytica N-4
-
 acetophenone + D-alanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.B21 Bacillus thuringiensis
-
-
-
2.6.1.B21 Bacillus thuringiensis A0A1B1L2T7 serovar berliner ATCC 10792
-
2.6.1.B21 Evansella cellulosilytica
-
-
-
2.6.1.B21 Evansella cellulosilytica E6TUA8
-
-
2.6.1.B21 Evansella cellulosilytica ATCC 21833
-
-
-
2.6.1.B21 Evansella cellulosilytica ATCC 21833 E6TUA8
-
-
2.6.1.B21 Evansella cellulosilytica DSM 2522
-
-
-
2.6.1.B21 Evansella cellulosilytica DSM 2522 E6TUA8
-
-
2.6.1.B21 Evansella cellulosilytica FERM P-1141
-
-
-
2.6.1.B21 Evansella cellulosilytica FERM P-1141 E6TUA8
-
-
2.6.1.B21 Evansella cellulosilytica JCM 9156
-
-
-
2.6.1.B21 Evansella cellulosilytica JCM 9156 E6TUA8
-
-
2.6.1.B21 Evansella cellulosilytica N-4
-
-
-
2.6.1.B21 Evansella cellulosilytica N-4 E6TUA8
-
-
2.6.1.B21 Saccharopolyspora erythraea
-
-
-
2.6.1.B21 Saccharopolyspora erythraea A4FQE5
-
-
2.6.1.B21 Saccharopolyspora erythraea ATCC 11635 A4FQE5
-
-
2.6.1.B21 Saccharopolyspora erythraea DSM 40517 A4FQE5
-
-
2.6.1.B21 Saccharopolyspora erythraea JCM 4748 A4FQE5
-
-
2.6.1.B21 Saccharopolyspora erythraea NBRC 13426 A4FQE5
-
-
2.6.1.B21 Saccharopolyspora erythraea NCIMB 8594 A4FQE5
-
-
2.6.1.B21 Saccharopolyspora erythraea NRRL 2338 A4FQE5
-
-
2.6.1.42 Bacillus thuringiensis A0A1B1L2T7 serovar berliner ATCC 10792
-
2.6.1.42 Evansella cellulosilytica E6TUA8
-
-
2.6.1.42 Evansella cellulosilytica ATCC 21833 E6TUA8
-
-
2.6.1.42 Evansella cellulosilytica DSM 2522 E6TUA8
-
-
2.6.1.42 Evansella cellulosilytica FERM P-1141 E6TUA8
-
-
2.6.1.42 Evansella cellulosilytica JCM 9156 E6TUA8
-
-
2.6.1.42 Evansella cellulosilytica N-4 E6TUA8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.B21 recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Saccharopolyspora erythraea
2.6.1.B21 recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Bacillus thuringiensis
2.6.1.B21 recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Evansella cellulosilytica
2.6.1.B21 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Evansella cellulosilytica
2.6.1.B21 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Bacillus thuringiensis
2.6.1.B21 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Saccharopolyspora erythraea
2.6.1.42 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Evansella cellulosilytica
2.6.1.42 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Bacillus thuringiensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.B21 0.000087
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate Bacillus thuringiensis
2.6.1.B21 0.000087
-
 purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication Bacillus thuringiensis
2.6.1.B21 0.00022
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate Bacillus thuringiensis
2.6.1.B21 0.00022
-
 purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication Bacillus thuringiensis
2.6.1.B21 0.00027
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate Evansella cellulosilytica
2.6.1.B21 0.00027
-
 purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication Evansella cellulosilytica
2.6.1.B21 0.00042
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate Evansella cellulosilytica
2.6.1.B21 0.00042
-
 purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication Evansella cellulosilytica
2.6.1.B21 0.0014
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate Evansella cellulosilytica
2.6.1.B21 0.0014
-
 purified recombinant enzyme, substrates L-leucine and 2-oxoglutarate, pH and temperature not specified in the publication Evansella cellulosilytica
2.6.1.B21 0.0015
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate Bacillus thuringiensis
2.6.1.B21 0.0015
-
 purified recombinant enzyme, substrates L-leucine and 2-oxoglutarate, pH and temperature not specified in the publication Bacillus thuringiensis
2.6.1.B21 0.003
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates D-alanine and 2-oxoglutarate Saccharopolyspora erythraea
2.6.1.B21 0.003
-
 purified recombinant enzyme, substrates D-alanine and 2-oxoglutarate, pH and temperature not specified in the publication Saccharopolyspora erythraea
2.6.1.B21 0.046
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate Saccharopolyspora erythraea
2.6.1.B21 0.046
-
 purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate, pH and temperature not specified in the publication Saccharopolyspora erythraea
2.6.1.B21 0.09
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate Saccharopolyspora erythraea
2.6.1.B21 0.09
-
 purified recombinant enzyme, substrates (R)-alpha-methylbenzylamine and pyruvate, pH and temperature not specified in the publication Saccharopolyspora erythraea
2.6.1.42 0.000087
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate Bacillus thuringiensis
2.6.1.42 0.00022
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate Bacillus thuringiensis
2.6.1.42 0.00027
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate Evansella cellulosilytica
2.6.1.42 0.00042
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate Evansella cellulosilytica
2.6.1.42 0.0014
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate Evansella cellulosilytica
2.6.1.42 0.0015
-
 purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate
-
 Saccharopolyspora erythraea acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate
-
 Bacillus thuringiensis acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate
-
 Evansella cellulosilytica acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity Evansella cellulosilytica acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity Bacillus thuringiensis acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate
-
 Evansella cellulosilytica FERM P-1141 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea ATCC 11635 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea NBRC 13426 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate
-
 Evansella cellulosilytica ATCC 21833 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea JCM 4748 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea NRRL 2338 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea NCIMB 8594 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate
-
 Evansella cellulosilytica DSM 2522 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate
-
 Evansella cellulosilytica JCM 9156 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate low activity Saccharopolyspora erythraea DSM 40517 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + 2-oxoglutarate
-
 Evansella cellulosilytica N-4 acetophenone + D-glutamate
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Saccharopolyspora erythraea acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Bacillus thuringiensis acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Evansella cellulosilytica acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor Evansella cellulosilytica acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor Bacillus thuringiensis acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Evansella cellulosilytica FERM P-1141 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Saccharopolyspora erythraea ATCC 11635 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Saccharopolyspora erythraea NBRC 13426 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Evansella cellulosilytica ATCC 21833 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Saccharopolyspora erythraea JCM 4748 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Saccharopolyspora erythraea NRRL 2338 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Saccharopolyspora erythraea NCIMB 8594 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Evansella cellulosilytica DSM 2522 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Evansella cellulosilytica JCM 9156 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Saccharopolyspora erythraea DSM 40517 acetophenone + D-alanine
-
 r
2.6.1.B21 (R)-alpha-methylbenzylamine + pyruvate
-
 Evansella cellulosilytica N-4 acetophenone + D-alanine
-
 r
2.6.1.B21 D-alanine + 2-oxoglutarate
-
 Saccharopolyspora erythraea pyruvate + D-glutamate
-
 r
2.6.1.B21 D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea pyruvate + D-glutamate
-
 r
2.6.1.B21 D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea ATCC 11635 pyruvate + D-glutamate
-
 r
2.6.1.B21 D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea NBRC 13426 pyruvate + D-glutamate
-
 r
2.6.1.B21 D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea JCM 4748 pyruvate + D-glutamate
-
 r
2.6.1.B21 D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea NRRL 2338 pyruvate + D-glutamate
-
 r
2.6.1.B21 D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea NCIMB 8594 pyruvate + D-glutamate
-
 r
2.6.1.B21 D-alanine + 2-oxoglutarate very low activity, reaction of EC 2.6.1.21 Saccharopolyspora erythraea DSM 40517 pyruvate + D-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Bacillus thuringiensis 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Bacillus thuringiensis 4-methyl-2-oxopentanoate + D-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica 4-methyl-2-oxopentanoate + D-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica FERM P-1141 4-methyl-2-oxopentanoate + D-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica ATCC 21833 4-methyl-2-oxopentanoate + D-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica DSM 2522 4-methyl-2-oxopentanoate + D-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica JCM 9156 4-methyl-2-oxopentanoate + D-glutamate
-
 r
2.6.1.B21 L-leucine + 2-oxoglutarate reaction of EC 2.6.1.42 Evansella cellulosilytica N-4 4-methyl-2-oxopentanoate + D-glutamate
-
 r
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bthu also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bthu shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Bacillus thuringiensis ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea ?
-
-
2.6.1.B21 additional information the forward reaction is preferred. Enzyme R-omegaAT_Bthu mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Bacillus thuringiensis ?
-
-
2.6.1.B21 additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica ?
-
-
2.6.1.B21 additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses DAT activity (EC 2.6.1.21). No activity with (S)-alpha-methylbenzylamine and L-leucine Saccharopolyspora erythraea ?
-
-
2.6.1.B21 additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica FERM P-1141 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica FERM P-1141 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea ATCC 11635 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea NBRC 13426 ?
-
-
2.6.1.B21 additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica ATCC 21833 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica ATCC 21833 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea JCM 4748 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea NRRL 2338 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea NCIMB 8594 ?
-
-
2.6.1.B21 additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica DSM 2522 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica DSM 2522 ?
-
-
2.6.1.B21 additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica JCM 9156 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica JCM 9156 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Sery also possesses D-alanine aminotransferase (DAT, EC 2.6.1.21) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. No BCAT activity (EC 2.6.1.42) is observed for enzyme R-omegaAT_Sery, no activity with L-leucine Saccharopolyspora erythraea DSM 40517 ?
-
-
2.6.1.B21 additional information the forward reaction is preferred. Enzyme R-omegaAT_Sery mainly possesses BCAT activity (EC 2.6.1.42). No activity with (S)-alpha-methylbenzylamine and D-alanine Evansella cellulosilytica N-4 ?
-
-
2.6.1.B21 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows very low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica N-4 ?
-
-
2.6.1.42 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica acetophenone + D-glutamate
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Bacillus thuringiensis acetophenone + D-glutamate
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica FERM P-1141 acetophenone + D-glutamate
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica ATCC 21833 acetophenone + D-glutamate
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica DSM 2522 acetophenone + D-glutamate
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica JCM 9156 acetophenone + D-glutamate
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + 2-oxoglutarate very low activity, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica N-4 acetophenone + D-glutamate
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor with (R)-alpha-methylbenzylamine, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica acetophenone + D-alanine
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Bacillus thuringiensis acetophenone + D-alanine
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor with (R)-alpha-methylbenzylamine, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica FERM P-1141 acetophenone + D-alanine
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor with (R)-alpha-methylbenzylamine, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica ATCC 21833 acetophenone + D-alanine
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor with (R)-alpha-methylbenzylamine, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica DSM 2522 acetophenone + D-alanine
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor with (R)-alpha-methylbenzylamine, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica JCM 9156 acetophenone + D-alanine
-
 r
2.6.1.42 (R)-alpha-methylbenzylamine + pyruvate the enzyme prefers pyruvate as the amino acceptor with (R)-alpha-methylbenzylamine, reaction of (R)-selective omega-aminotransferase, EC 2.6.1. Evansella cellulosilytica N-4 acetophenone + D-alanine
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Evansella cellulosilytica 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Bacillus thuringiensis 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Evansella cellulosilytica FERM P-1141 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Evansella cellulosilytica ATCC 21833 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Evansella cellulosilytica DSM 2522 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Evansella cellulosilytica JCM 9156 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 L-leucine + 2-oxoglutarate
-
 Evansella cellulosilytica N-4 4-methyl-2-oxopentanoate + L-glutamate
-
 r
2.6.1.42 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica ?
-
-
2.6.1.42 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bthu also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bthu shows low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Bacillus thuringiensis ?
-
-
2.6.1.42 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica FERM P-1141 ?
-
-
2.6.1.42 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica ATCC 21833 ?
-
-
2.6.1.42 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica DSM 2522 ?
-
-
2.6.1.42 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica JCM 9156 ?
-
-
2.6.1.42 additional information besides R-omegaAT activity, the enzyme R-omegaAT_Bcel also possesses BCAT (EC 2.6.1.42) activity. The enzyme shows no activity with (S)-alpha-methylbenzylamine. Enzyme R-omegaAT_Bcel shows low R-omegaAT activity. The enzyme does not show any D-alanine aminotransferase (DAT, EC 2.6.1.21) activity Evansella cellulosilytica N-4 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.6.1.B21 (R)-selective omega-aminotransferase
-
 Saccharopolyspora erythraea
2.6.1.B21 (R)-selective omega-aminotransferase
-
 Bacillus thuringiensis
2.6.1.B21 (R)-selective omega-aminotransferase
-
 Evansella cellulosilytica
2.6.1.B21 IlvE
-
 Evansella cellulosilytica
2.6.1.B21 ilvE1
-
 Bacillus thuringiensis
2.6.1.B21 More see also EC 2.6.1.21 Saccharopolyspora erythraea
2.6.1.B21 More see also EC 2.6.1.42 Bacillus thuringiensis
2.6.1.B21 More see also EC 2.6.1.42 Evansella cellulosilytica
2.6.1.B21 PabC
-
 Saccharopolyspora erythraea
2.6.1.B21 R-omegaAT
-
 Saccharopolyspora erythraea
2.6.1.B21 R-omegaAT
-
 Bacillus thuringiensis
2.6.1.B21 R-omegaAT
-
 Evansella cellulosilytica
2.6.1.B21 R-omegaAT_Bcel
-
 Evansella cellulosilytica
2.6.1.B21 R-omegaAT_Bthu
-
 Bacillus thuringiensis
2.6.1.B21 R-omegaAT_Sery
-
 Saccharopolyspora erythraea
2.6.1.42 (R)-selective omega-aminotransferase
-
 Evansella cellulosilytica
2.6.1.42 (R)-selective omega-aminotransferase
-
 Bacillus thuringiensis
2.6.1.42 IlvE
-
 Evansella cellulosilytica
2.6.1.42 ilvE1
-
 Bacillus thuringiensis
2.6.1.42 More see also EC 2.6.1. Evansella cellulosilytica
2.6.1.42 More see also EC 2.6.1. Bacillus thuringiensis
2.6.1.42 R-omegaAT
-
 Evansella cellulosilytica
2.6.1.42 R-omegaAT
-
 Bacillus thuringiensis
2.6.1.42 R-omegaAT_Bcel
-
 Evansella cellulosilytica
2.6.1.42 R-omegaAT_Bthu
-
 Bacillus thuringiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.B21 7
-
 assay at Evansella cellulosilytica
2.6.1.B21 7
-
 assay at Bacillus thuringiensis
2.6.1.B21 7
-
 assay at Saccharopolyspora erythraea
2.6.1.42 7
-
 assay at Evansella cellulosilytica
2.6.1.42 7
-
 assay at Bacillus thuringiensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.B21 pyridoxal 5'-phosphate
-
 Saccharopolyspora erythraea
2.6.1.B21 pyridoxal 5'-phosphate
-
 Bacillus thuringiensis
2.6.1.B21 pyridoxal 5'-phosphate
-
 Evansella cellulosilytica
2.6.1.B21 pyridoxal 5'-phosphate PLP, dependent on Evansella cellulosilytica
2.6.1.B21 pyridoxal 5'-phosphate PLP, dependent on Bacillus thuringiensis
2.6.1.B21 pyridoxal 5'-phosphate PLP, dependent on Saccharopolyspora erythraea
2.6.1.42 pyridoxal 5'-phosphate PLP, dependent on Evansella cellulosilytica
2.6.1.42 pyridoxal 5'-phosphate PLP, dependent on Bacillus thuringiensis

General Information

EC Number General Information Comment Organism
2.6.1.B21 evolution (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III Saccharopolyspora erythraea
2.6.1.B21 evolution (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III Bacillus thuringiensis
2.6.1.B21 evolution (R)-selective omega-aminotransferases (R-omegaATs) are variants of aminotransferase group III Evansella cellulosilytica
2.6.1.B21 evolution the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G Evansella cellulosilytica
2.6.1.B21 evolution the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G Bacillus thuringiensis
2.6.1.B21 evolution the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G Saccharopolyspora erythraea
2.6.1.42 evolution the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G Evansella cellulosilytica
2.6.1.42 evolution the enzyme belongs to the PLP fold type IV transaminases. PLP fold type IV transaminases include branched-chain amino acid transaminases (BCATs), D-amino acid transaminases, and (R)-amine:pyruvate transaminases. It is generally accepted that R-omegaATs are variants of aminotransferase group III. Library screening, phylogenetic analysis. R-omegaAT enzyme secondary structure and structural motifs comparisons, overview. V238I variation is observed among residues in PLP binding site. Val62 and Thr274 are changed to glycine in Bacillus cellulosilyticus R-omegaAT_Bcel and Bacillus thuringiensis R-omegaAT_Bthu among residues in the small binding pocket. H55Y, Y60F, F115Y, E117R, and W184Y variations and deletion of R128 are observed among residues in the large binding pocket. Noticeable variation include the deletion of Arg128 and variation of V62G and T274G Bacillus thuringiensis