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Literature summary extracted from

  • Torretta, A.; Lopez-Cara, L.; Parisini, E.
    Crystal structure of the apo and the adp-bound form of choline kinase from Plasmodium falciparum (2020), Crystals, 10, 1-13 .
No PubMed abstract available

Application

EC Number Application Comment Organism
2.7.1.32 drug development targeting the parasite lipid metabolism and choline kinase for pharmacological action constitutes an effective way of dealing with the spreading of the disease and with conventional therapy-resistant strains Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Star Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.32 purified recombinat His6-tagged choline kinase in apoform and in ADP-bound form, hanging drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein solution with 0.001 ml of reservoir solution, and equilibration against 0.5 ml reservoir solution, containing 16% v/v PEG 8000, 0.2 M NaCl, 0.1 M HEPES, pH 7.5, 2 mM Tris(2-carboxyethyl)phosphine (TCEP), and 4 mM MgCl2 for the apoform crystals, and the same composition with 2 mM ADP for the complex crystals, at room temperature, X-ray diffraction structrue determination and analysis at 2.0-2.2 A resolution, molecular replacement and model building using the PDB ID 3FI8 structure as starting model Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Mg2+ required, enzyme binding structure analysis. The ADP molecule also participates in the coordination of two magnesium ions Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.32 ATP + choline Plasmodium falciparum
-
 ADP + phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Plasmodium falciparum Q8IM71
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) Star by nickel affinity chromatography and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Plasmodium falciparum ADP + phosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 PfChoK
-
 Plasmodium falciparum

General Information

EC Number General Information Comment Organism
2.7.1.32 malfunction selective inhibition of the enzyme and the Kennedy pathway leads to the parasite arrest and eradication Plasmodium falciparum
2.7.1.32 metabolism within the Kennedy pathway, the Plasmodium falciparum choline kinase (PfChoK) has a central role in the biosynthesis of phosphatidylcholine. Plasmodium falciparum plasma membrane is mainly composed by phosphatidylcholine (PC) and phosphatidylethanolamine (PE), which represent 40-50% and 35-40% of the total phospholipid content, respectively Plasmodium falciparum
2.7.1.32 additional information substrate binding structure analysis, overview Plasmodium falciparum