Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sinitsyna, E.; Timofeev, V.; Tuzova, E.; Kostromina, M.; Murav'eva, T.; Esipov, R.; Kuranova, I.
    Crystallization and preliminary X-ray diffraction study of recombinant adenine phosphoribosyltransferase from the thermophilic bacterium Thermus thermophilus strain HB27 (2017), Crystallogr. Rep., 62, 580-583 .
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.7 purified recombinant enzyme, hanging drop vapor diffusion technique, mixing of 12.5 mg/ml protein in 0.02 M Tris-HCl buffer, pH 8.0, 50 mM NaCl, 5% glycerol, 0.04% NaN3, and 5 mM AMP, with reservoir solution composed of 25% w/v PEG 3350, 0.1 M HEPES, pH 7.0, 0.5 M NaCl, and 0.04% NaN3, X-ray diffraction structure determination and analysis at 2.60 A resolution Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus
-
 AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus DSM 7039
-
 AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus ATCC BAA-163
-
 AMP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Thermus thermophilus Q72I82
-
-
2.4.2.7 Thermus thermophilus ATCC BAA-163 Q72I82
-
-
2.4.2.7 Thermus thermophilus DSM 7039 Q72I82
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7 native enzyme by heat treatment for 10 min at 65°C, desalting gel filtration, anion exchange and hydrophobic interaction chromatography, ultrafiltration, and gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 2,6-diaminpurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 2,6-diaminpurine 5'-phosphoribosyl nucleotide + diphosphate
-
 r
2.4.2.7 2,6-diaminpurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 2,6-diaminpurine 5'-phosphoribosyl nucleotide + diphosphate
-
 r
2.4.2.7 2,6-diaminpurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 2,6-diaminpurine 5'-phosphoribosyl nucleotide + diphosphate
-
 r
2.4.2.7 2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 2-chloro-AMP + diphosphate
-
 r
2.4.2.7 2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 2-chloro-AMP + diphosphate
-
 r
2.4.2.7 2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 2-chloro-AMP + diphosphate
-
 r
2.4.2.7 2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 2-fluoro-AMP + diphosphate
-
 r
2.4.2.7 2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 2-fluoro-AMP + diphosphate
-
 r
2.4.2.7 2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 2-fluoro-AMP + diphosphate
-
 r
2.4.2.7 2-methoxyadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 2-methoxyadenosine 5'-monophosphate + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 AMP + diphosphate
-
 r
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 AMP + diphosphate
-
 r
2.4.2.7 additional information not only adenine but also a number of its derivatives, including 2-chloro- and 2-fluoroadenine, 2-methoxyadenine, N1-methoxyadenine, and 2,6-diaminpurine, are substrates of the enzyme Thermus thermophilus ?
-
-
2.4.2.7 additional information not only adenine but also a number of its derivatives, including 2-chloro- and 2-fluoroadenine, 2-methoxyadenine, N1-methoxyadenine, and 2,6-diaminpurine, are substrates of the enzyme Thermus thermophilus DSM 7039 ?
-
-
2.4.2.7 additional information not only adenine but also a number of its derivatives, including 2-chloro- and 2-fluoroadenine, 2-methoxyadenine, N1-methoxyadenine, and 2,6-diaminpurine, are substrates of the enzyme Thermus thermophilus ATCC BAA-163 ?
-
-
2.4.2.7 N1-methoxyadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus N1-methoxyadenosine 5'-monophosphate + diphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.2.7 APRT
-
 Thermus thermophilus
2.4.2.7 TthAPRT
-
 Thermus thermophilus
2.4.2.7 TT_C1250 locus name Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 73
-
 recombinant enzyme Thermus thermophilus

General Information

EC Number General Information Comment Organism
2.4.2.7 physiological function adenine phosphoribosyltransferase (APRT) belongs to the type I phosphoribosyltransferases and catalyzes the formation of adenosine monophosphate via transfer of the 5-phosphoribosyl group from phosphoribosyl diphosphate to the nitrogen atom N9 of the adenine base Thermus thermophilus