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Literature summary extracted from

  • Pelot, K.A.; Hagelthorn, D.M.; Hong, Y.J.; Tantillo, D.J.; Zerbe, P.
    Diterpene synthase-catalyzed biosynthesis of distinct clerodane stereoisomers (2020), Chembiochem, 20, 111-117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.B7 expressed in Escherichia coli Panicum virgatum

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.B7 F251A the mutant produces (5S,8S,9R,10R)-13Z-clerodienyl diphosphate from (Z,Z,Z)-nerylneryl diphosphate with a 3fold increased activity compared to the wild type enzyme. The enzyme also produces cis,cis-clerodienyl diphosphate besides the natural product cis,trans-clerodienyl diphosphate from (E,E,E)-geranylgeranyl diphosphate Panicum virgatum
5.5.1.B7 F251G the mutant shows reduced activity towards (E,E,E)-geranylgeranyl diphosphate compared to the wild type enzyme Panicum virgatum
5.5.1.B7 F251S the mutant shows reduced activity towards (E,E,E)-geranylgeranyl diphosphate compared to the wild type enzyme Panicum virgatum
5.5.1.B7 F251V the mutant produces (5S,8S,9R,10R)-13Z-clerodienyl diphosphate from (Z,Z,Z)-nerylneryl diphosphate with an 8fold increased activity compared to the wild type enzyme. The enzyme also produces cis,cis-clerodienyl diphosphate besides the natural product cis,trans-clerodienyl diphosphate from (E,E,E)-geranylgeranyl diphosphate Panicum virgatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.142 dimethylallyl diphosphate + 3 isopentenyl diphosphate Solanum lycopersicum
-
 3 diphosphate + nerylneryl diphosphate
-
 ?
5.5.1.B7 (E,E,E)-geranylgeranyl diphosphate Panicum virgatum
-
 cis,trans-clerodienyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.142 Solanum lycopersicum
-
-
-
5.5.1.B7 Panicum virgatum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.142 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Solanum lycopersicum 3 diphosphate + nerylneryl diphosphate
-
 ?
5.5.1.B7 (E,E,E)-geranylgeranyl diphosphate
-
 Panicum virgatum cis,trans-clerodienyl diphosphate
-
 ?
5.5.1.B7 (Z,Z,Z)-nerylneryl diphosphate
-
 Panicum virgatum (5S,8S,9R,10R)-13Z-clerodienyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.142 NNPP synthase
-
 Solanum lycopersicum
5.5.1.B7 CPS1
-
 Panicum virgatum