Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, K.; Parmeggiani, F.; Pallister, E.; Huang, C.J.; Liu, F.F.; Li, Q.; Birmingham, W.R.; Both, P.; Thomas, B.; Liu, L.; Voglmeir, J.; Flitsch, S.L.
    Characterisation of a bacterial galactokinase with high activity and broad substrate tolerance for chemoenzymatic synthesis of 6-aminogalactose-1-phosphate and analogues (2018), ChemBioChem, 19, 388-394 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.6 synthesis LgGalK and galactose oxidase variant M1 are combined in a one-pot, two-step system to synthesize 6-oxogalactose-1-phosphate and 6-oxo-2-fluorogalactose-1-phosphate, which are subsequently used to produce a panel of 30 substituted 6-aminogalactose-1-phosphate derivatives by chemical reductive amination in a one-pot, three-step chemoenzymatic process Leminorella grimontii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.6 expression in Escherichia coli Leminorella grimontii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.6 Cu2+
-
 Leminorella grimontii
2.7.1.6 EDTA
-
 Leminorella grimontii
2.7.1.6 Zn2+
-
 Leminorella grimontii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 1.3
-
 alpha-D-galactose pH 9.0, 50°C Leminorella grimontii
2.7.1.6 1.5
-
 ATP pH 9.0, 50°C Leminorella grimontii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.6 Ca2+ activates Leminorella grimontii
2.7.1.6 Fe2+ activates Leminorella grimontii
2.7.1.6 Mg2+ activates. Optimum Mg2+ concentration is around 1 mm, with a very modest decrease in activity as the Mg2+ concentration increases Leminorella grimontii
2.7.1.6 Ni2+ activates Leminorella grimontii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.6 40000
-
 SDS-PAGE Leminorella grimontii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Leminorella grimontii A0A085HKM4
-
-
2.7.1.6 Leminorella grimontii DSM 5078 A0A085HKM4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6
-
 Leminorella grimontii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + 3-deoxy-3-fluorogalactose 27% conversion Leminorella grimontii ADP + 3-deoxy-3-fluorogalactose 1-phosphate
-
 ?
2.7.1.6 ATP + 3-deoxy-3-fluorogalactose 27% conversion Leminorella grimontii DSM 5078 ADP + 3-deoxy-3-fluorogalactose 1-phosphate
-
 ?
2.7.1.6 ATP + 3-deoxy-D-galactose 19% conversion Leminorella grimontii ADP + 3-deoxy-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + 4-deoxy-4-fluorogalactose 21% conversion Leminorella grimontii ADP + 4-deoxy-4-fluorogalactose 1-phosphate
-
 ?
2.7.1.6 ATP + 6-deoxy-6-fluorogalactose 28% conversion Leminorella grimontii ADP + 6-deoxy-6-fluorogalactose 1-phosphate
-
 ?
2.7.1.6 ATP + 6-deoxy-D-galactose 57% conversion Leminorella grimontii ADP + 6-deoxy-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + 6-deoxy-D-galactose 57% conversion Leminorella grimontii DSM 5078 ADP + 6-deoxy-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + alpha-D-galactose 72% conversion Leminorella grimontii ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + alpha-D-galactose 72% conversion Leminorella grimontii DSM 5078 ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactosamine 68% conversion Leminorella grimontii ADP + alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.6 ATP + D-galactosamine 68% conversion Leminorella grimontii DSM 5078 ADP + alpha-D-galactosamine 1-phosphate
-
 ?
2.7.1.6 ATP + D-talose 41% conversion Leminorella grimontii ADP + alpha-D-talose 1-phosphate
-
 ?
2.7.1.6 ATP + D-talose 41% conversion Leminorella grimontii DSM 5078 ADP + alpha-D-talose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.6 ? x * 40000, SDS-PAGE Leminorella grimontii
2.7.1.6 ? x * 42000, calculated from sequence Leminorella grimontii

Synonyms

EC Number Synonyms Comment Organism
2.7.1.6 GALK
-
 Leminorella grimontii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.6 50
-
-
 Leminorella grimontii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.6 15 60 15°C: about 55% of maximal activity, 60°C: about 70% of maximal activity Leminorella grimontii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.6 2 3.7 alpha-D-galactose pH 9.0, 50°C Leminorella grimontii
2.7.1.6 21.3
-
 ATP pH 9.0, 50°C Leminorella grimontii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.6 9
-
-
 Leminorella grimontii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.6 7 10 pH 7.0: about 65% of maximal activity, pH 10.0: about 90% of maximal activity Leminorella grimontii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.6 18.1
-
 alpha-D-galactose pH 9.0, 50°C Leminorella grimontii
2.7.1.6 32.9
-
 ATP pH 9.0, 50°C Leminorella grimontii