Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sun, H.; Zhao, H.; Ang, E.
    A coupled chlorinase-fluorinase system with a high efficiency of trans-halogenation and a shared substrate tolerance (2018), Chem. Commun. (Camb.), 54, 9458-9461 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.63 synthesis deveopment of a coupled chlorinase-fluorinase system for rapid transhalogenation. The chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5'-fluorodeoxy-2-ethynyladenosine (5'-FDEA) in up to 91.6% yield in 1 h Streptomyces sp. MA37
2.5.1.94 synthesis deveopment of a coupled chlorinase-fluorinase system for rapid transhalogenation. The chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5'-fluorodeoxy-2-ethynyladenosine in up to 91.6% yield in 1 h Streptomyces albulus
2.5.1.94 synthesis deveopment of a coupled chlorinase-fluorinase system for rapid transhalogenation. The chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5'-fluorodeoxy-2-ethynyladenosine in up to 91.6% yield in 1 h Umezawaea tangerina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.94 expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.94 0.00248
-
 S-adenosyl-L-methionine isoform ClA2, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 0.0025
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Umezawaea tangerina
2.5.1.94 0.00945
-
 5-deoxy-5-chloroadenosine isoform ClA2, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 0.0095
-
 5-deoxy-5'-chloroadenosine pH not specified in the publication, temperature not specified in the publication Umezawaea tangerina
2.5.1.94 0.01398
-
 5-deoxy-5-chloroadenosine isoform ClA1, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 0.014
-
 5-deoxy-5'-chloroadenosine pH not specified in the publication, temperature not specified in the publication Streptomyces albulus
2.5.1.94 0.01569
-
 S-adenosyl-L-methionine isoform ClA1, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 0.0157
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces albulus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.94 5-deoxy-5-chloroadenosine + L-methionine uncultured bacterium most efficient reaction S-adenosyl-L-methionine + chloride
-
 r
2.5.1.94 S-adenosyl-L-methionine + chloride uncultured bacterium
-
 5-deoxy-5-chloroadenosine + L-methionine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces sp. MA37 W0W999
-
-
2.5.1.94 Streptomyces albulus
-
-
-
2.5.1.94 Umezawaea tangerina
-
-
-
2.5.1.94 Umezawaea tangerina NRRL B-24463
-
-
-
2.5.1.94 uncultured bacterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.94 TALON metal affinity resin column chromatography uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 2'-deoxy-2-ethynyladenosyl-L-methionine + fluoride
-
 Streptomyces sp. MA37 2'-deoxy-2-ethynyl-5'-fluoroadenosine + L-methionine
-
 r
2.5.1.94 2'-deoxy-2-ethynyladenosyl-L-methionine + fluoride
-
 Streptomyces albulus 2'-deoxy-2-ethynyl-5'-fluoroadenosine + L-methionine
-
 r
2.5.1.94 2'-deoxy-2-ethynyladenosyl-L-methionine + fluoride
-
 Umezawaea tangerina 2'-deoxy-2-ethynyl-5'-fluoroadenosine + L-methionine
-
 r
2.5.1.94 2'-deoxy-2-ethynyladenosyl-L-methionine + fluoride
-
 Umezawaea tangerina NRRL B-24463 2'-deoxy-2-ethynyl-5'-fluoroadenosine + L-methionine
-
 r
2.5.1.94 5-deoxy-5'-chloroadenosine + L-methionine
-
 Streptomyces albulus S-adenosyl-L-methionine + chloride
-
 r
2.5.1.94 5-deoxy-5'-chloroadenosine + L-methionine
-
 Umezawaea tangerina S-adenosyl-L-methionine + chloride
-
 r
2.5.1.94 5-deoxy-5'-chloroadenosine + L-methionine
-
 Umezawaea tangerina NRRL B-24463 S-adenosyl-L-methionine + chloride
-
 r
2.5.1.94 5-deoxy-5-chloroadenosine + L-methionine most efficient reaction uncultured bacterium S-adenosyl-L-methionine + chloride
-
 r
2.5.1.94 S-adenosyl-L-methionine + chloride
-
 uncultured bacterium 5-deoxy-5-chloroadenosine + L-methionine
-
 r
2.5.1.94 S-adenosyl-L-methionine + chloride
-
 Streptomyces albulus 5-deoxy-5'-chloroadenosine + L-methionine
-
 r
2.5.1.94 S-adenosyl-L-methionine + chloride
-
 Umezawaea tangerina 5-deoxy-5'-chloroadenosine + L-methionine
-
 r
2.5.1.94 S-adenosyl-L-methionine + chloride
-
 Umezawaea tangerina NRRL B-24463 5-deoxy-5'-chloroadenosine + L-methionine
-
 r

Subunits

EC Number Subunits Comment Organism
2.5.1.94 ? x * about 30000-35000, SDS-PAGE uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
2.5.1.94 Cla1
-
 uncultured bacterium
2.5.1.94 Cla1
-
 Streptomyces albulus
2.5.1.94 ClA2
-
 uncultured bacterium
2.5.1.94 ClA2
-
 Umezawaea tangerina
2.5.1.94 SAM-dependent chlorinase
-
 uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.94 0.011
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Umezawaea tangerina
2.5.1.94 0.011
-
 S-adenosyl-L-methionine isoform ClA2, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 0.078
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces albulus
2.5.1.94 0.078
-
 S-adenosyl-L-methionine isoform ClA1, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 0.208
-
 5-deoxy-5'-chloroadenosine pH not specified in the publication, temperature not specified in the publication Streptomyces albulus
2.5.1.94 0.208
-
 5-deoxy-5-chloroadenosine isoform ClA1, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 0.248
-
 5-deoxy-5'-chloroadenosine pH not specified in the publication, temperature not specified in the publication Umezawaea tangerina
2.5.1.94 0.249
-
 5-deoxy-5-chloroadenosine isoform ClA2, at pH 7.8 and 37°C uncultured bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.94 4.5
-
 S-adenosyl-L-methionine isoform ClA2, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 4.502
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Umezawaea tangerina
2.5.1.94 4.968
-
 S-adenosyl-L-methionine pH not specified in the publication, temperature not specified in the publication Streptomyces albulus
2.5.1.94 4.97
-
 S-adenosyl-L-methionine isoform ClA1, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 14.89
-
 5-deoxy-5'-chloroadenosine pH not specified in the publication, temperature not specified in the publication Streptomyces albulus
2.5.1.94 14.89
-
 5-deoxy-5-chloroadenosine isoform ClA1, at pH 7.8 and 37°C uncultured bacterium
2.5.1.94 26.29
-
 5-deoxy-5'-chloroadenosine pH not specified in the publication, temperature not specified in the publication Umezawaea tangerina
2.5.1.94 26.29
-
 5-deoxy-5-chloroadenosine isoform ClA2, at pH 7.8 and 37°C uncultured bacterium