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Literature summary extracted from

  • Chen, C.; Hou, X.; Utkina, N.; Danilov, L.; Zhou, D.; Torgov, V.; Veselovsky, V.; Liu, B.; Feng, L.
    Identification and biochemical characterization of a novel alpha-1,3-mannosyltransferase WfcD from Escherichia coli O141 (2017), Carbohydr. Res., 443-444, 78-86 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.132 EDTA
-
 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.132 gene wfcD, included in the O-antigen repeating unit and O-antigen biosynthesis of Escherichia coli strain O141, genetic structure of the gene cluster, overview. Functional recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.132 Co2+
-
 Escherichia coli
2.4.1.132 Cu2+
-
 Escherichia coli
2.4.1.132 Ni2+
-
 Escherichia coli
2.4.1.132 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.132 0.56
-
 GDP-alpha-D-mannose pH 7.0, 37°C, recombinant enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.132 Ca2+ activates Escherichia coli
2.4.1.132 Mg2+ activates Escherichia coli
2.4.1.132 Mn2+ activates Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.132 GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol Escherichia coli
-
 GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
 ?
2.4.1.132 GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol Escherichia coli O141
-
 GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.132 Escherichia coli A6MF03
-
-
2.4.1.132 Escherichia coli O141 A6MF03
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.132 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.132 GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
 Escherichia coli GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
 ?
2.4.1.132 GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
 Escherichia coli O141 GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
-
 ?
2.4.1.132 GDP-alpha-D-mannose + decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose i.e. synthetic acceptor GlcNAc-PP-De Escherichia coli GDP + ?
-
 ?
2.4.1.132 GDP-alpha-D-mannose + decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose i.e. synthetic acceptor GlcNAc-PP-De Escherichia coli O141 GDP + ?
-
 ?
2.4.1.132 GDP-alpha-D-mannose + P1-[11- (anthracen-9-ylmethoxy) undecyl]-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl) diphosphate i.e. GlcNAc-PP-AnthrU Escherichia coli GDP + ?
-
 ?
2.4.1.132 GDP-alpha-D-mannose + P1-[11- (anthracen-9-ylmethoxy) undecyl]-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl) diphosphate i.e. GlcNAc-PP-AnthrU Escherichia coli O141 GDP + ?
-
 ?
2.4.1.132 additional information enzyme WfcD is expressed in Escherichia coli strain BL21(DE3), and the enzymatic product is identified by liquid chromatography-mass spectrometry (LC-MS), collision-induced dissociation electrospray ionization ion trap multiple tandem MS (CID-ESI-IT-MSn), and glycosidase digestion using the donor substrate GDP-Man and the synthetic acceptor substrate decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose (GlcNAc-PP-De). Analysis of donor substrate specificity of WfcD, overview. WfcD is an alpha-1,3-ManT that catalyzes the Man(alpha1-3)-GlcNAc-R linkage formation. No activity with GlcNAc-GlcNAc-PP-De, Glc-PP-De, UDP-GlcNAc, GlcNAc, and GlcNAc-NP Escherichia coli ?
-
-
2.4.1.132 additional information enzyme WfcD is expressed in Escherichia coli strain BL21(DE3), and the enzymatic product is identified by liquid chromatography-mass spectrometry (LC-MS), collision-induced dissociation electrospray ionization ion trap multiple tandem MS (CID-ESI-IT-MSn), and glycosidase digestion using the donor substrate GDP-Man and the synthetic acceptor substrate decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose (GlcNAc-PP-De). Analysis of donor substrate specificity of WfcD, overview. WfcD is an alpha-1,3-ManT that catalyzes the Man(alpha1-3)-GlcNAc-R linkage formation. No activity with GlcNAc-GlcNAc-PP-De, Glc-PP-De, UDP-GlcNAc, GlcNAc, and GlcNAc-NP Escherichia coli O141 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.4.1.132 ? x * 44500, about, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.4.1.132 alpha-1,3-mannosyltransferase
-
 Escherichia coli
2.4.1.132 alpha-1,3-ManT
-
 Escherichia coli
2.4.1.132 WfcD
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.132 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.132 7
-
 recombinant enzyme Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.132 5 9 activity range, donor substrate specificity, profile overview Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.1.132 physiological function WfcD is the first characterized bacterial mannosyltransferase that acts on the Man-alpha-(1->3)-GlcNAc linkage Escherichia coli