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Literature summary extracted from

  • Anwar, A.; She, M.; Wang, K.; Ye, X.
    Cloning and molecular characterization of Triticum aestivum ornithine amino transferase (TaOAT) encoding genes (2020), BMC Plant Biol., 20, 187 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.13 gene TaOAT-5AL, located on chromosome 5, DNA and amino acid sequence determination and analysis, sequence alignment between genomic DNA and its corresponding cDNA obtains a total of ten exons and nine introns, the three genes have 87.68 and 87.18% identity at the gDNA and cDNA levels, respectively, genetic structure, and phylogenetic tree. Two transcript variants of TaOAT-5AL are revealed, named TaOAT-5AL-1 and TaOAT-5AL-2 and characterized by 1497 bp and 1287 bp in cDNA length, respectively. Compared to TaOAT-5AL-2, TaOAT-5AL-1 contains an additional 120-bp insertion encompassing an in-frame stop codon, which resulted in a premature protein. The additional insertion is genotypically confirmed by sequencing results from six cultivars. The TaOAT-5AL-1 gene transcript variant contains in-frame stop codon that causes the incomplete translation of protein. Recombinant overexpression of TaOAT-AL-2 in Triticum aestivum plants, recombinant expression of C-terminally GFP-tagged enzyme in Triticum aestivum plants in mitochondria, quantitative reverse transcription PCR (qRT-PCR) analysis Triticum aestivum
2.6.1.13 gene TaOAT-5BL, located on chromosome 5, DNA and amino acid sequence determination and analysis, sequence alignment between genomic DNA and its corresponding cDNA obtains a total of ten exons and nine introns, the three genes have 87.68 and 87.18% identity at the gDNA and cDNA levels, respectively, genetic structure and phylogenetic tree, recombinant overexpression in Triticum aestivum plants, recombinant expression of C-terminally GFP-tagged enzyme in triticum aestivum plants in mitochondria quantitative reverse transcription PCR (qRT-PCR) analysis Triticum aestivum
2.6.1.13 gene TaOAT-5DL, located on chromosome 5, DNA and amino acid sequence determination and analysis, sequence alignment between genomic DNA and its corresponding cDNA obtains a total of ten exons and nine introns, the three genes have 87.68 and 87.18% identity at the gDNA and cDNA levels, respectively, genetic structure and phylogenetic tree, recombinant overexpression in Triticum aestivum plants, recombinant expression of C-terminally GFP-tagged enzyme in triticum aestivum plants in mitochondria, quantitative reverse transcription PCR (qRT-PCR) analysis Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.13 mitochondrion
-
 Triticum aestivum 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.13 L-ornithine + a 2-oxo carboxylate Triticum aestivum
-
 L-glutamate 5-semialdehyde + an L-amino acid
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.13 Triticum aestivum A0A3B6KM96
-
-
2.6.1.13 Triticum aestivum A0A3B6LSQ4
-
-
2.6.1.13 Triticum aestivum A0A3B6MXE9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.13 anther low expression level Triticum aestivum
-
2.6.1.13 carpel very low expression level Triticum aestivum
-
2.6.1.13 glume moderate expression level Triticum aestivum
-
2.6.1.13 leaf moderate expression, relative expression of transcripts in leaves gradually upregulated until it peaks at heading stage and then decreases at grain filling stage Triticum aestivum
-
2.6.1.13 additional information analysis of expression profile of the wheat OAT genes in different tissues and developmental stages, overview. Expression at spike-developmental stages are strong at tipping, heading, and anthesis stages with the highest expression at the heading stage Triticum aestivum
-
2.6.1.13 palea very low expression level Triticum aestivum
-
2.6.1.13 root very low expression level Triticum aestivum
-
2.6.1.13 seed moderate expression level Triticum aestivum
-
2.6.1.13 stamen high expression level Triticum aestivum
-
2.6.1.13 stem moderate expression level Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.13 L-ornithine + a 2-oxo carboxylate
-
 Triticum aestivum L-glutamate 5-semialdehyde + an L-amino acid
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.13 deltaOAT
-
 Triticum aestivum
2.6.1.13 OAT
-
 Triticum aestivum
2.6.1.13 ornithine delta aminotransferase
-
 Triticum aestivum
2.6.1.13 TaOAT
-
 Triticum aestivum
2.6.1.13 TaOAT-5AL
-
 Triticum aestivum
2.6.1.13 TaOAT-5B
-
 Triticum aestivum
2.6.1.13 TaOAT-5BL
-
 Triticum aestivum
2.6.1.13 TaOAT-5DL
-
 Triticum aestivum
2.6.1.13 TaOAT-AL-2
-
 Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.13 pyridoxal 5'-phosphate PLP, dependent on Triticum aestivum

Expression

EC Number Organism Comment Expression
2.6.1.13 Triticum aestivum TaOAT is highly induced in the wheat plants exposed to drought or salt stress condition, through exogenous PEG and NaCl. Upregulated expression of TaOATs is observed in stamens and at the heading stage up
2.6.1.13 Triticum aestivum TaOAT is highly induced in the wheat plants exposed to drought or salt stress condition, through exogenous PEG and NaCl. Upregulated expression of TaOATs is observed in stamens and at the heading stage. TaOAT-5BL is greatly upregulated in the transgenic plants as compared to wild-type plants in response to salt stress condition up

General Information

EC Number General Information Comment Organism
2.6.1.13 evolution ornithine aminotransferase is a highly conserved enzyme present in all prokaryotes and eukaryotes, from unicellular bacteria to multicellular animals and plants. In Triticum aestivum, three homeologous OAT genes in wheat genome are found on chromosome group 5, named as TaOAT-5AL, TaOAT-5BL, and TaOAT-5DL. The phylogenetic tree indicates that OATs share highly conserved domains between monocotyledons and eudicotyledons Triticum aestivum
2.6.1.13 evolution ornithine aminotransferase is a highly conserved enzyme present in all prokaryotes and eukaryotes, from unicellular bacteria to multicellular animals and plants. In Triticum aestivum, three homeologous OAT genes in wheat genome are found on chromosome group 5, named as TaOAT-5AL, TaOAT-5BL, and TaOAT-5DL. Two transcript variants of TaOAT-5AL are revealed, named TaOAT-5AL-1 and TaOAT-5AL-2 and characterized by 1497 bp and 1287 bp in cDNA length, respectively. Compared to TaOAT-5AL-2, TaOAT-5AL-1 contains an additional 120-bp insertion encompassing an in-frame stop codon, which resulted in a premature protein. The additional insertion is genotypically confirmed by sequencing results from six cultivars. The phylogenetic tree indicates that OATs share highly conserved domains between monocotyledons and eudicotyledons Triticum aestivum
2.6.1.13 malfunction transgenic plants overexpressing TaOAT show enhanced tolerance to drought stress by increasing proline accumulation. In addition, salt tolerance of the transgenic plants is also enhanced Triticum aestivum
2.6.1.13 malfunction transgenic plants overexpressing TaOAT show enhanced tolerance to drought stress by increasing proline accumulation. In addition, salt tolerance of the transgenic plants is also enhanced. The TaOAT-5AL-1 gene transcript variant contains in-frame stop codon that causes the incomplete translation of protein Triticum aestivum
2.6.1.13 metabolism the PLP-dependent enzyme is involved in the interconversion of ornithine and glutamyl-5-semialdehyde (GSA) Triticum aestivum
2.6.1.13 physiological function the enzyme plays a role in proline biosynthesis through interactions with genes, such as delta 1-pyrroline-5-carboxylate synthetase (P5CS) and pyrroline-5-carboxylate reductase (P5CR), involved in the proline metabolic pathway, protein-protein interactions analysis, overview. Promoter analysis exposes the presence of several stress responsive elements, implying their involvement in stress regulation. Potential role of TaOAT genes during floret development. TaOATs genes are involved in proline synthesis and nitrogen remobilization because they interact with genes related to proline biosynthesis enzymes and arginine catabolism. The high expression observed in the stamen and low expression observed at the anthesis stage suggest that TaOATs are likely to be involved in anther dehiscence Triticum aestivum