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Literature summary extracted from

  • Yang, S.J.; Choi, S.J.; Park, B.R.; Kim, Y.M.
    Thermostable CITase from Thermoanaerobacter thermocopriae shows negative cooperativity (2019), Biotechnol. Lett., 41, 625-632 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.248 synthesis the enzyme can be applied to produce relatively large CIs at high temperature Thermoanaerobacter thermocopriae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.248 expressed in Escherichia coli Thermoanaerobacter thermocopriae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.248 171000
-
 SDS-PAGE Thermoanaerobacter thermocopriae
2.4.1.248 175269
-
 calculated from sequence Thermoanaerobacter thermocopriae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.248 Thermoanaerobacter thermocopriae WP_028992696
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.248
-
 Thermoanaerobacter thermocopriae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.248 dextran cycloisomaltooligosaccharide-7 to cycloisomaltooligosaccharide-20 are mainly produced, with CI-7 as the main product Thermoanaerobacter thermocopriae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.248 ? x * 171000, SDS-PAGE Thermoanaerobacter thermocopriae
2.4.1.248 ? x * 175269, calculated from sequence Thermoanaerobacter thermocopriae

Synonyms

EC Number Synonyms Comment Organism
2.4.1.248 CITase
-
 Thermoanaerobacter thermocopriae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.248 60
-
 1 h incubation Thermoanaerobacter thermocopriae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.248 40 70 40°C: about 40% of maximal activity, 70°C: about 65% of maximal activity Thermoanaerobacter thermocopriae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.248 60
-
 24 h, thermal stability of the protein is 63%. NO loss of activity after 1 h Thermoanaerobacter thermocopriae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.248 6
-
-
 Thermoanaerobacter thermocopriae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.248 5.5 7 pH 5.5: about 75% of maximal activity, pH 7.0: about 75% of maximal activity Thermoanaerobacter thermocopriae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.248 5 11 4°C, 18 h, stable Thermoanaerobacter thermocopriae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.248 67.4
-
 Dextran pH 6.0, 50°C Thermoanaerobacter thermocopriae