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Literature summary extracted from

  • Liu, K.; Zhou, Y.; Zhang, J.; Chu, J.; Zhang, Y.; He, B.
    A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated nucleosides (2017), Biotechnol. Lett., 39, 1903-1910 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.2 gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons Siminovitchia fordii
2.4.2.2 gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons Brevibacillus borstelensis
2.4.2.2 gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons Aneurinibacillus migulanus
2.4.2.2 gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons Lacticaseibacillus paracasei
2.4.2.2 gene mining using the sequence of Geobacillus stearothermophilus gene pdp (UniProt ID P77836) as search model, recombinant expression in Escherichia coli strain BL21(DE3), sequence comparisons Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.2 additional information
-
 additional information Michaelis-Menten kinetics Siminovitchia fordii
2.4.2.2 additional information
-
 additional information Michaelis-Menten kinetics Brevibacillus borstelensis
2.4.2.2 additional information
-
 additional information Michaelis-Menten kinetics Aneurinibacillus migulanus
2.4.2.2 additional information
-
 additional information Michaelis-Menten kinetics Lacticaseibacillus paracasei
2.4.2.2 additional information
-
 additional information Michaelis-Menten kinetics Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.2 thymidine + phosphate Brevibacillus borstelensis
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate Brevibacillus borstelensis LK01
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate Brevibacillus borstelensis
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate Brevibacillus borstelensis LK01
-
 uracil + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.2 Aneurinibacillus migulanus A0A0D1XZI7
-
-
2.4.2.2 Aneurinibacillus migulanus U603 A0A0D1XZI7
-
-
2.4.2.2 Brevibacillus borstelensis A0A1S5VH74
-
-
2.4.2.2 Brevibacillus borstelensis LK01 A0A1S5VH74
-
-
2.4.2.2 Lacticaseibacillus paracasei A0A0E2M090
-
-
2.4.2.2 Serratia marcescens A0A080UUN2
-
-
2.4.2.2 Serratia marcescens MH6 A0A080UUN2
-
-
2.4.2.2 Siminovitchia fordii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.2 28.4
-
 purified recombinant enzyme, pH 8.0, 50°C, substrate uridine Aneurinibacillus migulanus
2.4.2.2 37.5
-
 purified recombinant enzyme, pH 8.0, 50°C, substrate uridine Lacticaseibacillus paracasei
2.4.2.2 43.3
-
 purified recombinant enzyme, pH 8.5, 50°C, substrate uridine Serratia marcescens
2.4.2.2 55.8
-
 purified recombinant enzyme, pH 8.0, 50°C, substrate uridine Siminovitchia fordii
2.4.2.2 98.3
-
 purified recombinnat enzyme, pH 8.5, 50°C, substrate uridine Brevibacillus borstelensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.2 2'-deoxyuridine + phosphate
-
 Brevibacillus borstelensis uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-fluoro-2'-deoxyuridine + phosphate
-
 Brevibacillus borstelensis 5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-fluoro-2'-deoxyuridine + phosphate
-
 Brevibacillus borstelensis LK01 5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-methyluridine + phosphate
-
 Brevibacillus borstelensis 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-methyluridine + phosphate
-
 Brevibacillus borstelensis LK01 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 additional information under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine Brevibacillus borstelensis ?
-
-
2.4.2.2 additional information under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reaches the yield of 61-84%. No activity with arauridine, 2',3'-deoxyuridine, cytidine, or deoxycytidine Brevibacillus borstelensis LK01 ?
-
-
2.4.2.2 thymidine + phosphate
-
 Brevibacillus borstelensis thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate
-
 Brevibacillus borstelensis LK01 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Siminovitchia fordii uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Brevibacillus borstelensis uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Aneurinibacillus migulanus uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Lacticaseibacillus paracasei uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Serratia marcescens uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Serratia marcescens MH6 uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Brevibacillus borstelensis LK01 uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Aneurinibacillus migulanus U603 uracil + alpha-D-ribose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.2 AmPyNP
-
 Aneurinibacillus migulanus
2.4.2.2 AmPyNP
-
 Serratia marcescens
2.4.2.2 BbPyNP
-
 Brevibacillus borstelensis
2.4.2.2 BfPyNP
-
 Siminovitchia fordii
2.4.2.2 deoA
-
 Aneurinibacillus migulanus
2.4.2.2 deoA
-
 Lacticaseibacillus paracasei
2.4.2.2 deoA
-
 Serratia marcescens
2.4.2.2 LpPyNP
-
 Lacticaseibacillus paracasei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.2 50
-
 assay at Siminovitchia fordii
2.4.2.2 50
-
 assay at Brevibacillus borstelensis
2.4.2.2 50
-
 assay at Aneurinibacillus migulanus
2.4.2.2 50
-
 assay at Lacticaseibacillus paracasei
2.4.2.2 50
-
 assay at Serratia marcescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.2 40 70 activity range, profile overview, recombinant enzyme Brevibacillus borstelensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.2 40
-
 purified recombinant enzyme, pH 8.5, 1 h, 90% activity remaining Brevibacillus borstelensis
2.4.2.2 45
-
 purified recombinant enzyme, pH 8.5, 1 h, 80% activity remaining Brevibacillus borstelensis
2.4.2.2 50
-
 purified recombinant enzyme, pH 8.5, 1 h, 43.7% activity remaining Lacticaseibacillus paracasei
2.4.2.2 50
-
 purified recombinant enzyme, pH 8.5, 1 h, 49.6% activity remaining Siminovitchia fordii
2.4.2.2 50
-
 purified recombinant enzyme, pH 8.5, 1 h, 52.1% activity remaining Serratia marcescens
2.4.2.2 50
-
 purified recombinant enzyme, pH 8.5, 1 h, 65.6% activity remaining Aneurinibacillus migulanus
2.4.2.2 50
-
 purified recombinant enzyme, pH 8.5, 1 h, 75% activity remaining Brevibacillus borstelensis
2.4.2.2 55
-
 purified recombinant enzyme, pH 8.5, 1 h, 70% activity remaining Brevibacillus borstelensis
2.4.2.2 60
-
 purified recombinant enzyme, pH 8.5, 1 h, 10% activity remaining Brevibacillus borstelensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.2 8.5
-
-
 Brevibacillus borstelensis
2.4.2.2 8.5
-
 assay at Siminovitchia fordii
2.4.2.2 8.5
-
 assay at Aneurinibacillus migulanus
2.4.2.2 8.5
-
 assay at Lacticaseibacillus paracasei
2.4.2.2 8.5
-
 assay at Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.2 5.5 10.5 activity range, profile overview, recombinant enzyme Brevibacillus borstelensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.2 7 9.8 purified enzyme, stable at Brevibacillus borstelensis