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Literature summary extracted from

  • Agu, C.; Ujor, V.; Ezeji, T.
    Metabolic engineering of Clostridium beijerinckii to improve glycerol metabolism and furfural tolerance (2019), Biotechnol. Biofuels, 12, 50 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.29 gene dhaK, recombinant expression in Clostridium beijerinckii, coexpression with gene gldA1, encoding a glycerol dehydrogenase (Gldh) from Clostridium pasteurianum Clostridium pasteurianum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.29 additional information constitutive co-overexpression of gene gldA1 or dhaD1, encoding a glycerol dehydrogenase (Gldh), and gene dhaK, encoding dihydroxyacetone kinase, as a fused protein results in a significant payoff in cell growth and acetone-butanol-ethanol (ABE) production compared to expression of one Gldh. Overexpression of [(dhaD1 + gldA1) dhaK] improves butanol and ABE production by 70% and 50%, respectively, in the presence of 5 and 6 g/l furfural relative to the plasmid control. Constitutive overexpression of two Gldh [dhaD1 + gldA1] as a fused protein increases glycerol utilization by 43% relative to the plasmid control, thus, representing about 14% increase in glycerol consumption relative to overexpression of GldA1 or DhaD1 alone. With [(dhaD1 + gldA1) dhaK], 28.6% increase in glycerol utilization is observed (compared to 43% by dhaD1 + gldA1), relative to the plasmid control. Inability of recombinant Clostridium beijerinckii to metabolize glycerol as a sole substrate. Method development and evaluation, overview Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.29 ATP + glycerone Clostridium pasteurianum
-
 ADP + glycerone phosphate
-
 ?
2.7.1.29 ATP + glycerone Clostridium pasteurianum ATCC 6013
-
 ADP + glycerone phosphate
-
 ?
2.7.1.29 ATP + glycerone Clostridium pasteurianum DSM 525
-
 ADP + glycerone phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.29 Clostridium pasteurianum A0A0H3J0E7
-
-
2.7.1.29 Clostridium pasteurianum ATCC 6013 A0A0H3J0E7
-
-
2.7.1.29 Clostridium pasteurianum DSM 525 A0A0H3J0E7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.29 ATP + glycerone
-
 Clostridium pasteurianum ADP + glycerone phosphate
-
 ?
2.7.1.29 ATP + glycerone
-
 Clostridium pasteurianum ATCC 6013 ADP + glycerone phosphate
-
 ?
2.7.1.29 ATP + glycerone
-
 Clostridium pasteurianum DSM 525 ADP + glycerone phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.29 DhaK
-
 Clostridium pasteurianum