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Literature summary extracted from

  • Liu, W.; Zhang, B.; Jiang, R.
    Improving acetyl-CoA biosynthesis in Saccharomyces cerevisiae via the overexpression of pantothenate kinase and PDH bypass (2017), Biotechnol. Biofuels, 10, 41 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 gene CAB1, recombinant overexpression of PanK under a strong constitutive promoter (truncated HXT7 promoter) in Saccharomyces cerevisiae strain CENF09 resulting in strain CENFP01 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.33 additional information improvement of acetyl-CoA biosynthesis in Saccharomyces cerevisiae via the overexpression of pantothenate kinase and PDH bypass. PanK overexpression or PDH bypass introduction alone only lead to a 2.0fold or 6.74fold increase in naringenin titer, but the combination of both (strain CENFPAA01) results in 24.4fold increase as compared to the control (strain CENF09) in the presence of 0.5 mM substrate p-coumaric acid. The supplement of PanK substrate pantothenate results in another 19% increase in naringenin production Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.33 cytosol
-
 Saccharomyces cerevisiae 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.33 Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Saccharomyces cerevisiae
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate Saccharomyces cerevisiae BY4742
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate Saccharomyces cerevisiae ATCC 204508
-
 ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Saccharomyces cerevisiae Q04430
-
-
2.7.1.33 Saccharomyces cerevisiae ATCC 204508 Q04430
-
-
2.7.1.33 Saccharomyces cerevisiae BY4742 Q04430
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Saccharomyces cerevisiae ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Saccharomyces cerevisiae BY4742 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Saccharomyces cerevisiae ATCC 204508 ADP + (R)-4'-phosphopantothenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 Cab1
-
 Saccharomyces cerevisiae
2.7.1.33 PanK
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.7.1.33 metabolism PanK is the rate-limiting enzyme for CoA synthesis in Saccharomyces cerevisiae, acetyl-CoA metabolism and associated naringenin synthesis pathway overview Saccharomyces cerevisiae
2.7.1.33 physiological function pantothenate, the substrate for PanK and precursor for CoA, is directly related with acetyl-CoA biosynthesis in Saccharomyces cerevisiae. PanK is the rate-limiting step for CoA synthesis and pantothenate supplement helps to increase the CoA/acetyl-CoA level in mammalian and Escherichia coli cells, as well as in Saccharomyces cerevisiae cells Saccharomyces cerevisiae