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Literature summary extracted from

  • Sterritt, O.; Lang, E.; Kessans, S.; Ryan, T.; Demeler, B.; Jameson, G.; Parker, E.
    Structural and functional characterisation of the entry point to pyocyanin biosynthesis in Pseudomonas aeruginosa defines a new 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase subclass (2018), Biosci. Rep., 38, BSR20181605 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.54 structure of type II DAH7PS, encoded by phzC as part of the duplicated phenazine biosynthetic cluster, to 2.7 A resolution, space group C2221 with two DAH7PS chains present in the asymmetric unit Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.016
-
 D-erythrose 4-phosphate pH 7.5, 37°C, presence of Co2+ Pseudomonas aeruginosa
2.5.1.54 0.017
-
 phosphoenolpyruvate pH 7.5, 37°C, presence of Co2+ Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Pseudomonas aeruginosa G3XCJ9
-
-
2.5.1.54 Pseudomonas aeruginosa DSM 22644 G3XCJ9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Pseudomonas aeruginosa 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Pseudomonas aeruginosa DSM 22644 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 DAH7PS
-
 Pseudomonas aeruginosa
2.5.1.54 phzC
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 45
-
-
 Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.54 35 50
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 19.8
-
 D-erythrose 4-phosphate pH 7.5, 37°C, presence of Co2+ Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7.5
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 6.5 7.5
-
 Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.54 1160
-
 D-erythrose 4-phosphate pH 7.5, 37°C, presence of Co2+ Pseudomonas aeruginosa
2.5.1.54 1240
-
 phosphoenolpyruvate pH 7.5, 37°C, presence of Co2+ Pseudomonas aeruginosa