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Literature summary extracted from

  • Takahashi, H.; Aihara, Y.; Ogawa, Y.; Murata, Y.; Nakajima, K.I.; Iida, M.; Shirai, M.; Fujisaki, S.
    Suppression of phenotype of Escherichia coli mutant defective in farnesyl diphosphate synthase by overexpression of gene for octaprenyl diphosphate synthase (2018), Biosci. Biotechnol. Biochem., 82, 1003-1010 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10 hexahistidine-tagged octaprenyl diphosphate synthase Escherichia coli
2.5.1.90 hexahistidine-tagged octaprenyl diphosphate synthase Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.90 0.0007
-
 dimethylallyl diphosphate at pH 7.5 and 30°C Escherichia coli
2.5.1.90 0.34
-
 isopentenyl diphosphate with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.90 Mg2+ 1 mM used in assay conditions Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate Escherichia coli
-
 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate Escherichia coli K12
-
 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Escherichia coli P22939 K12 strain SF5
-
2.5.1.10 Escherichia coli K12 P22939 K12 strain SF5
-
2.5.1.90 Escherichia coli P0AD57
-
-
2.5.1.90 Escherichia coli P0AD57 K12 strain SF5
-
2.5.1.90 Escherichia coli K12 P0AD57 K12 strain SF5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 hexahistidine-tagged octaprenyl diphosphate synthase Escherichia coli
2.5.1.90 hexahistidine-tagged octaprenyl diphosphate synthase Escherichia coli
2.5.1.90 Ni-NTA agarose column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.90 430
-
 with dimethylallyl diphosphate as substrate, at pH 7.5 and 30°C Escherichia coli
2.5.1.90 3100
-
 with geranyl diphosphate as substrate, at pH 7.5 and 30°C Escherichia coli
2.5.1.90 31000
-
 with geranylgeranyl diphosphate as substrate, at pH 7.5 and 30°C Escherichia coli
2.5.1.90 58000
-
 with (2E,6E)-farnesyl diphosphate as substrate, at pH 7.5 and 30°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.10 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli K12 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate (2E,6E)-farnesyl diphosphate is the most effective substrate Escherichia coli 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Escherichia coli K12 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate (2E,6E)-farnesyl diphosphate is the most effective substrate Escherichia coli K12 5 diphosphate + all-trans-octaprenyl diphosphate
-
 ?
2.5.1.90 dimethylallyl diphosphate + isopentenyl diphosphate 0.7% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate
-
 ?
2.5.1.90 dimethylallyl diphosphate + isopentenyl diphosphate 0.7% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli K12 diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate
-
 ?
2.5.1.90 geranyl diphosphate + isopentenyl diphosphate 5.3% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli ?
-
 ?
2.5.1.90 geranyl diphosphate + isopentenyl diphosphate 5.3% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli K12 ?
-
 ?
2.5.1.90 geranylgeranyl diphosphate + isopentenyl diphosphate 53.4% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli ?
-
 ?
2.5.1.90 geranylgeranyl diphosphate + isopentenyl diphosphate 53.4% activity compared to (2E,6E)-farnesyl diphosphate Escherichia coli K12 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.90 ? x * 37000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 IspA
-
 Escherichia coli
2.5.1.90 IspB
-
 Escherichia coli
2.5.1.90 octaprenyl diphosphate synthase
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.5.1.10 malfunction slow growth of an Escherichia coli ispA null mutant lacking farnesyl diphosphate synthase. The growth rates of ispA mutant-transformants harboring a medium-copy number plasmid that carries ispA is almost the same as that of the wild-type strain Escherichia coli