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Literature summary extracted from
- Evaluation of acceptor selectivity of Lactococcus lactis ssp. lactis trehalose 6-phosphate phosphorylase in the reverse phosphorolysis and synthesis of a new sugar phosphate (2017), Biosci. Biotechnol. Biochem., 81, 1512-1519 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.216 | expression in Escherichia coli | Lactococcus lactis ssp. lactis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.216 | beta-D-glucose 1-phosphate | - |
Lactococcus lactis ssp. lactis |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.216 | 0.413 | - |
beta-D-glucose 1-phosphate | pH 5.5, 30°C | Lactococcus lactis ssp. lactis | |
| 2.4.1.216 | 1.85 | - |
glucose 6-phosphate | pH 5.5, 30°C | Lactococcus lactis ssp. lactis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.216 | 88387 | - |
calculated from sequence | Lactococcus lactis ssp. lactis |
| 2.4.1.216 | 90000 | - |
SDS-PAGE | Lactococcus lactis ssp. lactis |
| 2.4.1.216 | 184000 | - |
blue native PAGE | Lactococcus lactis ssp. lactis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.216 | Lactococcus lactis ssp. lactis | - |
- |
- |
| 2.4.1.216 | Lactococcus lactis ssp. lactis JCM 5805 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.216 | - |
Lactococcus lactis ssp. lactis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.216 | 4°C, activity of is retained after incubation at pH 4.5-9.0 for 24 h | Lactococcus lactis ssp. lactis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.216 | alpha,alpha-trehalose 6-phosphate + phosphate | - |
Lactococcus lactis ssp. lactis | glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
r | |
| 2.4.1.216 | alpha,alpha-trehalose 6-phosphate + phosphate | - |
Lactococcus lactis ssp. lactis JCM 5805 | glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
r | |
| 2.4.1.216 | glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
Lactococcus lactis ssp. lactis | alpha,alpha-trehalose 6-phosphate + phosphate | - |
r | |
| 2.4.1.216 | glucose 6-phosphate + beta-D-glucose 1-phosphate | - |
Lactococcus lactis ssp. lactis JCM 5805 | alpha,alpha-trehalose 6-phosphate + phosphate | - |
r | |
| 2.4.1.216 | mannose 6-phosphate + beta-D-glucose 1-phosphate | mannose 6-phosphate is a poor substrate. Relative activity compared to glucose 6-phosphate is 0.05% | Lactococcus lactis ssp. lactis | alpha-D-Glc-(1->1)-alpha-D-Man6P + phosphate | - |
? | |
| 2.4.1.216 | mannose 6-phosphate + beta-D-glucose 1-phosphate | mannose 6-phosphate is a poor substrate. Relative activity compared to glucose 6-phosphate is 0.05% | Lactococcus lactis ssp. lactis JCM 5805 | alpha-D-Glc-(1->1)-alpha-D-Man6P + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.216 | homodimer | 2 * 90000, SDS-PAGE | Lactococcus lactis ssp. lactis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.216 | TrePP | - |
Lactococcus lactis ssp. lactis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.216 | 40 | - |
- |
Lactococcus lactis ssp. lactis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.216 | 25 | 50 | 25°C: about 45% of maximal activity, 50°C: about 80% of maximal activity | Lactococcus lactis ssp. lactis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.216 | 30 | - |
activity is retained after incubation at pH 5.0-8.0 at 30°C for 15 min | Lactococcus lactis ssp. lactis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.216 | 759 | - |
glucose 6-phosphate | pH 5.5, 30°C | Lactococcus lactis ssp. lactis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.216 | 4.8 | - |
synthesis of alpha,alpha-trehalose 6-phosphate | Lactococcus lactis ssp. lactis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.216 | 4.5 | 9 | activity is retained after incubation at pH 4.5-9.0 at 4°C for 24 h | Lactococcus lactis ssp. lactis |
| 2.4.1.216 | 5 | 8 | activity is retained after incubation at pH 5.0-8.0 at 30°C for 15 min | Lactococcus lactis ssp. lactis |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.216 | 0.519 | - |
beta-D-glucose 1-phosphate | pH 5.5, 30°C | Lactococcus lactis ssp. lactis | |
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