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Literature summary extracted from

  • Rinaldi, F.; Fernandez-Lucas, J.; de la Fuente, D.; Zheng, C.; Bavaro, T.; Peters, B.; Massolini, G.; Annunziata, F.; Conti, P.; de la Mata, I.; Terreni, M.; Calleri, E.
    Immobilized enzyme reactors based on nucleoside phosphorylases and 2'-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analogues (2020), Biores. Technol., 307, 123258 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.6 synthesis the enzyme can be used for in-flow synthesis of nucleoside analogues (2'-deoxy, 2',3'-dideoxy and arabinonucleoside derivatives) of pharmaceutical interest in mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs), method, overview Limosilactobacillus reuteri

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.6 gene ndt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Limosilactobacillus reuteri

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.6 additional information development of mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) for biosynthetic purposes, evaluation of performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest. Two biocatalytic routes based on nucleoside 2'-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and on uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) are investigated in the synthesis of 2'-deoxy, 2',3'-dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzes the synthesis of 5-fluoro-2'-deoxyuridine and 5-iodo-2'-deoxyuridine in 65-59% conversion yield, while CpUP/AhPNP-IMER provides the best results for the preparation of arabinosyladenine with 60% conversion yield. Enzyme LrNDT is immobilized on an epoxy silica monolithic resin by an in situ procedure (30% for LrNDT immobilization rate) Limosilactobacillus reuteri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Limosilactobacillus reuteri
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Limosilactobacillus reuteri SD2112
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2 Limosilactobacillus reuteri ATCC 55730
-
 2-deoxy-D-ribosyl-base2 + base1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.6 Limosilactobacillus reuteri F8DQT0
-
-
2.4.2.6 Limosilactobacillus reuteri ATCC 55730 F8DQT0
-
-
2.4.2.6 Limosilactobacillus reuteri SD2112 F8DQT0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and tag cleavage through thrombin Limosilactobacillus reuteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.6 2'-deoxyuridine + adenine immobilized recombinant enzyme, high activity Limosilactobacillus reuteri uracil + 2'-deoxyadenosine
-
 ?
2.4.2.6 2'-deoxyuridine + adenine immobilized recombinant enzyme, high activity Limosilactobacillus reuteri SD2112 uracil + 2'-deoxyadenosine
-
 ?
2.4.2.6 2'-deoxyuridine + adenine immobilized recombinant enzyme, high activity Limosilactobacillus reuteri ATCC 55730 uracil + 2'-deoxyadenosine
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Limosilactobacillus reuteri 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Limosilactobacillus reuteri SD2112 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 2-deoxy-D-ribosyl-base1 + base2
-
 Limosilactobacillus reuteri ATCC 55730 2-deoxy-D-ribosyl-base2 + base1
-
 ?
2.4.2.6 5-fluorouracil + 2'-deoxyadenosine immobilized recombinant enzyme Limosilactobacillus reuteri 5-fluoro-2'-deoxyuridine + adenine
-
 ?
2.4.2.6 5-fluorouracil + 2'-deoxyadenosine immobilized recombinant enzyme Limosilactobacillus reuteri SD2112 5-fluoro-2'-deoxyuridine + adenine
-
 ?
2.4.2.6 5-fluorouracil + 2'-deoxyadenosine immobilized recombinant enzyme Limosilactobacillus reuteri ATCC 55730 5-fluoro-2'-deoxyuridine + adenine
-
 ?
2.4.2.6 5-fluorouracil + 2'-deoxyuridine immobilized recombinant enzyme Limosilactobacillus reuteri 5-fluoro-2'-deoxyuridine + uracil
-
 ?
2.4.2.6 5-fluorouracil + 2'-deoxyuridine immobilized recombinant enzyme Limosilactobacillus reuteri SD2112 5-fluoro-2'-deoxyuridine + uracil
-
 ?
2.4.2.6 5-fluorouracil + 2'-deoxyuridine immobilized recombinant enzyme Limosilactobacillus reuteri ATCC 55730 5-fluoro-2'-deoxyuridine + uracil
-
 ?
2.4.2.6 5-iodouracil + 2'-deoxyadenosine immobilized recombinant enzyme Limosilactobacillus reuteri 5-iodo-2'-deoxyuridine + adenine
-
 ?
2.4.2.6 5-iodouracil + 2'-deoxyuridine immobilized recombinant enzyme Limosilactobacillus reuteri 5-iodo-2'-deoxyuridine + uracil
-
 ?
2.4.2.6 hypoxanthine + 2',3'-dideoxyuridine
-
 Limosilactobacillus reuteri 2',3'-dideoxyinosine + uracil
-
 ?
2.4.2.6 additional information arabinosyluracil is a poor substrate for the immobilized recombinant enzyme Limosilactobacillus reuteri ?
-
-
2.4.2.6 additional information arabinosyluracil is a poor substrate for the immobilized recombinant enzyme Limosilactobacillus reuteri SD2112 ?
-
-
2.4.2.6 additional information arabinosyluracil is a poor substrate for the immobilized recombinant enzyme Limosilactobacillus reuteri ATCC 55730 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.4.2.6 LrNDT
-
 Limosilactobacillus reuteri
2.4.2.6 NDT
-
 Limosilactobacillus reuteri
2.4.2.6 nucleoside 2'-deoxyribosyltransferase
-
 Limosilactobacillus reuteri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.6 37 40 assay at Limosilactobacillus reuteri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.6 6.5 7 assay at Limosilactobacillus reuteri