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Literature summary extracted from

  • Jiao, W.; Mittelstaedt, G.; Moggre, G.J.; Parker, E.J.
    Hinge twists and population shifts deliver regulated catalysis for ATP-PRT in histidine biosynthesis (2019), Biophys. J., 116, 1887-1897 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.17 expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.17 R216A the mutant shows more than 50% inhibition at 0.25 mM AMP and complete loss of response to histidine while retaining its histidine-binding ability Campylobacter jejuni
2.4.2.17 R216Q the mutant shows about 50% inhibition at 0.25 mM AMP, about 20% inhibition at 0.025 mM L-histidine, and about 45% inhibition at 0.25 mM ATP plus 0.025 mM L-histidine Campylobacter jejuni

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 AMP about 10% inhibition at 0.25 mM Campylobacter jejuni
2.4.2.17 L-histidine complete inhibition at 1 mM, about 10% inhibition at 0.25 mM Campylobacter jejuni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.17 Mg2+ dependent on, 10 mM used in assay conditions Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Campylobacter jejuni
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Campylobacter jejuni RM1221
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Campylobacter jejuni
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Campylobacter jejuni RM1221
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Campylobacter jejuni Q5HSJ4
-
-
2.4.2.17 Campylobacter jejuni RM1221 Q5HSJ4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.17
-
 Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Campylobacter jejuni ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Campylobacter jejuni RM1221 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Campylobacter jejuni 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Campylobacter jejuni RM1221 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.2.17 ATP-phosphoribosyltransferase
-
 Campylobacter jejuni
2.4.2.17 ATP-PRT
-
 Campylobacter jejuni