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Literature summary extracted from

  • Johannsson, S.; Neumann, P.; Ficner, R.
    Crystal structure of the human tRNA guanine transglycosylase catalytic subunit QTRT1 (2018), Biomolecules, 8, 81 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.29 expressed in Escherichia coli BL21(DE3)-STAR cells Homo sapiens
2.4.2.64 expression in Escherichia coli BL21(DE3) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.29 apoenzyme and in complex with queuine, sitting or hanging drop vapor diffusion method, using 100 mM Tris pH 7.8, 200 mM KBr, 200 mM KSCN, 3% (w/v) polyglutamic acid-LM, 5% (w/v) PEG 4000 Homo sapiens
2.4.2.64 sitting-drop or hanging drop vapor diffusion technique, crystal structure of the human tRNA guanine transglycosylase catalytic subunit QTRT1 in its apo form and in complex with the queuine base Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.29 guanine34 in tRNA + queuine Homo sapiens
-
 queuine34 in tRNA + guanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.29 Homo sapiens Q9BXR0
-
-
2.4.2.64 Homo sapiens Q9BXR0 AND Q9H974 Q9BXR0: catalytic subunit 1 (QTRT1), Q9H974: accessory subunit 2 (QTRT2)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.29 His-Talon column chromatography and Superdex S200 gel filtration Homo sapiens
2.4.2.64
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.29 guanine34 in tRNA + queuine
-
 Homo sapiens queuine34 in tRNA + guanine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.29 heterodimer
-
 Homo sapiens
2.4.2.64 heterodimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.2.29 QTRT1 catalytic subunit Homo sapiens
2.4.2.29 queuine tRNA-ribosyltransferase subunit 1
-
 Homo sapiens
2.4.2.29 TGT
-
 Homo sapiens
2.4.2.29 tRNA guanine transglycosylase
-
 Homo sapiens
2.4.2.64 QTRT1 gene name, catalytic subunit 1 Homo sapiens
2.4.2.64 QTRT2 gene name, accessory subunit 2 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.64 7.8
-
-
 Homo sapiens