Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tu, C.; Zhou, J.; Peng, L.; Man, S.; Ma, L.
    Self-assembled nano-aggregates of fluorinases demonstrate enhanced enzymatic activity, thermostability and reusability (2020), Biomater. Sci., 8, 648-656 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.63 expression in Escherichia coli Streptomyces xinghaiensis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.63 additional information fusion of self-assembling amphipathic peptides into the C-terminus of fluorinase leads to proteins capable of self-assembly to form nano-sized particles with different dimensions in aqueous solutions. Peptide LLLLLLKD-tagged enzyme displays improved enzyme activity, thermostability and reusability Streptomyces xinghaiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.63 0.0351
-
 S-adenosyl-L-methionine (LELELKLK)2-tagged enzyme, pH 6.0, 40°C Streptomyces xinghaiensis
2.5.1.63 0.0362
-
 S-adenosyl-L-methionine wild-type, pH 6.0, 40°C Streptomyces xinghaiensis
2.5.1.63 0.0411
-
 S-adenosyl-L-methionine LLLLLLKD-tagged enzyme, pH 6.0, 50°C Streptomyces xinghaiensis
2.5.1.63 0.0721
-
 S-adenosyl-L-methionine EWLKAFYEKVLEKLKELF-tagged enzyme, pH 6.0, 60°C Streptomyces xinghaiensis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.63 Streptomyces xinghaiensis A0A068VNW5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.63 S-adenosyl-L-methionine + fluoride
-
 Streptomyces xinghaiensis 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.63 FlA4
-
 Streptomyces xinghaiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.63 40
-
 wild-type Streptomyces xinghaiensis
2.5.1.63 50
-
 peptide LLLLLLKD-tagged enzyme Streptomyces xinghaiensis
2.5.1.63 60
-
 peptide EWLKAFYEKVLEKLKELF-tagged enzyme Streptomyces xinghaiensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.63 40
-
 half-life of wild-type, (LELELKLK)2-tagged enzyme is 52 and 55.9 min, respectively Streptomyces xinghaiensis
2.5.1.63 50
-
 half-life of wild-type, (LELELKLK)2-tagged enzyme, LLLLLLKD-tagged enzyme is 1.12, 3.28 and 82.5 min, respectively Streptomyces xinghaiensis
2.5.1.63 60
-
 half-life of LLLLLLKD-tagged enzyme and EWLKAFYEKVLEKLKELF-tagged enzyme is 0.72 and 2.49 min, respectively Streptomyces xinghaiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.63 0.0022
-
 S-adenosyl-L-methionine (LELELKLK)2-tagged enzyme, pH 6.0, 40°C Streptomyces xinghaiensis
2.5.1.63 0.0026
-
 S-adenosyl-L-methionine wild-type, pH 6.0, 40°C Streptomyces xinghaiensis
2.5.1.63 0.0034
-
 S-adenosyl-L-methionine LLLLLLKD-tagged enzyme, pH 6.0, 50°C Streptomyces xinghaiensis
2.5.1.63 0.0047
-
 S-adenosyl-L-methionine EWLKAFYEKVLEKLKELF-tagged enzyme, pH 6.0, 60°C Streptomyces xinghaiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.63 6
-
-
 Streptomyces xinghaiensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.63 0.062
-
 S-adenosyl-L-methionine (LELELKLK)2-tagged enzyme, pH 6.0, 40°C Streptomyces xinghaiensis
2.5.1.63 0.065
-
 S-adenosyl-L-methionine EWLKAFYEKVLEKLKELF-tagged enzyme, pH 6.0, 60°C Streptomyces xinghaiensis
2.5.1.63 0.076
-
 S-adenosyl-L-methionine wild-type, pH 6.0, 40°C Streptomyces xinghaiensis
2.5.1.63 0.082
-
 S-adenosyl-L-methionine LLLLLLKD-tagged enzyme, pH 6.0, 50°C Streptomyces xinghaiensis