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Literature summary extracted from

  • Singh, K.; Singh, K.P.; Equbal, A.; Suman, S.S.; Zaidi, A.; Garg, G.; Pandey, K.; Das, P.; Ali, V.
    Interaction between cysteine synthase and serine O-acetyltransferase proteins and their stage specific expression in Leishmania donovani (2016), Biochimie, 131, 29-44 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 expression in Escherichia coli Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.17
-
 H2S pH 7.5, 37°C Leishmania donovani
2.5.1.47 15.86
-
 O-acetyl-L-Ser pH 7.5, 37°C Leishmania donovani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.47 cytoplasm
-
 Leishmania donovani 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 72000
-
 gel filtration Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Leishmania donovani G1C2I2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 recombinant protein Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 promastigote
-
 Leishmania donovani
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.47 -80°C, 10% glycerol, stable for more than 3 months Leishmania donovani
2.5.1.47 4°C, 10-15% glycerol, 24 h, no loss of activity Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Leishmania donovani L-Cys + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer x * 34500, calculated from sequence, x * 38000, SDS-PAGE of His-tagged recombinant protein Leishmania donovani
2.5.1.47 More serine O-acetyltransferase and cysteine synthase form stable complexes containing two serine O-acetyltransferase trimers and two cysteine synthase dimers Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.5
-
-
 Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate at about 1:1 stoichiometric ratio Leishmania donovani

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.47 Leishmania donovani calculated from sequence
-
 8.2

Expression

EC Number Organism Comment Expression
2.5.1.47 Leishmania donovani serine O-acetyltransferase and cysteine synthase proteins expression/activity is upregulated in amastigote growth stage up