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Literature summary extracted from

  • McAuley, M.; Huang, M.; Timson, D.J.
    Insight into the mechanism of galactokinase Role of a critical glutamate residue and helix/coil transitions (2017), Biochim. Biophys. Acta Proteins Proteom., 1865, 321-328 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.6 expression in Escherichia coli HMS174(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.6 E174Q no detectable activity, less stable enzyme Homo sapiens
2.7.1.6 R105M modest reduction in activity with little change in stability Homo sapiens
2.7.1.6 R228M interaction with both ATP and galactose is affected. The variant is significantly less stable than the wild-type protein Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.6 0.0039
-
 ATP mutant enzyme E174D, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.004
-
 ATP wild-type enzyme, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.0094
-
 ATP mutant enzyme R228M, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.0149
-
 ATP mutant enzyme R228K, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.083
-
 ATP mutant enzyme R105M, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.22
-
 alpha-D-galactose mutant enzyme E174D, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.26
-
 alpha-D-galactose mutant enzyme R105M, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.31
-
 alpha-D-galactose mutant enzyme R228K, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.33
-
 alpha-D-galactose wild-type enzyme, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.67
-
 alpha-D-galactose mutant enzyme R228M, pH 7.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.6 ATP + alpha-D-galactose Homo sapiens the enzyme catalyses the first committed step in the Leloir pathway ADP + alpha-D-galactose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.6 Homo sapiens P51570
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.6
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.6 ATP + alpha-D-galactose the enzyme catalyses the first committed step in the Leloir pathway Homo sapiens ADP + alpha-D-galactose 1-phosphate
-
 ?
2.7.1.6 ATP + alpha-D-galactose negative charge at residue 174 is essential for catalysis Homo sapiens ADP + alpha-D-galactose 1-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.6 51.3
-
 Tm-value, mutant enzyme R228K Homo sapiens
2.7.1.6 51.68
-
 Tm-value, mutant enzyme R228M Homo sapiens
2.7.1.6 52.5
-
 Tm-value, mutant enzyme E174Q Homo sapiens
2.7.1.6 55.13
-
 Tm-value, mutant enzyme E174D Homo sapiens
2.7.1.6 55.42
-
 Tm-value, mutant enzyme R105M Homo sapiens
2.7.1.6 55.72
-
 Tm-value, wild-type enzyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.6 0.7
-
 ATP mutant enzyme R105M, pH 7.5, 37°C Homo sapiens
2.7.1.6 0.7
-
 alpha-D-galactose mutant enzyme R105M, pH 7.5, 37°C Homo sapiens
2.7.1.6 1.6
-
 ATP mutant enzyme E174D, pH 7.5, 37°C Homo sapiens
2.7.1.6 1.6
-
 alpha-D-galactose mutant enzyme E174D, pH 7.5, 37°C Homo sapiens
2.7.1.6 1.7
-
 ATP wild-type enzyme, pH 7.5, 37°C Homo sapiens
2.7.1.6 1.7
-
 alpha-D-galactose wild-type enzyme, pH 7.5, 37°C Homo sapiens
2.7.1.6 2.3
-
 ATP mutant enzyme R228M, pH 7.5, 37°C Homo sapiens
2.7.1.6 2.3
-
 alpha-D-galactose mutant enzyme R228M, pH 7.5, 37°C Homo sapiens
2.7.1.6 3.7
-
 ATP mutant enzyme R228K, pH 7.5, 37°C Homo sapiens
2.7.1.6 3.7
-
 alpha-D-galactose mutant enzyme R228K, pH 7.5, 37°C Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.6 drug target possible drug target in the treatment of type I galactosemia Homo sapiens
2.7.1.6 metabolism the enzyme catalyses the first committed step in the Leloir pathway Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.6 2.7
-
 alpha-D-galactose mutant enzyme R105M, pH 7.5, 37°C Homo sapiens
2.7.1.6 3.3
-
 alpha-D-galactose mutant enzyme R228M, pH 7.5, 37°C Homo sapiens
2.7.1.6 7.6
-
 alpha-D-galactose mutant enzyme E174D, pH 7.5, 37°C Homo sapiens
2.7.1.6 10.1
-
 alpha-D-galactose wild-type enzyme, pH 7.5, 37°C Homo sapiens
2.7.1.6 19.6
-
 alpha-D-galactose mutant enzyme R228K, pH 7.5, 37°C Homo sapiens
2.7.1.6 98
-
 ATP mutant enzyme R105M, pH 7.5, 37°C Homo sapiens
2.7.1.6 240
-
 ATP mutant enzyme R228M, pH 7.5, 37°C Homo sapiens
2.7.1.6 250
-
 ATP mutant enzyme R228K, pH 7.5, 37°C Homo sapiens
2.7.1.6 340
-
 ATP mutant enzyme E174D, pH 7.5, 37°C Homo sapiens
2.7.1.6 430
-
 ATP wild-type enzyme, pH 7.5, 37°C Homo sapiens