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Literature summary extracted from

  • Kamel, S.; Thiele, I.; Neubauer, P.; Wagner, A.
    Thermophilic nucleoside phosphorylases their properties, characteristics and applications (2020), Biochim. Biophys. Acta, 1868, 140304 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.1 synthesis enzyme is useful in biocatalytic production of 2,6-diaminopurine 2'-deoxyriboside Thermus thermophilus
2.4.2.1 synthesis enzyme is useful in biocatalytic production of 2,6-diaminopurine riboside and 5-methyluridine Aeropyrum pernix
2.4.2.1 synthesis enzyme is useful in biocatalytic production of 2,6-diaminopurine riboside, 2,6-dichloropurine riboside, 6-chloro-2-fluoropurine riboside, 2-chloroadenine riboside, 2-fluoroadenine riboside, 2,6-diaminopurine 2'-deoxyriboside, 2,6-dichloropurine 2'-deoxyriboside, 6-chloro-2-fluoropurine 2'-deoxyriboside, 2-chloro-2'-deoxyadenosine (cladribine), and 2-fluoro-2'-deoxyadenosine, as well as of 2,6-diaminopurine arabinoside, 2,6-dichloropurine arabinoside, and 6-chloro-2-fluoropurine arabinoside Parageobacillus thermoglucosidasius
2.4.2.1 synthesis enzyme is useful in biocatalytic production of 2-chloro-2'-deoxyadenosine (Cladribine), and 2-fluoroadenine arabinoside Geobacillus stearothermophilus
2.4.2.1 synthesis enzyme is useful in biocatalytic production of 2-fluoro-2'-deoxyadenosine and 2'-deoxy-2'-fluoroadenosine Deinococcus geothermalis
2.4.2.1 synthesis enzyme is useful in biocatalytic production of 5-methyluridine Geobacillus stearothermophilus
2.4.2.2 synthesis the enzyme is useful in biocatalytic production of 2,6-dichloropurine 2'-deoxyriboside and 6-chloro-2-fluoropurine 2'-deoxyriboside Parageobacillus thermoglucosidasius
2.4.2.2 synthesis the enzyme is useful in biocatalytic production of 2-chloro-2'-deoxyadenosine (cladribine), adenine-arabinoside, 2-fluoroadenine arabinoside, and 5-methyluridine Geobacillus stearothermophilus
2.4.2.2 synthesis the enzyme is useful in biocatalytic production of 2-chloroadenine riboside, 2-fluoroadenine riboside, 2,6-diaminopurine 2'-deoxyriboside, 2-chloro-2'-deoxyadenosine, 2-fluoro-2'-deoxyadenosine, 2'-deoxy-2'-fluoroadenosine, 2'-deoxy-2'-fluoro-arabinofuranosyl adenine, 2,6-diaminopurine arabinoside, 2,6-dichloropurine arabinoside, 6-chloro-2-fluoropurine arabinoside, and 5-iododeoxyuridine Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.1 adenosine + phosphate Parageobacillus thermoglucosidasius
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Pyrococcus furiosus
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Deinococcus geothermalis
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Thermus thermophilus
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Aeropyrum pernix
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Geobacillus stearothermophilus
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Pyrococcus furiosus ATCC 43587
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Thermus thermophilus DSM 7039
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Pyrococcus furiosus Vc1
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Deinococcus geothermalis DSM 11300
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Deinococcus geothermalis AG-3a
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Parageobacillus thermoglucosidasius 11955
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Pyrococcus furiosus JCM 8422
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate Thermus thermophilus ATCC BAA-163
-
 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Parageobacillus thermoglucosidasius
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Pyrococcus furiosus
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Deinococcus geothermalis
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Thermus thermophilus
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Aeropyrum pernix
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Geobacillus stearothermophilus
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Pyrococcus furiosus ATCC 43587
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Thermus thermophilus DSM 7039
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Pyrococcus furiosus Vc1
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Deinococcus geothermalis DSM 11300
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Deinococcus geothermalis AG-3a
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Parageobacillus thermoglucosidasius 11955
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Pyrococcus furiosus JCM 8422
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate Thermus thermophilus ATCC BAA-163
-
 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate Thermus thermophilus
-
 uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate Geobacillus stearothermophilus
-
 uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate Parageobacillus thermoglucosidasius
-
 uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate Thermus thermophilus
-
 uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate Geobacillus stearothermophilus
-
 uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate Parageobacillus thermoglucosidasius
-
 uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
 r
2.4.2.2 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate Thermus thermophilus
-
 uracil + 2-deoxy-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate Geobacillus stearothermophilus
-
 uracil + 2-deoxy-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate Parageobacillus thermoglucosidasius
-
 uracil + 2-deoxy-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxythymidine + phosphate Thermus thermophilus
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 2'-deoxythymidine + phosphate Geobacillus stearothermophilus
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 2'-deoxythymidine + phosphate Parageobacillus thermoglucosidasius
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-thymidine + phosphate Thermus thermophilus
-
 5-bromo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-thymidine + phosphate Geobacillus stearothermophilus
-
 5-bromo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-thymidine + phosphate Parageobacillus thermoglucosidasius
-
 5-bromo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-uridine + phosphate Thermus thermophilus
-
 5-bromo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-uridine + phosphate Geobacillus stearothermophilus
-
 5-bromo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-uridine + phosphate Parageobacillus thermoglucosidasius
-
 5-bromo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-thymidine + phosphate Thermus thermophilus
-
 5-iodo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-thymidine + phosphate Geobacillus stearothermophilus
-
 5-iodo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-thymidine + phosphate Parageobacillus thermoglucosidasius
-
 5-iodo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-uridine + phosphate Thermus thermophilus
-
 5-iodo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-uridine + phosphate Geobacillus stearothermophilus
-
 5-iodo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-uridine + phosphate Parageobacillus thermoglucosidasius
-
 5-iodo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-methyluridine + phosphate Thermus thermophilus
-
 5-methyluracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-methyluridine + phosphate Geobacillus stearothermophilus
-
 5-methyluracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-methyluridine + phosphate Parageobacillus thermoglucosidasius
-
 5-methyluracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate Thermus thermophilus
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate Geobacillus stearothermophilus
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate Parageobacillus thermoglucosidasius
-
 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate Thermus thermophilus
-
 thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate Geobacillus stearothermophilus
-
 thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate Parageobacillus thermoglucosidasius
-
 thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 uridine + phosphate Thermus thermophilus
-
 uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 uridine + phosphate Geobacillus stearothermophilus
-
 uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 uridine + phosphate Parageobacillus thermoglucosidasius
-
 uracil + alpha-D-ribose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Aeropyrum pernix A0A401H9D3
-
-
2.4.2.1 Deinococcus geothermalis
-
-
-
2.4.2.1 Deinococcus geothermalis AG-3a
-
-
-
2.4.2.1 Deinococcus geothermalis DSM 11300
-
-
-
2.4.2.1 Geobacillus stearothermophilus P77834
-
-
2.4.2.1 Geobacillus stearothermophilus P77835
-
-
2.4.2.1 Parageobacillus thermoglucosidasius
-
 i.e. Parageobacillus thermoglucosidasius
-
2.4.2.1 Parageobacillus thermoglucosidasius 11955
-
 i.e. Parageobacillus thermoglucosidasius
-
2.4.2.1 Pyrococcus furiosus Q8U2I1
-
-
2.4.2.1 Pyrococcus furiosus ATCC 43587 Q8U2I1
-
-
2.4.2.1 Pyrococcus furiosus JCM 8422 Q8U2I1
-
-
2.4.2.1 Pyrococcus furiosus Vc1 Q8U2I1
-
-
2.4.2.1 Thermus thermophilus Q72IR2
-
-
2.4.2.1 Thermus thermophilus Q72L69
-
-
2.4.2.1 Thermus thermophilus ATCC BAA-163 Q72IR2
-
-
2.4.2.1 Thermus thermophilus ATCC BAA-163 Q72L69
-
-
2.4.2.1 Thermus thermophilus DSM 7039 Q72IR2
-
-
2.4.2.1 Thermus thermophilus DSM 7039 Q72L69
-
-
2.4.2.2 Bacillus subtilis P39142
-
-
2.4.2.2 Bacillus subtilis 168 P39142
-
-
2.4.2.2 Geobacillus stearothermophilus P77836
-
-
2.4.2.2 Parageobacillus thermoglucosidasius A0A178U4P5
-
-
2.4.2.2 Thermus thermophilus Q72HS4
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.4.2.1 the enzyme is stable at 5%-20% DMSO Parageobacillus thermoglucosidasius
2.4.2.1 the enzyme is stable at 5%-20% DMSO Deinococcus geothermalis
2.4.2.1 the enzyme is stable at 50% DMF, 50% DMSO, and 50% 2-MeTHF Thermus thermophilus
2.4.2.2 the purified enzyme is stable at 50% DMF, 50% DMSO, and 50% 2-MeTHF Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate
-
 Parageobacillus thermoglucosidasius adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Pyrococcus furiosus adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Deinococcus geothermalis adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Thermus thermophilus adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Aeropyrum pernix adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Geobacillus stearothermophilus adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Pyrococcus furiosus ATCC 43587 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Thermus thermophilus DSM 7039 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Pyrococcus furiosus Vc1 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Deinococcus geothermalis DSM 11300 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Deinococcus geothermalis AG-3a adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Parageobacillus thermoglucosidasius 11955 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Pyrococcus furiosus JCM 8422 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 adenosine + phosphate
-
 Thermus thermophilus ATCC BAA-163 adenine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Parageobacillus thermoglucosidasius hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Pyrococcus furiosus hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Deinococcus geothermalis hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Thermus thermophilus hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Aeropyrum pernix hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Geobacillus stearothermophilus hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Pyrococcus furiosus ATCC 43587 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Thermus thermophilus DSM 7039 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Pyrococcus furiosus Vc1 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Deinococcus geothermalis DSM 11300 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Deinococcus geothermalis AG-3a hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Parageobacillus thermoglucosidasius 11955 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Pyrococcus furiosus JCM 8422 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Thermus thermophilus ATCC BAA-163 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Parageobacillus thermoglucosidasius ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Pyrococcus furiosus ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Deinococcus geothermalis ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Thermus thermophilus ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Aeropyrum pernix ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Geobacillus stearothermophilus ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Pyrococcus furiosus ATCC 43587 ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Thermus thermophilus DSM 7039 ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Pyrococcus furiosus Vc1 ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Deinococcus geothermalis DSM 11300 ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Deinococcus geothermalis AG-3a ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Parageobacillus thermoglucosidasius 11955 ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Pyrococcus furiosus JCM 8422 ?
-
-
2.4.2.1 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Thermus thermophilus ATCC BAA-163 ?
-
-
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
-
 Thermus thermophilus uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
-
 Geobacillus stearothermophilus uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate
-
 Parageobacillus thermoglucosidasius uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
-
 Thermus thermophilus uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
-
 Geobacillus stearothermophilus uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
 r
2.4.2.2 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate
-
 Parageobacillus thermoglucosidasius uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose
-
 r
2.4.2.2 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
-
 Thermus thermophilus uracil + 2-deoxy-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
-
 Geobacillus stearothermophilus uracil + 2-deoxy-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate
-
 Parageobacillus thermoglucosidasius uracil + 2-deoxy-beta-D-arabinofuranose
-
 r
2.4.2.2 2'-deoxythymidine + phosphate
-
 Thermus thermophilus thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 2'-deoxythymidine + phosphate
-
 Geobacillus stearothermophilus thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 2'-deoxythymidine + phosphate
-
 Parageobacillus thermoglucosidasius thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 2,6-dichloropurine 2'-deoxyriboside + phosphate
-
 Parageobacillus thermoglucosidasius ?
-
 r
2.4.2.2 5-bromo-thymidine + phosphate
-
 Thermus thermophilus 5-bromo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-thymidine + phosphate
-
 Geobacillus stearothermophilus 5-bromo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-thymidine + phosphate
-
 Parageobacillus thermoglucosidasius 5-bromo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-uridine + phosphate
-
 Thermus thermophilus 5-bromo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-uridine + phosphate
-
 Geobacillus stearothermophilus 5-bromo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-bromo-uridine + phosphate
-
 Parageobacillus thermoglucosidasius 5-bromo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-thymidine + phosphate
-
 Thermus thermophilus 5-iodo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-thymidine + phosphate
-
 Geobacillus stearothermophilus 5-iodo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-thymidine + phosphate
-
 Parageobacillus thermoglucosidasius 5-iodo-thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-uridine + phosphate
-
 Thermus thermophilus 5-iodo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-uridine + phosphate
-
 Geobacillus stearothermophilus 5-iodo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-iodo-uridine + phosphate
-
 Parageobacillus thermoglucosidasius 5-iodo-uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-methyluridine + phosphate
-
 Thermus thermophilus 5-methyluracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-methyluridine + phosphate
-
 Geobacillus stearothermophilus 5-methyluracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 5-methyluridine + phosphate
-
 Parageobacillus thermoglucosidasius 5-methyluracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 6-chloro-2-fluoropurine 2'-deoxyriboside + phosphate
-
 Parageobacillus thermoglucosidasius ?
-
 r
2.4.2.2 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Thermus thermophilus ?
-
-
2.4.2.2 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Geobacillus stearothermophilus ?
-
-
2.4.2.2 additional information thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview Parageobacillus thermoglucosidasius ?
-
-
2.4.2.2 thymidine + phosphate
-
 Thermus thermophilus thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate
-
 Geobacillus stearothermophilus thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate
-
 Parageobacillus thermoglucosidasius thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate
-
 Thermus thermophilus thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate
-
 Geobacillus stearothermophilus thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 thymidine + phosphate
-
 Parageobacillus thermoglucosidasius thymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 uridine + phosphate
-
 Thermus thermophilus uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 uridine + phosphate
-
 Geobacillus stearothermophilus uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 uridine + phosphate
-
 Parageobacillus thermoglucosidasius uracil + alpha-D-ribose 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 apPNP
-
 Aeropyrum pernix
2.4.2.1 DeoD
-
 Geobacillus stearothermophilus
2.4.2.1 deoD_1 gene name, UniProt Parageobacillus thermoglucosidasius
2.4.2.1 DgPNP
-
 Deinococcus geothermalis
2.4.2.1 GsPNPI
-
 Geobacillus stearothermophilus
2.4.2.1 GsPNPII
-
 Geobacillus stearothermophilus
2.4.2.1 GtPNP
-
 Parageobacillus thermoglucosidasius
2.4.2.1 PNP
-
 Parageobacillus thermoglucosidasius
2.4.2.1 PNP
-
 Pyrococcus furiosus
2.4.2.1 PNP
-
 Deinococcus geothermalis
2.4.2.1 PNP
-
 Thermus thermophilus
2.4.2.1 PNP
-
 Aeropyrum pernix
2.4.2.1 PNP
-
 Geobacillus stearothermophilus
2.4.2.1 PNP 1
-
 Geobacillus stearothermophilus
2.4.2.1 PNP 2
-
 Geobacillus stearothermophilus
2.4.2.1 PNP I
-
 Geobacillus stearothermophilus
2.4.2.1 PNP II
-
 Geobacillus stearothermophilus
2.4.2.1 pNPI
-
 Thermus thermophilus
2.4.2.1 PNPII
-
 Thermus thermophilus
2.4.2.1 punA
-
 Geobacillus stearothermophilus
2.4.2.1 purine nucleoside phosphorylase 1
-
 Geobacillus stearothermophilus
2.4.2.1 purine nucleoside phosphorylase 2
-
 Parageobacillus thermoglucosidasius
2.4.2.1 purine nucleoside phosphorylase 2
-
 Geobacillus stearothermophilus
2.4.2.1 purine nucleoside phosphorylase DeoD-type UniProt Geobacillus stearothermophilus
2.4.2.1 TTC0194 locus name Thermus thermophilus
2.4.2.1 TTC1070 locus name Thermus thermophilus
2.4.2.1 TtPNPI
-
 Thermus thermophilus
2.4.2.1 TtPNPII
-
 Thermus thermophilus
2.4.2.1 ylmD
-
 Parageobacillus thermoglucosidasius
2.4.2.2 BsPyNP
-
 Bacillus subtilis
2.4.2.2 GsPyNP
-
 Geobacillus stearothermophilus
2.4.2.2 GtPyNP
-
 Parageobacillus thermoglucosidasius
2.4.2.2 PDP
-
 Bacillus subtilis
2.4.2.2 PYNP
-
 Bacillus subtilis
2.4.2.2 PYNP
-
 Thermus thermophilus
2.4.2.2 PYNP
-
 Geobacillus stearothermophilus
2.4.2.2 PYNP
-
 Parageobacillus thermoglucosidasius
2.4.2.2 pyrimidine nucleoside phosphorylase
-
 Bacillus subtilis
2.4.2.2 pyrimidine nucleoside phosphorylase
-
 Thermus thermophilus
2.4.2.2 pyrimidine nucleoside phosphorylase
-
 Geobacillus stearothermophilus
2.4.2.2 pyrimidine nucleoside phosphorylase
-
 Parageobacillus thermoglucosidasius
2.4.2.2 TtPyNP
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 55
-
-
 Parageobacillus thermoglucosidasius
2.4.2.1 70
-
-
 Deinococcus geothermalis
2.4.2.1 70
-
-
 Geobacillus stearothermophilus
2.4.2.1 80
-
-
 Geobacillus stearothermophilus
2.4.2.1 99
-
 above Thermus thermophilus
2.4.2.1 100
-
-
 Aeropyrum pernix
2.4.2.1 120
-
-
 Pyrococcus furiosus
2.4.2.2 60
-
-
 Parageobacillus thermoglucosidasius
2.4.2.2 70
-
-
 Geobacillus stearothermophilus
2.4.2.2 99
-
 above Bacillus subtilis
2.4.2.2 99
-
 above Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.1 additional information
-
 thermophilic signature of the enzyme, overview Parageobacillus thermoglucosidasius
2.4.2.1 additional information
-
 thermophilic signature of the enzyme, overview Pyrococcus furiosus
2.4.2.1 additional information
-
 thermophilic signature of the enzyme, overview Deinococcus geothermalis
2.4.2.1 additional information
-
 thermophilic signature of the enzyme, overview Thermus thermophilus
2.4.2.1 additional information
-
 thermophilic signature of the enzyme, overview Aeropyrum pernix
2.4.2.1 additional information
-
 thermophilic signature of the enzyme, overview Geobacillus stearothermophilus
2.4.2.2 additional information
-
 thermophilic signature of the enzyme, overview Bacillus subtilis
2.4.2.2 additional information
-
 thermophilic signature of the enzyme, overview Thermus thermophilus
2.4.2.2 additional information
-
 thermophilic signature of the enzyme, overview Geobacillus stearothermophilus
2.4.2.2 additional information
-
 thermophilic signature of the enzyme, overview Parageobacillus thermoglucosidasius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 55
-
 half-life is over 8 h Parageobacillus thermoglucosidasius
2.4.2.1 60
-
 half-life is 1.6 h Parageobacillus thermoglucosidasius
2.4.2.1 70
-
 half-life is over 3 h Geobacillus stearothermophilus
2.4.2.1 70
-
 half-life is over 8 h Deinococcus geothermalis
2.4.2.1 80
-
 half-life is 132 h Thermus thermophilus
2.4.2.1 80
-
 half-life is 15 min Geobacillus stearothermophilus
2.4.2.1 80
-
 half-life is 232 h Thermus thermophilus
2.4.2.1 100
-
 half-life is over 60 min Aeropyrum pernix
2.4.2.1 105
-
 half-life is 69 min Pyrococcus furiosus
2.4.2.1 110
-
 half-life is 12 min Pyrococcus furiosus
2.4.2.1 115
-
 half-life is 5 min Pyrococcus furiosus
2.4.2.2 60
-
 purified enzyme, half-life is over 16 h Parageobacillus thermoglucosidasius
2.4.2.2 70
-
 purified enzyme, half-life is 1.6 h Parageobacillus thermoglucosidasius
2.4.2.2 70
-
 purified enzyme, half-life is 23 min in the presence of 20 mM potassium phosphate and 1 mM inosine, and 15.1 h in the presence of 20 mM potassium phosphate and 1 mM 5-methyluridine Geobacillus stearothermophilus
2.4.2.2 80
-
 purified enzyme, half-life is over 23 h Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 5
-
-
 Thermus thermophilus
2.4.2.1 5
-
-
 Aeropyrum pernix
2.4.2.1 5 6
-
 Thermus thermophilus
2.4.2.1 7
-
-
 Parageobacillus thermoglucosidasius
2.4.2.1 7
-
-
 Pyrococcus furiosus
2.4.2.1 7
-
-
 Deinococcus geothermalis
2.4.2.1 7 11
-
 Geobacillus stearothermophilus
2.4.2.1 7.5 11
-
 Geobacillus stearothermophilus
2.4.2.2 5 6
-
 Bacillus subtilis
2.4.2.2 5 6
-
 Thermus thermophilus
2.4.2.2 7 11.5
-
 Geobacillus stearothermophilus
2.4.2.2 7.5 8.5
-
 Parageobacillus thermoglucosidasius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.1 3 8
-
 Thermus thermophilus
2.4.2.1 3 11
-
 Aeropyrum pernix

General Information

EC Number General Information Comment Organism
2.4.2.2 evolution the enzyme belongs to the NP family II Thermus thermophilus
2.4.2.2 evolution the enzyme belongs to the NP family II Geobacillus stearothermophilus
2.4.2.2 evolution the enzyme belongs to the NP family II Parageobacillus thermoglucosidasius