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Literature summary extracted from

  • Sternberg, A.R.; Roepe, P.D.
    Heterologous expression, purification, and functional analysis of the Plasmodium falciparum phosphatidylinositol 4-kinase IIIbeta (2020), Biochemistry, 59, 2494-2506 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.67 expressed in Pichia pastoris Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.67 H1484Y the mutant shows reduced activity compared to the wild type enzyme Plasmodium falciparum
2.7.1.67 S1320L the mutant shows reduced activity compared to the wild type enzyme Plasmodium falciparum
2.7.1.67 Y1356F the mutant shows reduced activity compared to the wild type enzyme Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.67 BQR695
-
 Plasmodium falciparum
2.7.1.67 KDU691 complete inhibition at 0.01 mM Plasmodium falciparum
2.7.1.67 MMV390048 complete inhibition at 0.01 mM Plasmodium falciparum
2.7.1.67 NVP-BGT226 complete inhibition at 0.01 mM Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.67 0.0791
-
 ATP at pH 7.4 and 37°C Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.67 Mg2+ 10 mM used in assay conditions Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol Plasmodium falciparum
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Plasmodium falciparum Q8I406
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67 HIS-Select nickel affinity gel chromatography Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Plasmodium falciparum ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.67 ? x * 40000, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.67 phosphatidylinositol 4-kinase IIIbeta
-
 Plasmodium falciparum
2.7.1.67 PI4KIIIbeta
-
 Plasmodium falciparum