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Literature summary extracted from

  • Mothersole, R.; Wolthers, K.
    Structural and kinetic insight into the biosynthesis of H2S and L-lanthionine from L-cysteine by a pyridoxal L-phosphate-dependent enzyme from Fusobacterium nucleatum (2019), Biochemistry, 58, 3592-3603 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 expressed in Escherichia coli BL21(DE3) cells Fusobacterium nucleatum subsp. nucleatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.16
-
 H2S at pH 7.4 and 25°C Fusobacterium nucleatum subsp. nucleatum
2.5.1.47 0.41
-
 O-acetyl-L-serine at pH 7.4 and 25°C Fusobacterium nucleatum subsp. nucleatum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Fusobacterium nucleatum subsp. nucleatum
-
-
-
2.5.1.47 Fusobacterium nucleatum subsp. nucleatum ATCC 25586
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 L-cysteine + L-cysteine preferred reaction Fusobacterium nucleatum subsp. nucleatum L-lanthionine + H2S
-
 ?
2.5.1.47 L-cysteine + L-cysteine preferred reaction Fusobacterium nucleatum subsp. nucleatum ATCC 25586 L-lanthionine + H2S
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Fusobacterium nucleatum subsp. nucleatum L-cysteine + acetate
-
 ir
2.5.1.47 O-acetyl-L-serine
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 L-cysteine + acetate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 Fn1220
-
 Fusobacterium nucleatum subsp. nucleatum
2.5.1.47 lanthionine synthase
-
 Fusobacterium nucleatum subsp. nucleatum
2.5.1.47 OASS
-
 Fusobacterium nucleatum subsp. nucleatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.47 6.3
-
 O-acetyl-L-serine at pH 7.4 and 25°C Fusobacterium nucleatum subsp. nucleatum
2.5.1.47 6.3
-
 H2S at pH 7.4 and 25°C Fusobacterium nucleatum subsp. nucleatum

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate dependent on Fusobacterium nucleatum subsp. nucleatum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.47 15
-
 O-acetyl-L-serine at pH 7.4 and 25°C Fusobacterium nucleatum subsp. nucleatum
2.5.1.47 40
-
 H2S at pH 7.4 and 25°C Fusobacterium nucleatum subsp. nucleatum