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Literature summary extracted from

  • Simithy, J.; Fuanta, N.R.; Alturki, M.; Hobrath, J.V.; Wahba, A.E.; Pina, I.; Rath, J.; Hamann, M.T.; DeRuiter, J.; Goodwin, D.C.; Calderon, A.I.
    Slow-binding inhibition of Mycobacterium tuberculosis shikimate kinase by manzamine alkaloids (2018), Biochemistry, 57, 4923-4933 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.71 6-cyclohexamidomanzamine A noncompetitive inhibition Mycobacterium tuberculosis
2.7.1.71 6-deoxymanzamine X noncompetitive inhibition Mycobacterium tuberculosis
2.7.1.71 8-hydroxymanzamine A noncompetitive inhibition Mycobacterium tuberculosis
2.7.1.71 manzamine A noncompetitive inhibition Mycobacterium tuberculosis
2.7.1.71 manzamine E noncompetitive inhibition Mycobacterium tuberculosis
2.7.1.71 manzamine F noncompetitive inhibition Mycobacterium tuberculosis
2.7.1.71 Rifampin
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Mg2+ 5 mM used in assay conditions Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.71 ATP + shikimate Mycobacterium tuberculosis
-
 ADP + 3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Mycobacterium tuberculosis ADP + 3-phosphoshikimate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.71 0.0024
-
 6-cyclohexamidomanzamine A estimated Ki-value determined with varied shikimate concentrations and a fixed ATP concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.0032
-
 6-cyclohexamidomanzamine A estimated Ki-value determined with varied ATP concentrations and a fixed shikimate concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.011
-
 8-hydroxymanzamine A estimated Ki-value determined with varied ATP concentrations and a fixed shikimate concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.012
-
 6-deoxymanzamine X estimated Ki-value determined with varied shikimate concentrations and a fixed ATP concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.015
-
 8-hydroxymanzamine A estimated Ki-value determined with varied shikimate concentrations and a fixed ATP concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.021
-
 6-deoxymanzamine X estimated Ki-value determined with varied ATP concentrations and a fixed shikimate concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.022
-
 manzamine F estimated Ki-value determined with varied shikimate concentrations and a fixed ATP concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.04
-
 manzamine F estimated Ki-value determined with varied ATP concentrations and a fixed shikimate concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.058
-
 manzamine E estimated Ki-value determined with varied shikimate concentrations and a fixed ATP concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.063
-
 manzamine E estimated Ki-value determined with varied ATP concentrations and a fixed shikimate concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.12
-
 manzamine A estimated Ki-value determined with varied ATP concentrations and a fixed shikimate concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.151
-
 manzamine A estimated Ki-value determined with varied shikimate concentrations and a fixed ATP concentration, at pH 7.6 and 25°C Mycobacterium tuberculosis